FastQCFastQC Report
Thu 2 Feb 2017
SRR4063000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828438
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34670.4184984271605117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25510.30792889751556546No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17070.20605042260253634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16040.19361738597215483No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15400.18589200398822844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.12831376638927716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCGT200.003753470531.99903518
CGACCCA953.786954E-818.52575717
TCTAGCG1401.8189894E-1218.28516228
GATATAC1500.018.1349751
AGCGTAT1251.4733814E-1017.9216238
CTATACT904.3589534E-717.7793874
AATAGCG1453.6379788E-1217.656775
CTAGCGG1453.6379788E-1217.6546429
CGGTCGC550.001364485817.4540210
TAGCGTA1302.582965E-1017.2323287
CGAACCC753.2327676E-517.0661510
CGACGAC957.68845E-716.84159714
ACGACCC1051.1882912E-716.761416
ATAGCGT1354.4201443E-1016.5940956
CGTTTAT1651.8189894E-1216.4843531
GTCCTAT1703.6379788E-1216.001451
GTCTTAC805.6134697E-516.001451
TTAGGCA600.002439658416.0014484
TAGGACG2800.016.0014484
TATAATA1102.0164771E-716.0004832
Produced by FastQC (version 0.11.5)