##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063001_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	160897
Sequences flagged as poor quality	0
Sequence length	38
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.998303262335533	32.0	32.0	32.0	21.0	32.0
2	29.905169145478162	32.0	32.0	32.0	21.0	32.0
3	30.223130325612036	32.0	32.0	32.0	21.0	32.0
4	30.00773786956873	32.0	32.0	32.0	21.0	32.0
5	30.33788697116789	32.0	32.0	32.0	21.0	32.0
6	33.754408099591664	36.0	36.0	36.0	32.0	36.0
7	33.23043934939744	36.0	36.0	36.0	21.0	36.0
8	33.07187828237941	36.0	36.0	36.0	21.0	36.0
9	32.90901011205927	36.0	36.0	36.0	14.0	36.0
10	33.06190917170612	36.0	36.0	36.0	21.0	36.0
11	33.575162992473444	36.0	36.0	36.0	21.0	36.0
12	33.40200873850973	36.0	36.0	36.0	21.0	36.0
13	33.427273348788354	36.0	36.0	36.0	21.0	36.0
14	33.37695544354463	36.0	36.0	36.0	21.0	36.0
15	33.50703866448722	36.0	36.0	36.0	21.0	36.0
16	33.44828057701511	36.0	36.0	36.0	21.0	36.0
17	33.36061579768423	36.0	36.0	36.0	21.0	36.0
18	33.467578637264836	36.0	36.0	36.0	21.0	36.0
19	33.34961497106845	36.0	36.0	36.0	21.0	36.0
20	32.95246026961348	36.0	36.0	36.0	14.0	36.0
21	32.81769703599197	36.0	36.0	36.0	14.0	36.0
22	32.906511619234664	36.0	36.0	36.0	14.0	36.0
23	33.06456304343773	36.0	36.0	36.0	21.0	36.0
24	33.100517722518134	36.0	36.0	36.0	21.0	36.0
25	33.10736060958253	36.0	36.0	36.0	21.0	36.0
26	33.108497983181785	36.0	36.0	36.0	21.0	36.0
27	33.16008999546293	36.0	36.0	36.0	21.0	36.0
28	33.15912043108324	36.0	36.0	36.0	21.0	36.0
29	33.044991516311676	36.0	36.0	36.0	21.0	36.0
30	32.94610216473893	36.0	36.0	36.0	21.0	36.0
31	32.97295163986898	36.0	36.0	36.0	21.0	36.0
32	32.84598221222273	36.0	36.0	36.0	14.0	36.0
33	32.54611956717652	36.0	36.0	36.0	14.0	36.0
34	32.3643448914523	36.0	36.0	36.0	14.0	36.0
35	32.26761841426503	36.0	36.0	36.0	14.0	36.0
36	32.17748621789095	36.0	32.0	36.0	14.0	36.0
37	32.14540979632933	36.0	32.0	36.0	14.0	36.0
38	31.08972199605959	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	2.0
14	153.0
15	291.0
16	314.0
17	386.0
18	537.0
19	736.0
20	942.0
21	1133.0
22	1340.0
23	1599.0
24	1973.0
25	2351.0
26	2944.0
27	3658.0
28	4816.0
29	6195.0
30	7747.0
31	9809.0
32	12334.0
33	16593.0
34	30525.0
35	54517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.887844884447375	16.39328960959765	13.412069244458824	16.30679626149615
2	14.137450719464722	22.240600942703995	40.97901923961844	22.642929098212843
3	19.886247086247085	35.12229992229992	26.237762237762237	18.753690753690755
4	10.626806302246807	15.771155101152926	33.11849342739053	40.483545169209734
5	11.18106614790829	42.8087534261049	27.439914976662088	18.570265449324726
6	27.04666368337311	35.20286396181384	20.868138424821005	16.882333929992043
7	26.74878959831445	35.15603149841203	19.717583298632043	18.377595604641478
8	34.523328589097375	25.165167778143783	18.597612136957185	21.71389149580166
9	33.19079908264293	12.068590464707235	19.786571533341206	34.95403891930863
10	19.557232266605343	27.65185180581366	26.633809207132515	26.157106720448486
11	33.525796006140574	25.717073655817074	20.386955629999317	20.370174708043034
12	28.403172274913917	27.65236739716832	27.081805412259	16.862654915658755
13	33.40170812665498	22.369124429692064	26.796081502753637	17.433085940899314
14	27.60481088976598	19.243559063927652	30.28374304627529	22.86788700003108
15	25.05346130893177	24.705962800875273	32.88056992241894	17.36000596777402
16	20.174042764793633	28.273868722028837	28.871829935355542	22.68025857782198
17	18.388761111456457	26.051470131161807	29.449244731770996	26.110524025610744
18	19.693457060991605	24.748432168362434	35.342561112803075	20.21554965784289
19	21.57236887085045	25.315897098035318	35.716105935074054	17.395628096040173
20	25.587827632715722	19.728508474786967	36.062751337878915	18.620912554618403
21	27.511793698839586	20.67113760247621	31.652485222914894	20.16458347576931
22	24.147533035814178	23.016297253956218	32.72441355991199	20.111756150317618
23	21.078541060639964	25.35429253375103	33.89833668978891	19.668829715820095
24	19.91931824538939	27.52627751291343	33.95968398610136	18.59472025559582
25	22.521352114076855	25.595186294864302	32.758556385742885	19.12490520531596
26	19.908872215971606	26.519677012301628	34.12754160113879	19.44390917058798
27	20.149796438449822	28.438325512011687	34.185287627808684	17.226590421729806
28	18.223404519802102	27.95229097779877	34.52688760162096	19.29741690077816
