##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063001_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	160897
Sequences flagged as poor quality	0
Sequence length	38
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.892229190102984	32.0	32.0	32.0	21.0	32.0
2	31.149834987600762	32.0	32.0	32.0	32.0	32.0
3	31.28743854764228	32.0	32.0	32.0	32.0	32.0
4	31.472327016662835	32.0	32.0	32.0	32.0	32.0
5	31.19835049752326	32.0	32.0	32.0	32.0	32.0
6	34.65432543801314	36.0	36.0	36.0	32.0	36.0
7	34.76011982821308	36.0	36.0	36.0	32.0	36.0
8	34.77783923876766	36.0	36.0	36.0	32.0	36.0
9	34.800220016532315	36.0	36.0	36.0	32.0	36.0
10	34.66284020211688	36.0	36.0	36.0	32.0	36.0
11	34.83556561029727	36.0	36.0	36.0	32.0	36.0
12	34.7884795863192	36.0	36.0	36.0	32.0	36.0
13	34.774029347967954	36.0	36.0	36.0	32.0	36.0
14	34.68018670329465	36.0	36.0	36.0	32.0	36.0
15	34.6829213720579	36.0	36.0	36.0	32.0	36.0
16	34.642212098423215	36.0	36.0	36.0	32.0	36.0
17	34.610222689049515	36.0	36.0	36.0	32.0	36.0
18	34.54388832607196	36.0	36.0	36.0	32.0	36.0
19	34.468554416800814	36.0	36.0	36.0	32.0	36.0
20	34.365395252863635	36.0	36.0	36.0	32.0	36.0
21	34.290036482967366	36.0	36.0	36.0	32.0	36.0
22	34.34076458852558	36.0	36.0	36.0	32.0	36.0
23	34.349316643567	36.0	36.0	36.0	32.0	36.0
24	34.32883770362406	36.0	36.0	36.0	32.0	36.0
25	34.29627649987259	36.0	36.0	36.0	32.0	36.0
26	34.25301279700678	36.0	36.0	36.0	32.0	36.0
27	34.24001690522508	36.0	36.0	36.0	32.0	36.0
28	34.2009297873795	36.0	36.0	36.0	32.0	36.0
29	34.1010584411145	36.0	36.0	36.0	32.0	36.0
30	34.036619700802376	36.0	36.0	36.0	32.0	36.0
31	33.91858766788691	36.0	36.0	36.0	32.0	36.0
32	33.80836187125925	36.0	36.0	36.0	32.0	36.0
33	33.56089299365433	36.0	36.0	36.0	27.0	36.0
34	33.323212987190566	36.0	36.0	36.0	21.0	36.0
35	33.1735644542782	36.0	36.0	36.0	14.0	36.0
36	33.08529680478816	36.0	36.0	36.0	14.0	36.0
37	32.98573000118088	36.0	36.0	36.0	14.0	36.0
38	32.617817610023806	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	19.0
23	44.0
24	173.0
25	419.0
26	936.0
27	1798.0
28	3175.0
29	4851.0
30	7058.0
31	9901.0
32	12980.0
33	19130.0
34	36935.0
35	63475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.77976889750809	17.89210658809928	14.348493607076037	16.979630907316587
2	14.118349005885753	23.05698676793228	38.890097391498905	23.934566834683057
3	19.85555976805037	33.761350429156536	27.262161507051097	19.120928295741997
4	10.58316811376222	16.307327047738614	32.73274206479922	40.376762773699944
5	10.978452053176877	42.03310192234784	28.206865261627	18.781580762848282
6	27.701573056054496	35.47113992181333	19.198617749243304	17.628669272888867
7	25.277819212410503	34.813171241050114	20.392676014319807	19.51633353221957
8	33.72965313212801	25.808436453134615	18.93012299794278	21.531787416794597
9	31.530467642890887	11.485344206051264	20.459065708674142	36.52512244238371
10	19.351871418449416	27.605752855917558	27.13028453515979	25.91209119047323
11	33.95777265353562	24.61293670870341	20.26092199066449	21.16836864709648
12	27.06982814879269	27.98968271232792	28.035675440504676	16.904813698374717
13	33.77129467920471	21.58337321391946	27.646258165161562	16.999073941714265
14	26.95140369304586	18.864242341373675	31.39772649583274	22.786627469747728
15	25.5225392642498	24.0855951322896	32.944057378322775	17.44780822513782
16	20.296835863937797	26.592789175683823	29.993101176529084	23.117273783849296
17	18.4739305269831	24.798473557617605	31.384053152016506	25.343542763382786
18	20.140213925679163	21.935772575001398	37.565026072580594	20.358987426738846
19	21.567213807591195	23.359043363145364	38.18840624747509	16.885336581788348
20	24.556082462693524	18.160686650465827	39.25865615890912	18.024574727931533
21	26.32678048689534	19.383829406390422	34.661926574143706	19.627463532570527
22	23.16202290906605	21.265778728006115	35.97270303361778	19.599495329310056
23	20.322939520314236	22.97743276754694	37.03735930439971	19.662268407739113
24	19.506889500736495	24.901645151867342	36.962777429038454	18.628687918357706
25	21.86802737154826	23.009130064575473	35.94224876784527	19.180593796031
26	19.389423047042516	23.808399162196935	37.48733661907929	19.314841171681262
27	20.315481332778113	25.39512856050765	37.11380572664499	17.175584380069235
28	18.536703605412157	24.438616008999546	37.59548033835311	19.429200047235188
