##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	828438
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.153960827484976	32.0	32.0	32.0	32.0	32.0
2	31.282901074069514	32.0	32.0	32.0	32.0	32.0
3	31.37984737542218	32.0	32.0	32.0	32.0	32.0
4	31.479452898104626	32.0	32.0	32.0	32.0	32.0
5	31.39586667922041	32.0	32.0	32.0	32.0	32.0
6	34.927570922627886	36.0	36.0	36.0	36.0	36.0
7	34.96442340887308	36.0	36.0	36.0	36.0	36.0
8	34.900868864055006	36.0	36.0	36.0	36.0	36.0
9	35.004490378278156	36.0	36.0	36.0	36.0	36.0
10	34.85279043211441	36.0	36.0	36.0	32.0	36.0
11	35.02479123362279	36.0	36.0	36.0	36.0	36.0
12	34.93773221411862	36.0	36.0	36.0	36.0	36.0
13	34.984211250570354	36.0	36.0	36.0	36.0	36.0
14	34.9283096622801	36.0	36.0	36.0	32.0	36.0
15	34.90630318744432	36.0	36.0	36.0	32.0	36.0
16	34.90808847493717	36.0	36.0	36.0	32.0	36.0
17	34.88750636740468	36.0	36.0	36.0	32.0	36.0
18	34.87896740613057	36.0	36.0	36.0	32.0	36.0
19	34.8718890248878	36.0	36.0	36.0	32.0	36.0
20	34.85724821893733	36.0	36.0	36.0	32.0	36.0
21	34.838208773619755	36.0	36.0	36.0	32.0	36.0
22	34.83015023453777	36.0	36.0	36.0	32.0	36.0
23	34.76379765293239	36.0	36.0	36.0	32.0	36.0
24	34.743838404322354	36.0	36.0	36.0	32.0	36.0
25	34.72670254140925	36.0	36.0	36.0	32.0	36.0
26	34.67357243390573	36.0	36.0	36.0	32.0	36.0
27	34.64939440247792	36.0	36.0	36.0	32.0	36.0
28	34.60338371730896	36.0	36.0	36.0	32.0	36.0
29	34.569876080044615	36.0	36.0	36.0	32.0	36.0
30	34.5421552367226	36.0	36.0	36.0	32.0	36.0
31	34.53043679792574	36.0	36.0	36.0	32.0	36.0
32	34.48931603813442	36.0	36.0	36.0	32.0	36.0
33	34.46183057754473	36.0	36.0	36.0	32.0	36.0
34	34.44545880319348	36.0	36.0	36.0	32.0	36.0
35	34.39902322201541	36.0	36.0	36.0	32.0	36.0
36	34.36289378324027	36.0	36.0	36.0	32.0	36.0
37	34.34388692937794	36.0	36.0	36.0	32.0	36.0
38	33.91760155859581	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	12.0
22	61.0
23	155.0
24	507.0
25	1255.0
26	2597.0
27	5411.0
28	9463.0
29	15176.0
30	23214.0
31	33704.0
32	48711.0
33	77534.0
34	188001.0
35	422634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.650887545391335	17.99223035617708	11.947940006180948	26.408942092250637
2	15.43772738575488	20.37666065535381	37.50938513201953	26.676226826871773
3	18.33897044799973	24.499841871087515	28.552408267124395	28.60877941378836
4	11.881275363998272	15.981883979247694	35.96322235339277	36.173618303361266
5	13.683840774856748	37.52017353160228	33.445995289925484	15.349990403615482
6	34.495562124820594	35.747558353863965	16.4511000836515	13.30577943766394
7	30.105524144291167	30.584136855472238	20.59881511667769	18.711523883558897
8	28.173545029749437	33.64903508973116	18.992925077768614	19.18449480275078
9	27.461259984524517	13.92443569404894	18.18550543392318	40.42879888750336
10	15.498333596883748	27.37307029794877	32.119391959016504	25.009204146150978
11	37.63153352060174	21.163301348018653	21.900021849321767	19.305143282057845
12	24.576430768339378	24.059191053986066	29.092410276714194	22.271967900960362
13	30.183502406945134	19.646055941557343	24.973081807164345	25.197359844333178
14	23.13220784174555	19.696585622581292	25.560633384755405	31.61057315091775
15	25.12016590257811	27.447799352516423	22.781306506944393	24.65072823796108
16	25.370999398868715	25.891738428222748	24.4385216515901	24.298740521318432