29	17.28420385253697	29.27008161311777	35.22168559369969	18.22402894064557
30	18.929232911109178	29.070692505624823	33.838427784752696	18.161646798513306
31	17.674699019833305	31.008571020131644	31.707802176628896	19.60892778340616
32	17.26718420774571	31.371318113730585	32.17808551237189	19.183412166151818
33	17.976194175964196	30.713242378096155	31.196817602635424	20.113745843304223
34	18.184474045711376	30.91826776645802	31.505665748792573	19.391592439038035
35	19.01189136772485	31.465193040472922	31.02136467897037	18.50155091283186
36	18.495266628957165	34.17723879437341	29.63531598281939	17.692178593850034
37	18.421690566319203	35.02402396802606	29.718238946799186	16.836046518855554
38	17.48585990428243	35.92268009198831	29.075766051339425	17.51569395238983
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	232.5
2	291.0
3	291.0
4	773.0
5	1255.0
6	1255.0
7	1723.0
8	2191.0
9	2224.5
10	2258.0
11	2258.0
12	2740.5
13	3223.0
14	3630.0
15	4037.0
16	4037.0
17	5659.5
18	7282.0
19	7282.0
20	7858.5
21	8435.0
22	6309.0
23	4183.0
24	4183.0
25	3300.0
26	2417.0
27	2417.0
28	3408.0
29	4399.0
30	5619.0
31	6839.0
32	6839.0
33	5405.5
34	3972.0
35	3972.0
36	3919.5
37	3867.0
38	4754.0
39	5641.0
40	5641.0
41	6886.0
42	8131.0
43	9730.0
44	11329.0
45	11329.0
46	13906.0
47	16483.0
48	16483.0
49	14829.5
50	13176.0
51	11090.0
52	9004.0
53	9004.0
54	7997.0
55	6990.0
56	6990.0
57	7428.5
58	7867.0
59	7222.5
60	6578.0
61	6578.0
62	6033.5
63	5489.0
64	4993.0
65	4497.0
66	4497.0
67	4079.0
68	3661.0
69	3661.0
70	3211.0
71	2761.0
72	2372.5
73	1984.0
74	1984.0
75	1572.5
76	1161.0
77	1161.0
78	916.5
79	672.0
80	480.5
81	289.0
82	289.0
83	213.0
84	137.0
85	137.0
86	89.5
87	42.0
88	38.5
89	35.0
90	35.0
91	26.5
92	18.0
93	14.0
94	10.0
95	10.0
96	11.0
97	12.0
98	12.0
99	59.5
100	107.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11870948494999906
2	0.0515857971248687
3	0.01367334381623026
4	0.0012430312560209327
5	0.0
6	6.215156280104663E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.001864546884031399
13	0.01180879693219886
14	0.007458187536125596
15	0.02051001572434539
16	0.010565765676177927
17	0.016780921956282592
18	0.003729093768062798
19	0.003729093768062798
20	0.004972125024083731
21	0.003729093768062798
22	0.00683667190811513
23	0.008079703164136063
24	0.011187281304188394
25	0.014294859444240724
26	0.014916375072251192
27	0.007458187536125596
28	0.0031075781400523314
29	0.009944250048167461
30	0.001864546884031399
31	0.003729093768062798
32	0.004972125024083731
33	0.007458187536125596
34	0.011187281304188394
35	0.014916375072251192
36	0.011187281304188394
37	0.009944250048167461
38	0.004350609396073264
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	160897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.023157672299675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.04383707516195	31.115558400715987
2	13.514952524625079	11.358819617519282
3	5.160173928476351	6.505404078385552
4	2.4906084538705002	4.186529270278501
5	1.363622918330523	2.8651870451282497
6	0.8149199869849441	2.0547306662026017
7	0.519123258496761	1.5270638980217157
8	0.3667879433253468	1.2330870059727652
9	0.27065400656668737	1.023636239333238
>10	1.2541781287898957	9.527213061772438
>50	0.08134410033425031	2.4382058086850593
>100	0.09613393675865944	8.665170885721922
>500	0.008873901854645488	2.689919638029298
>1k	0.01331085278196823	10.918165037259863
>5k	0.0014789836424409146	3.8913093469735296
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6261	3.8913093469735296	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4308	2.677489325469089	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2644	1.643287320459673	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2284	1.4195416943759052	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2004	1.2455173185329744	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1459	0.9067913012672704	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1359	0.8446397384662238	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1273	0.7911893944573237	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	1168	0.7259302535162246	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1068	0.663778690715178	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	948	0.589196815353922	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	766	0.4760809710560172	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	722	0.4487342834235567	