FastQCFastQC Report
Thu 2 Feb 2017
SRR4062999_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062999_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718330
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT52150.3034923443110462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34840.20275500049466633No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23310.135654967322924No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22440.13059191191447508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20470.1191272921964931No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20320.11825435160882951No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20040.11662486251185744No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19810.11528635361077325No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19460.11324949223955817No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18980.11045608235903465No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18670.10865200514452987No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18420.10719710416509053No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17690.10294879330512766No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17610.10248322499170706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT5450.018.20132329
CATCGTT5700.017.68268228
CGTACTA753.239214E-517.0641983
GCATCGT5600.016.85470627
CGGCATC6050.016.66068825
TACCGTC5200.016.6144317
ATACCGT5200.016.304866
ACCGTCG5300.016.3009538
CGTTTAT6100.016.26183731
GGCATCG6000.015.99954826
GTATTAC1551.8735591E-1015.4965141
CCGTCGT5400.015.40831859
TCGTTTA6350.015.36964530
CGTCGTA5350.015.25323810
GTATAGG1052.1678734E-615.2505371
GTCTTAC1603.0195224E-1015.0122481
GTCCTAT5900.014.9274321
GTCGTAG5550.014.70357211
GTCTATA1205.370912E-714.6786421
TAGACAG2650.014.4876285
Produced by FastQC (version 0.11.5)