FastQCFastQC Report
Thu 2 Feb 2017
SRR4063000_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063000_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828438
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31800.38385491732634186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21220.25614469640455895No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16080.1941002223461502No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13590.16404365806493665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12110.1461787122271069No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9620.11612214794589336No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9100.10984527508395318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATAC453.5424266E-421.327263
AGACCGA453.5438716E-421.3259726
CCGATAA601.1370196E-418.668119
TCTAGCG1550.018.57871628
CTAGCGG1650.018.4234429
GTCCTAT1401.8189894E-1218.2959671
GCCCTAA450.00881971417.7877461
AACCGTG550.001366263417.4506267
CGATAAC652.0982699E-417.23210110
CGGTCCG652.1011765E-417.22898126
GGATAGA854.9540267E-616.9506761
TAGCGGC1700.016.94044130
GATATAC957.641065E-716.8515471
GTGCTAC703.6791008E-416.0089721
GATAACG703.6926623E-416.00123811
CGTCGTA1001.3122244E-616.00123810
ATCGTTT1304.796675E-915.99930529
ACTGATC703.697759E-415.9983418
ACCGTCG1102.0195512E-715.998348
AACTATG600.002444793515.9964087
Produced by FastQC (version 0.11.5)