FastQCFastQC Report
Thu 2 Feb 2017
SRR4063001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160897
Sequences flagged as poor quality0
Sequence length38
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT74464.627805366165933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT56223.4941608606748416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT33412.076483713182968No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25461.5823787889146474No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA21751.3517964909227642No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13450.8359385196740772No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13230.822265175857847No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11830.7352529879363817No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA11800.7333884410523503No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA11030.6855317376955444No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.589196815353922No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA9130.5674437683735558No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA8560.5320173775769592No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8370.5202085806447604No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.4748379397999962No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA6310.3921763612746042No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5620.34929178294188207No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.3356184391256518No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5380.3343754078696309No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA4530.28154657948874123No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4410.27408839195261564No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA4040.2510923137162284No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4010.249227766832197No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA3930.24425564180811324No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA3850.23928351678402954No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC3810.23679745427198765No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA3650.22685320422382022No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3620.22498865733978882No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA3540.22001653231570506No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG3360.2088292510115167No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3330.2069647041274853No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA3110.19329136031125502No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3060.1901837821712027No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2930.18210407900706663No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA2900.18023953212303523No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2770.17215982895889917No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2720.16905225081884684No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA2690.16718770393481544No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC2670.1659446726787945No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT2520.15662193825863752No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA2340.14543465695444913No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2300.14294859444240723No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT2300.14294859444240723No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT2300.14294859444240723No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2250.13984101630235493No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2220.13797646941832353No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT2180.13549040690628164No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2150.13362586002225027No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2110.13113979751020838No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT2000.12430312560209326No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA2000.12430312560209326No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1990.1236816099740828No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG1960.12181706309005141No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1920.11933100057800954No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC1830.11373735992591533No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA1810.11249432866989441No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1770.11000826615785253No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT1760.10938675052984208No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT1690.10503614113376882No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1660.1031715942497374No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1610.10006401610968509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG200.003744003432.01
CTCTAGA200.003744003432.01
ATCATCC308.385281E-426.6666663
ATACATG502.7783404E-522.41
GGAGGCT400.00446702520.025
GGTTTAA450.00881815417.77777932
AGGGTGT450.00881815417.7777796
CTGCGCG550.001357743617.4545469
CAGCACC600.00242967316.04
AAAAAGT650.0041409314.7692311
CCAGCAC800.001011275914.03
AGTACTC700.00677035313.7142875
GGGGGTA900.002455648312.4444457
TTTTTGT1108.2816044E-411.63636430
TGGAAGC4850.011.54639156
ATGGAAG4950.011.3131315
CATGGAA5100.011.2941174
GGGGTGG8400.010.6666677
TTTGGTT1353.7991174E-410.66666731
TGGGGTG8400.010.6666676
Produced by FastQC (version 0.11.5)