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	660	0.41020031448690775	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	655	0.4070927363468554	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	577	0.3586145173620391	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	497	0.3088932671212018	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	486	0.3020565952130866	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	475	0.2952199233049715	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	417	0.25917201688036445	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	416	0.258550501252354	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	407	0.2529568606002598	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	384	0.23866200115601907	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	378	0.2349329073879563	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	346	0.21504440729162136	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.2044786416154434	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	305	0.18956226654319222	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	298	0.18521165714711896	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	286	0.17775346961099336	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	266	0.16532315705078404	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	265	0.16470164142277358	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	256	0.15910800077067938	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	254	0.15786496951465845	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	253	0.15724345388664798	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	252	0.15662193825863752	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	252	0.15662193825863752	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	250	0.15537890700261658	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	236	0.14667768821047006	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	236	0.14667768821047006	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	235	0.1460561725824596	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	231	0.14357011007041773	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	213	0.13238282876622934	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	195	0.12119554746204093	No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA	192	0.11933100057800954	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	186	0.11560190680994674	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	184	0.11435887555392579	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	183	0.11373735992591533	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	180	0.11187281304188394	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	179	0.11125129741387348	No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	178	0.110629781785863	No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA	173	0.10752220364581068	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	172	0.1069006880178002	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	172	0.1069006880178002	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	169	0.10503614113376882	No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA	166	0.1031715942497374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	6.215156280104663E-4	0.0
17	0.0	0.0	0.0	0.0012430312560209327	0.0
18	0.0	0.0	0.0	0.0012430312560209327	0.0
19	0.0	0.0	0.0	0.001864546884031399	0.0
20	0.0	0.0	0.0	0.0031075781400523314	0.0
21	0.0	0.0	0.0	0.006215156280104663	0.0
22	0.0	0.0	0.0	0.008701218792146529	0.0
23	0.0	0.0	0.0	0.016780921956282592	0.0
24	0.0	0.0	0.0	0.03294032828455472	0.0
25	0.0	0.0	0.0	0.041641547076701244	0.0
26	0.0	0.0	0.0	0.0515857971248687	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCCCA	20	0.0037447158	31.998756	13
GGTCTAC	30	8.3567534E-4	26.682219	1
CTGCTTA	30	8.3871826E-4	26.66563	25
TGGGTAG	40	0.0044680294	19.999224	6
TAGGACC	40	0.0044680294	19.999224	4
ATGGGTA	60	1.1308086E-4	18.665941	5
GGAGAGT	45	0.008820129	17.777086	8
GCCGCCC	45	0.008820129	17.777086	12
AAGTACT	65	2.0819539E-4	17.235458	2
TACATAG	60	0.0024252606	16.004354	2
AGTACTC	70	3.6641644E-4	16.004353	3
TGGGAGA	65	0.004141999	14.768658	6
CCTTGGC	80	0.001009196	14.00381	2
CATGGGT	575	0.0	12.799502	4
TGGGTGG	405	0.0	12.641485	6
CTTCCAT	385	0.0	12.467049	28
GCTTCCA	385	0.0	12.467049	27
TTCCATG	400	0.0	12.399519	29
ATGGAAG	530	0.0	12.376879	5
ATGGGTG	470	0.0	12.254843	5
>>END_MODULE