##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062999_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1718330
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223580453114362	32.0	32.0	32.0	32.0	32.0
2	30.81671681225376	32.0	32.0	32.0	32.0	32.0
3	30.866789266322535	32.0	32.0	32.0	32.0	32.0
4	30.936652447434426	32.0	32.0	32.0	32.0	32.0
5	30.850723085786782	32.0	32.0	32.0	32.0	32.0
6	34.51728829735848	36.0	36.0	36.0	32.0	36.0
7	34.42846310080136	36.0	36.0	36.0	32.0	36.0
8	34.41353232498996	36.0	36.0	36.0	32.0	36.0
9	34.54935256906415	36.0	36.0	36.0	32.0	36.0
10	34.252281575715955	36.0	36.0	36.0	32.0	36.0
11	34.556853456553746	36.0	36.0	36.0	32.0	36.0
12	34.35706819993831	36.0	36.0	36.0	32.0	36.0
13	34.4487898133653	36.0	36.0	36.0	32.0	36.0
14	34.34168407698172	36.0	36.0	36.0	32.0	36.0
15	34.28731500934047	36.0	36.0	36.0	32.0	36.0
16	34.30487682808308	36.0	36.0	36.0	32.0	36.0
17	34.22974283170287	36.0	36.0	36.0	32.0	36.0
18	34.25335354675761	36.0	36.0	36.0	32.0	36.0
19	34.249035982611026	36.0	36.0	36.0	32.0	36.0
20	34.227865427479	36.0	36.0	36.0	32.0	36.0
21	34.203439385915395	36.0	36.0	36.0	32.0	36.0
22	34.184437797163525	36.0	36.0	36.0	32.0	36.0
23	34.128963004777894	36.0	36.0	36.0	32.0	36.0
24	34.11123416340284	36.0	36.0	36.0	32.0	36.0
25	34.08863722335058	36.0	36.0	36.0	32.0	36.0
26	34.039229950009606	36.0	36.0	36.0	32.0	36.0
27	34.04998457804962	36.0	36.0	36.0	32.0	36.0
28	34.02460994104741	36.0	36.0	36.0	32.0	36.0
29	33.992985049437536	36.0	36.0	36.0	32.0	36.0
30	33.97118830492397	36.0	36.0	36.0	32.0	36.0
31	33.98682208888863	36.0	36.0	36.0	32.0	36.0
32	33.93561539401628	36.0	36.0	36.0	32.0	36.0
33	33.889206380613736	36.0	36.0	36.0	32.0	36.0
34	33.906850255771595	36.0	36.0	36.0	32.0	36.0
35	33.8559368689367	36.0	36.0	36.0	32.0	36.0
36	33.81019536410352	36.0	36.0	36.0	32.0	36.0
37	33.80757305057818	36.0	36.0	36.0	32.0	36.0
38	33.202759656177804	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	3.0
8	3.0
9	8.0
10	19.0
11	22.0
12	14.0
13	6.0
14	244.0
15	539.0
16	653.0
17	764.0
18	999.0
19	1215.0
20	1554.0
21	2317.0
22	3477.0
23	5248.0
24	7846.0
25	11795.0
26	17225.0
27	24527.0
28	34109.0
29	46339.0
30	61966.0
31	82793.0
32	113240.0
33	168175.0
34	370596.0
35	762632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.216469196729	17.48528079380293	11.642229337031221	25.656020672436846
2	16.38824224031415	20.322673433476844	37.867313535211906	25.4217707909971
3	18.441684069368684	24.973226612281767	28.76381448603244	27.821274832317112
4	12.357946526728197	15.976159866936626	36.7908238417626	34.87506976457257
5	14.453651720539803	36.82971867403585	33.56904710221046	15.147582503213883
6	32.946134108662925	36.27886026084735	17.445497732382094	13.329507898107632
7	29.482305193010188	31.032823167158323	21.483487737214467	18.001383902617018
8	27.89917844316814	32.565123088034085	20.042622952727747	19.49307551607003
9	27.91356238571726	14.252708740949041	18.780080147771727	39.05364872556197
10	15.935497500176044	26.549055735220602	31.764553887195703	25.750892877407654
11	36.954217882768404	21.532674034840433	22.53500716700489	18.978100915386275
12	24.99364187180966	24.227931184392702	28.74502480106479	22.03340214273285