17	23.586677578768718	26.107807705585696	25.80820773552155	24.49730698012404
18	24.509498598567426	24.80801218679008	27.16787496469259	23.514614249949904
19	25.10278379311427	25.19476412236039	26.067732286544075	23.63471979798126
20	25.337804398156532	24.340747285856033	26.273903418240106	24.04754489774733
21	26.202202216701792	24.507446543977945	24.987023772448875	24.303327466871387
22	25.182602901608693	24.84292710248335	25.620656730686825	24.353813265221135
23	23.858663965998623	24.71062977631371	26.299404782741476	25.131301474946195
24	24.306828030582857	25.305575070192337	25.950040920382694	24.43755597884211
25	24.653322646529123	24.759667614637703	25.91292507809897	24.674084660734202
26	24.052011134206786	25.53745723880363	26.305649909830308	24.10488171715928
27	24.835714527133053	25.36792220521561	25.59485584885254	24.201507418798794
28	23.931422749801435	25.04943037378778	26.2017193803278	24.81742749608299
29	23.98646609643691	25.24353059613393	26.37481622040515	24.395187087024013
30	24.16282208203873	25.16700103085566	26.590161243207096	24.080015643898516
31	24.51517192596187	25.051844555657755	25.665650296099408	24.767333222280968
32	24.021230315364576	25.439079327602066	25.607106385752466	24.93258397128089
33	23.649204889201123	25.232304650438536	26.22054999891362	24.897940461446723
34	24.36923463192176	25.195971213295383	26.402941439190382	24.03185271559248
35	24.91725391640654	25.308592797529812	26.087890705158383	23.68626258090527
36	24.114538444639187	25.775978407557353	25.808690571895543	24.300792575907913
37	24.93705020774035	25.504382947184943	25.408660636040352	24.149906209034352
38	24.03912427861141	25.71143007856964	25.736537600324468	24.51290804249448
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	49.0
2	66.0
3	66.0
4	266.0
5	466.0
6	466.0
7	687.5
8	909.0
9	1003.5
10	1098.0
11	1098.0
12	1419.0
13	1740.0
14	2147.5
15	2555.0
16	2555.0
17	3828.5
18	5102.0
19	5102.0
20	5784.5
21	6467.0
22	6706.0
23	6945.0
24	6945.0
25	7923.0
26	8901.0
27	8901.0
28	11569.0
29	14237.0
30	17862.0
31	21487.0
32	21487.0
33	26362.5
34	31238.0
35	31238.0
36	34394.0
37	37550.0
38	42220.0
39	46890.0
40	46890.0
41	49681.0
42	52472.0
43	58281.5
44	64091.0
45	64091.0
46	69357.0
47	74623.0
48	74623.0
49	76292.5
50	77962.0
51	76471.0
52	74980.0
53	74980.0
54	71852.0
55	68724.0
56	68724.0
57	65911.0
58	63098.0
59	56976.5
60	50855.0
61	50855.0
62	46656.0
63	42457.0
64	35442.5
65	28428.0
66	28428.0
67	23531.0
68	18634.0
69	18634.0
70	14895.0
71	11156.0
72	8757.5
73	6359.0
74	6359.0
75	4753.0
76	3147.0
77	3147.0
78	2861.0
79	2575.0
80	1990.0
81	1405.0
82	1405.0
83	1277.0
84	1149.0
85	1149.0
86	751.5
87	354.0
88	281.5
89	209.0
90	209.0
91	128.5
92	48.0
93	30.5
94	13.0
95	13.0
96	11.5
97	10.0
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01038098204090107
2	0.0
3	0.0
4	0.0
5	1.2070909349884964E-4
6	1.2070909349884964E-4
7	2.4141818699769928E-4
8	0.0022934727764781435
9	0.003741981898464339
10	8.449636544919474E-4
11	0.004466236459457437
12	2.4141818699769928E-4
13	2.4141818699769928E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.2070909349884964E-4
23	1.2070909349884964E-4
24	0.0
25	2.4141818699769928E-4
26	0.0
27	2.4141818699769928E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.2070909349884964E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	828438.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.971604469706975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.94642869192077	45.376543581764636