29	17.314803880743582	25.879910750355815	38.13495590346619	18.670329465434406
30	18.92887996668676	25.6710814993443	37.24743158666725	18.152606947301688
31	17.956829524478394	27.052710740411566	35.08517871681883	19.905281018291205
32	17.43227033443756	27.47161227369062	35.686184329104954	19.409933062766864
33	18.253292479039384	27.01106919333487	34.7744209028136	19.961217424812148
34	18.32476677626059	27.250352710118896	35.095744482495014	19.3291360311255
35	19.60135987619409	27.769939775135644	34.234945337700516	18.393755010969752
36	19.142681342722362	29.935300223124113	33.05157958196859	17.87043885218494
37	19.426092469095135	30.732083258233526	33.050336550712565	16.79148772195877
38	18.340926182588863	31.16030752593274	32.741443283591366	17.75732300788703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	86.0
2	110.0
3	110.0
4	519.0
5	928.0
6	928.0
7	1439.0
8	1950.0
9	2266.0
10	2582.0
11	2582.0
12	3128.0
13	3674.0
14	4116.5
15	4559.0
16	4559.0
17	6608.5
18	8658.0
19	8658.0
20	8915.5
21	9173.0
22	6993.5
23	4814.0
24	4814.0
25	3295.5
26	1777.0
27	1777.0
28	2672.5
29	3568.0
30	4580.0
31	5592.0
32	5592.0
33	4534.0
34	3476.0
35	3476.0
36	3398.5
37	3321.0
38	4085.0
39	4849.0
40	4849.0
41	6278.5
42	7708.0
43	9268.0
44	10828.0
45	10828.0
46	14033.0
47	17238.0
48	17238.0
49	15808.0
50	14378.0
51	11903.5
52	9429.0
53	9429.0
54	8308.0
55	7187.0
56	7187.0
57	7462.5
58	7738.0
59	7124.5
60	6511.0
61	6511.0
62	6024.5
63	5538.0
64	5063.0
65	4588.0
66	4588.0
67	4180.5
68	3773.0
69	3773.0
70	3239.5
71	2706.0
72	2312.5
73	1919.0
74	1919.0
75	1514.5
76	1110.0
77	1110.0
78	868.0
79	626.0
80	465.0
81	304.0
82	304.0
83	222.5
84	141.0
85	141.0
86	90.5
87	40.0
88	31.5
89	23.0
90	23.0
91	13.0
92	3.0
93	4.5
94	6.0
95	6.0
96	6.0
97	6.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009944250048167461
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	6.215156280104663E-4
8	0.0
9	0.0031075781400523314
10	0.001864546884031399
11	0.003729093768062798
12	0.0012430312560209327
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	160897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.5414084787162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.13388407860538	26.704661988725707
2	14.901577736205155	11.188524335444415
3	5.806003013095376	6.538965922298116
4	2.736619042100558	4.109461332405203
5	1.5231031571279574	2.8589718888481452
6	0.9552505670248167	2.1516871041722343
7	0.5844080592023575	1.5357651168138622
8	0.3774646954621459	1.1336445054910906
9	0.27647633395692267	0.9341379888997309
>10	1.443636905451716	9.627277077882123
>50	0.11423273678459679	2.9006134359248463
>100	0.11588828369451848	9.27612074805621
>500	0.014899922189295233	4.095166472960963
>1k	0.01324437527937354	8.82303585523658
>5k	0.003311093819843385	8.121966226840774
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7446	4.627805366165933	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5622	3.4941608606748416	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3341	2.076483713182968	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2546	1.5823787889146474	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2175	1.3517964909227642	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1345	0.8359385196740772	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1323	0.822265175857847	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1183	0.7352529879363817	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1180	0.7333884410523503	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1103	0.6855317376955444	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	948	0.589196815353922	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	913	0.5674437683735558	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	856	0.5320173775769592	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	837	0.5202085806447604	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	764	0.4748379397999962	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	631	0.3921763612746042	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	562	0.34929178294188207	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.3356184391256518	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	538	0.3343754078696309	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	453	0.28154657948874123	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	