##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063000_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	828438
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249387401350493	32.0	32.0	32.0	32.0	32.0
2	30.922655648340612	32.0	32.0	32.0	32.0	32.0
3	30.981008838319827	32.0	32.0	32.0	32.0	32.0
4	31.025021787991378	32.0	32.0	32.0	32.0	32.0
5	30.97540793638148	32.0	32.0	32.0	32.0	32.0
6	34.61809453453366	36.0	36.0	36.0	32.0	36.0
7	34.55193388038695	36.0	36.0	36.0	32.0	36.0
8	34.51796996274918	36.0	36.0	36.0	32.0	36.0
9	34.639138957894254	36.0	36.0	36.0	32.0	36.0
10	34.402908847735134	36.0	36.0	36.0	32.0	36.0
11	34.65187738853119	36.0	36.0	36.0	32.0	36.0
12	34.48974938378008	36.0	36.0	36.0	32.0	36.0
13	34.55915469836005	36.0	36.0	36.0	32.0	36.0
14	34.46364845649282	36.0	36.0	36.0	32.0	36.0
15	34.43352188093738	36.0	36.0	36.0	32.0	36.0
16	34.4463701568494	36.0	36.0	36.0	32.0	36.0
17	34.38199841146833	36.0	36.0	36.0	32.0	36.0
18	34.3999200905801	36.0	36.0	36.0	32.0	36.0
19	34.38342277877162	36.0	36.0	36.0	32.0	36.0
20	34.34255309389478	36.0	36.0	36.0	32.0	36.0
21	34.327615343574294	36.0	36.0	36.0	32.0	36.0
22	34.31310731762667	36.0	36.0	36.0	32.0	36.0
23	34.26510855368778	36.0	36.0	36.0	32.0	36.0
24	34.24744639912703	36.0	36.0	36.0	32.0	36.0
25	34.217645738124034	36.0	36.0	36.0	32.0	36.0
26	34.17839838346382	36.0	36.0	36.0	32.0	36.0
27	34.17630528778255	36.0	36.0	36.0	32.0	36.0
28	34.156655054451875	36.0	36.0	36.0	32.0	36.0
29	34.121627689700375	36.0	36.0	36.0	32.0	36.0
30	34.077426433842966	36.0	36.0	36.0	32.0	36.0
31	34.101727588546154	36.0	36.0	36.0	32.0	36.0
32	34.05558895173809	36.0	36.0	36.0	32.0	36.0
33	34.00174666058293	36.0	36.0	36.0	32.0	36.0
34	34.00088721183722	36.0	36.0	36.0	32.0	36.0
35	33.95829983655989	36.0	36.0	36.0	32.0	36.0
36	33.902882291734564	36.0	36.0	36.0	32.0	36.0
37	33.898375014183316	36.0	36.0	36.0	32.0	36.0
38	33.285105222116805	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	3.0
9	2.0
10	7.0
11	9.0
12	3.0
13	1.0
14	125.0
15	217.0
16	276.0
17	349.0
18	454.0
19	575.0
20	735.0
21	992.0
22	1438.0
23	2155.0
24	3324.0
25	4965.0
26	7099.0
27	10401.0
28	14794.0
29	20599.0
30	28092.0
31	38082.0
32	52488.0
33	78529.0
34	177197.0
35	385524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.1233541401043	16.831489325703515	11.301400076137071	25.743756458055124
2	16.27708973654495	19.360655044774678	38.20973751109554	26.152517707584828
3	19.287268179261023	23.831932529631768	28.279896756622332	28.600902534484884
4	12.343661162541691	15.116566816632847	35.94118364237955	36.59858837844591
5	14.205683963516796	36.99127029728307	33.80550821065236	14.997537528547769
6	34.406524124461484	35.367977856990244	16.853988192165207	13.371509826383063
7	30.323730108626247	30.431764877715505	20.631623165068678	18.612881848589574
8	28.318403540177663	33.47355790993357	19.095690297223793	19.112348252664972
9	27.69233554835322	14.120721601574052	18.32877654855239	39.858166301520335
10	15.689138409503242	27.096337301869916	32.11279608610473	25.10172820252211
11	37.60915383296838	21.251469668693822	21.851770483017027	19.28760601532077
12	24.791741357311594	23.83533896576241	29.07219431759609	22.300725359329903
13	30.10677136151595	19.608191116390923	25.106843797989164	25.17819372410396
14	23.357823727111178	19.71456578845708	25.454999806845397	31.47261067758634
15	25.082676010253152	27.38166675319626	22.871341760108567	24.664315476442017
16	25.23960542819926	25.937627136650242	24.53192586145078	24.29084157369972
17	23.597350571201588	26.06905611080659	25.8412274495505	24.492365868441325