2	14.39208530634194	16.974487243597565
3	4.378904937970429	7.746931500373167
4	1.7773925787580653	4.192627685676525
5	0.8241171532398152	2.429975539877964
6	0.43062916403496365	1.5236935640754263
7	0.2560008313255076	1.0567745838190803
8	0.17367175142070354	0.8193361465874399
9	0.1171980489871177	0.6220221290540683
>10	0.5624672853263308	6.211403574914027
>50	0.06952503078013567	2.8534821472178264
>100	0.06705409680935547	7.506839120716316
>500	0.0032909986071549048	1.2385653784369635
>1k	0.0012341244776830891	1.4473178038889283
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3467	0.4184984271605117	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2551	0.30792889751556546	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1707	0.20605042260253634	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1604	0.19361738597215483	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1540	0.18589200398822844	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1063	0.12831376638927716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4141818699769928E-4
2	0.0	0.0	0.0	0.0	4.8283637399539856E-4
3	0.0	0.0	0.0	0.0	4.8283637399539856E-4
4	0.0	0.0	0.0	0.0	4.8283637399539856E-4
5	0.0	0.0	0.0	0.0	4.8283637399539856E-4
6	0.0	0.0	0.0	1.2070909349884964E-4	4.8283637399539856E-4
7	0.0	0.0	0.0	1.2070909349884964E-4	4.8283637399539856E-4
8	0.0	0.0	0.0	1.2070909349884964E-4	4.8283637399539856E-4
9	0.0	0.0	0.0	1.2070909349884964E-4	4.8283637399539856E-4
10	0.0	0.0	0.0	1.2070909349884964E-4	4.8283637399539856E-4
11	0.0	0.0	0.0	1.2070909349884964E-4	4.8283637399539856E-4
12	0.0	0.0	0.0	1.2070909349884964E-4	7.242545609930978E-4
13	0.0	0.0	0.0	1.2070909349884964E-4	8.449636544919475E-4
14	0.0	0.0	0.0	1.2070909349884964E-4	9.656727479907971E-4
15	0.0	0.0	0.0	1.2070909349884964E-4	0.0010863818414896468
16	0.0	0.0	0.0	1.2070909349884964E-4	0.0014485091219861956
17	0.0	0.0	0.0	1.2070909349884964E-4	0.0014485091219861956
18	0.0	0.0	0.0	1.2070909349884964E-4	0.0015692182154850454
19	0.0	0.0	0.0	1.2070909349884964E-4	0.0015692182154850454
20	0.0	0.0	0.0	2.4141818699769928E-4	0.0019313454959815942
21	0.0	0.0	0.0	4.8283637399539856E-4	0.0019313454959815942
22	0.0	0.0	0.0	4.8283637399539856E-4	0.0019313454959815942
23	0.0	0.0	0.0	0.001327800028487346	0.0021727636829792936
24	0.0	0.0	0.0	0.0028970182439723912	0.0021727636829792936
25	0.0	0.0	0.0	0.00337985461796779	0.0021727636829792936
26	0.0	0.0	0.0	0.004104109178960888	0.002293472776478143
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTCGT	20	0.0037534705	31.999035	18
CGACCCA	95	3.786954E-8	18.525757	17
TCTAGCG	140	1.8189894E-12	18.285162	28
GATATAC	150	0.0	18.134975	1
AGCGTAT	125	1.4733814E-10	17.921623	8
CTATACT	90	4.3589534E-7	17.779387	4
AATAGCG	145	3.6379788E-12	17.65677	5
CTAGCGG	145	3.6379788E-12	17.65464	29
CGGTCGC	55	0.0013644858	17.45402	10
TAGCGTA	130	2.582965E-10	17.232328	7
CGAACCC	75	3.2327676E-5	17.06615	10
CGACGAC	95	7.68845E-7	16.841597	14
ACGACCC	105	1.1882912E-7	16.7614	16
ATAGCGT	135	4.4201443E-10	16.594095	6
CGTTTAT	165	1.8189894E-12	16.48435	31
GTCCTAT	170	3.6379788E-12	16.00145	1
GTCTTAC	80	5.6134697E-5	16.00145	1
TTAGGCA	60	0.0024396584	16.001448	4
TAGGACG	280	0.0	16.001448	4
TATAATA	110	2.0164771E-7	16.000483	2
>>END_MODULE