441	0.27408839195261564	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	404	0.2510923137162284	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	401	0.249227766832197	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	393	0.24425564180811324	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	385	0.23928351678402954	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	381	0.23679745427198765	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	365	0.22685320422382022	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	362	0.22498865733978882	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	354	0.22001653231570506	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	336	0.2088292510115167	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	333	0.2069647041274853	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	311	0.19329136031125502	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	306	0.1901837821712027	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	293	0.18210407900706663	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	290	0.18023953212303523	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	277	0.17215982895889917	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	272	0.16905225081884684	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	269	0.16718770393481544	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	267	0.1659446726787945	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	252	0.15662193825863752	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	234	0.14543465695444913	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	230	0.14294859444240723	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	230	0.14294859444240723	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	230	0.14294859444240723	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	225	0.13984101630235493	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	222	0.13797646941832353	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	218	0.13549040690628164	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	215	0.13362586002225027	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	211	0.13113979751020838	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	200	0.12430312560209326	No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA	200	0.12430312560209326	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	199	0.1236816099740828	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	196	0.12181706309005141	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	192	0.11933100057800954	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	183	0.11373735992591533	No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA	181	0.11249432866989441	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	177	0.11000826615785253	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT	176	0.10938675052984208	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT	169	0.10503614113376882	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	166	0.1031715942497374	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	161	0.10006401610968509	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	6.215156280104663E-4	0.0
17	0.0	0.0	0.0	0.0012430312560209327	0.0
18	0.0	0.0	0.0	0.0012430312560209327	0.0
19	0.0	0.0	0.0	0.001864546884031399	0.0
20	0.0	0.0	0.0	0.0043506093960732645	0.0
21	0.0	0.0	0.0	0.007458187536125596	0.0
22	0.0	0.0	0.0	0.009944250048167461	0.0
23	0.0	0.0	0.0	0.018023953212303524	0.0
24	0.0	0.0	0.0	0.03418335954057565	0.0
25	0.0	0.0	0.0	0.041641547076701244	0.0
26	0.0	0.0	0.0	0.050964281496858235	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACG	20	0.0037440034	32.0	1
CTCTAGA	20	0.0037440034	32.0	1
ATCATCC	30	8.385281E-4	26.666666	3
ATACATG	50	2.7783404E-5	22.4	1
GGAGGCT	40	0.004467025	20.0	25
GGTTTAA	45	0.008818154	17.777779	32
AGGGTGT	45	0.008818154	17.777779	6
CTGCGCG	55	0.0013577436	17.454546	9
CAGCACC	60	0.002429673	16.0	4
AAAAAGT	65	0.00414093	14.769231	1
CCAGCAC	80	0.0010112759	14.0	3
AGTACTC	70	0.006770353	13.714287	5
GGGGGTA	90	0.0024556483	12.444445	7
TTTTTGT	110	8.2816044E-4	11.636364	30
TGGAAGC	485	0.0	11.5463915	6
ATGGAAG	495	0.0	11.313131	5
CATGGAA	510	0.0	11.294117	4
GGGGTGG	840	0.0	10.666667	7
TTTGGTT	135	3.7991174E-4	10.666667	31
TGGGGTG	840	0.0	10.666667	6
>>END_MODULE