18	24.477450865593664	24.96405682823691	27.10036685574807	23.458125450421356
19	25.086948176351786	25.28360014341469	26.006830305890986	23.622621374342533
20	25.39630804591041	24.366607274781625	26.234916634073556	24.002168045234402
21	26.15595405517966	24.608002317762875	24.91401944747913	24.322024179578335
22	25.385933991646347	24.737680774523767	25.53067432820686	24.345710905623022
23	23.89483303736665	24.901642886719404	26.027483253034013	25.176040822879937
24	24.338677995854173	25.516990763042024	25.816639563058448	24.32769167804535
25	24.728807113493545	24.918355396994997	25.788587261630962	24.5642502278805
26	24.02245630980049	25.66261205517491	26.263740907307355	24.051190727717245
27	24.803288385624782	25.504303632434784	25.467967212718957	24.224440769221484
28	23.947946594558054	25.25918056930394	26.04094739129385	24.751925444844154
29	23.882469119109935	25.532266787711382	26.224974165322617	24.36028992785606
30	24.134788329450394	25.5804492944315	26.307021885298344	23.977740490819762
31	24.499480316614537	25.442877681862026	25.352823371628546	24.70481862989489
32	23.9278156496	25.742685413319265	25.487971085698696	24.841527851382036
33	23.59481384905114	25.47394852479598	25.988459124052344	24.942778502100534
34	24.280573151136075	25.46512091151747	26.26540292370339	23.988903013643064
35	24.81960234646732	25.526947069270044	25.969656251471374	23.683794332791265
36	23.832548593504768	26.115175660992396	25.66123385246891	24.391041893033925
37	24.712323144526536	25.75546863682457	25.387391955188566	24.14481626346033
38	23.897612701355893	26.115304013597555	25.629056089929307	24.35802719511724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	87.0
2	120.0
3	120.0
4	351.0
5	582.0
6	582.0
7	772.5
8	963.0
9	984.5
10	1006.0
11	1006.0
12	1284.0
13	1562.0
14	2039.0
15	2516.0
16	2516.0
17	3591.5
18	4667.0
19	4667.0
20	5555.5
21	6444.0
22	6626.5
23	6809.0
24	6809.0
25	7979.0
26	9149.0
27	9149.0
28	11821.5
29	14494.0
30	18157.5
31	21821.0
32	21821.0
33	26324.0
34	30827.0
35	30827.0
36	34205.0
37	37583.0
38	42521.0
39	47459.0
40	47459.0
41	50381.5
42	53304.0
43	58969.5
44	64635.0
45	64635.0
46	69176.0
47	73717.0
48	73717.0
49	75056.5
50	76396.0
51	75550.5
52	74705.0
53	74705.0
54	71799.5
55	68894.0
56	68894.0
57	65959.0
58	63024.0
59	56708.5
60	50393.0
61	50393.0
62	46209.0
63	42025.0
64	35135.0
65	28245.0
66	28245.0
67	23404.0
68	18563.0
69	18563.0
70	14951.0
71	11339.0
72	8887.0
73	6435.0
74	6435.0
75	4920.5
76	3406.0
77	3406.0
78	3063.0
79	2720.0
80	2143.0
81	1566.0
82	1566.0
83	1482.0
84	1398.0
85	1398.0
86	950.5
87	503.0
88	425.5
89	348.0
90	348.0
91	253.0
92	158.0
93	138.5
94	119.0
95	119.0
96	110.5
97	102.0
98	102.0
99	244.5
100	387.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1186570389093692
2	0.0486457646800364
3	0.012553745723880362
4	0.00350056371146664
5	2.4141818699769928E-4
6	6.035454674942482E-4
7	1.2070909349884964E-4
8	6.035454674942482E-4
9	3.621272804965489E-4
10	6.035454674942482E-4
11	0.002414181869976993
12	0.0025348909634758427
13	0.015209345780855055
14	0.01038098204090107
15	0.024745364167264174
16	0.011708782069388414
17	0.022210473203788335
18	0.005673327394445933
19	0.008570345638418323
20	0.005069781926951685
21	0.00639758195543903
22	0.00700112742293328
23	0.009536018386409122
24	0.0162957276223447
25	0.018468491305323995
26	0.019675582240312493
27	0.008208218357921777
28	0.00700112742293328
29	0.011346654788891866
30	0.0033798546179677896
31	0.005914745581443633
32	0.007121836516432129
33	0.009415309292910273
34	0.012674454817379211
35	0.015571473061351605
36	0.016657854902841253
37	0.009415309292910273
38	0.006518291048937881
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	828438.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.715312044376205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.29700904970733	47.538273365943276
2	13.633579907725613	16.55534116758997
3	4.1784648668614	7.610903947738584
4	1.5711946123154887	3.815822846767104
5	0.7458252539706023	2.2641506512700627
6	0.40760532283065554	1.484873061996717
7	0.23303546067648334	0.9904174498664353
8	0.14771620158808618	0.7174908218744507
9	0.11664593419660158	0.6373974864108994
>10	0.5361434944190752	6.090985703803025
>50	0.06788882374527921	2.911969890424696
>100	0.06089777524279223	7.01808092428235
>500	0.002994972540445965	1.2150595063329148
>1k	9.983241801486552E-4	1.1492331756994945
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3180	0.38385491732634186	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2122	0.25614469640455895	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1608	0.1941002223461502	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1359	0.16404365806493665	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1211	0.1461787122271069	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	962	0.11612214794589336	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	910	0.10984527508395318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4141818699769928E-4
2	0.0	0.0	0.0	0.0	2.4141818699769928E-4
3	0.0	0.0	0.0	0.0	2.4141818699769928E-4
4	0.0	0.0	0.0	0.0	2.4141818699769928E-4
5	0.0	0.0	0.0	0.0	2.4141818699769928E-4
6	0.0	0.0	0.0	1.2070909349884964E-4	2.4141818699769928E-4
7	0.0	0.0	0.0	1.2070909349884964E-4	2.4141818699769928E-4
8	0.0	0.0	0.0	1.2070909349884964E-4	2.4141818699769928E-4
9	0.0	0.0	0.0	1.2070909349884964E-4	2.4141818699769928E-4
10	0.0	0.0	0.0	1.2070909349884964E-4	2.4141818699769928E-4
11	0.0	0.0	0.0	1.2070909349884964E-4	2.4141818699769928E-4
12	0.0	0.0	0.0	1.2070909349884964E-4	8.449636544919475E-4
13	0.0	0.0	0.0	1.2070909349884964E-4	0.0010863818414896468
14	0.0	0.0	0.0	2.4141818699769928E-4	0.0012070909349884964
15	0.0	0.0	0.0	2.4141818699769928E-4	0.001327800028487346
16	0.0	0.0	0.0	2.4141818699769928E-4	0.001327800028487346
17	0.0	0.0	0.0	2.4141818699769928E-4	0.001327800028487346
18	0.0	0.0	0.0	3.621272804965489E-4	0.0014485091219861956
19	0.0	0.0	0.0	3.621272804965489E-4	0.0014485091219861956
20	0.0	0.0	0.0	3.621272804965489E-4	0.0014485091219861956
21	0.0	0.0	0.0	6.035454674942482E-4	0.001689927308983895
22	0.0	0.0	0.0	6.035454674942482E-4	0.0018106364024827446
23	0.0	0.0	0.0	0.0014485091219861956	0.0019313454959815942
24	0.0	0.0	0.0	0.003017727337471241	0.0019313454959815942
25	0.0	0.0	0.0	0.0036212728049654893	0.0019313454959815942
26	0.0	0.0	0.0	0.004345527365958587	0.0019313454959815942
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAATAC	45	3.5424266E-4	21.32726	3
AGACCGA	45	3.5438716E-4	21.325972	6
CCGATAA	60	1.1370196E-4	18.66811	9
TCTAGCG	155	0.0	18.578716	28
CTAGCGG	165	0.0	18.42344	29
GTCCTAT	140	1.8189894E-12	18.295967	1
GCCCTAA	45	0.008819714	17.787746	1
AACCGTG	55	0.0013662634	17.450626	7
CGATAAC	65	2.0982699E-4	17.232101	10
CGGTCCG	65	2.1011765E-4	17.228981	26
GGATAGA	85	4.9540267E-6	16.950676	1
TAGCGGC	170	0.0	16.940441	30
GATATAC	95	7.641065E-7	16.851547	1
GTGCTAC	70	3.6791008E-4	16.008972	1
GATAACG	70	3.6926623E-4	16.001238	11
CGTCGTA	100	1.3122244E-6	16.001238	10
ATCGTTT	130	4.796675E-9	15.999305	29
ACTGATC	70	3.697759E-4	15.998341	8
ACCGTCG	110	2.0195512E-7	15.99834	8
AACTATG	60	0.0024447935	15.996408	7
>>END_MODULE