13	29.363786849445194	19.77553909265735	25.25697409654543	25.603699961352028
14	23.930530536939116	20.134794970215317	25.351290475594602	30.583384017250964
15	25.101562186210824	26.891350144041056	23.01292323883689	24.99416443091123
16	25.50678379339506	25.845638798723503	24.078494231258354	24.56908317662308
17	23.88142844669519	25.77863150848395	25.438633254751302	24.90130679006956
18	24.851780352654217	24.721122412812743	26.558656103064383	23.86844113146866
19	25.436344535981075	25.19597600970786	25.850034483862427	23.51764497044864
20	25.684311242306006	24.16896749201501	25.497839629757234	24.648881635921743
21	27.262685126931295	24.263755424967684	24.531941034085992	23.941618414015025
22	25.86966112171007	24.441944409200804	25.08575845158707	24.602636017502057
23	24.216690199620757	24.278558782137615	25.596190804338136	25.908560213903492
24	24.556492849231145	25.440334981115402	25.399184083042165	24.603988086611288
25	24.831914357374725	24.53389739252618	25.492557433747976	25.141630816351118
26	24.432677408727216	25.489906180623546	25.96651187265059	24.11090453799865
27	25.32290131604985	24.99598410403615	24.832205390380008	24.848909189533995
28	24.240129762120237	24.77696408087202	26.079881458787895	24.903024698219845
29	24.286281352692544	25.100488093339557	25.916324527941686	24.696906026026213
30	24.304488184897924	25.26557281260712	26.225202485993155	24.2047365165018
31	24.896302267178438	25.151974578268728	24.798235377994025	25.153487776558812
32	24.331962716905142	25.198797582360662	24.883992305923915	25.585247394810285
33	24.101143998547304	24.783841544328197	25.476025850549185	25.638988606575314
34	24.990600223732137	24.86703536294777	25.76621272246622	24.376151690853874
35	25.80573437481811	24.64343503719311	25.498236382896987	24.05259420509179
36	24.429470120811246	25.5413689457595	25.132766645014236	24.89639428841502
37	25.409520727612623	25.433383367274544	24.962416342532478	24.194679562580355
38	24.32133570166474	25.2080049911886	25.631813705748012	24.838845601398653
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	759.0
1	598.5
2	438.0
3	438.0
4	933.0
5	1428.0
6	1428.0
7	1696.0
8	1964.0
9	1883.5
10	1803.0
11	1803.0
12	2301.5
13	2800.0
14	3667.0
15	4534.0
16	4534.0
17	6717.0
18	8900.0
19	8900.0
20	10674.0
21	12448.0
22	13520.5
23	14593.0
24	14593.0
25	16739.0
26	18885.0
27	18885.0
28	24260.5
29	29636.0
30	35704.0
31	41772.0
32	41772.0
33	52078.5
34	62385.0
35	62385.0
36	68921.5
37	75458.0
38	85934.0
39	96410.0
40	96410.0
41	102827.5
42	109245.0
43	123347.0
44	137449.0
45	137449.0
46	142335.0
47	147221.0
48	147221.0
49	153673.5
50	160126.0
51	158808.0
52	157490.0
53	157490.0
54	149793.0
55	142096.0
56	142096.0
57	137093.5
58	132091.0
59	117411.0
60	102731.0
61	102731.0
62	97559.5
63	92388.0
64	76094.0
65	59800.0
66	59800.0
67	49636.5
68	39473.0
69	39473.0
70	31960.5
71	24448.0
72	19162.5
73	13877.0
74	13877.0
75	10570.5
76	7264.0
77	7264.0
78	6962.0
79	6660.0
80	5171.5
81	3683.0
82	3683.0
83	3776.0
84	3869.0
85	3869.0
86	2620.5
87	1372.0
88	1170.0
89	968.0
90	968.0
91	700.5
92	433.0
93	378.5
94	324.0
95	324.0
96	296.5
97	269.0
98	269.0
99	554.5
100	840.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11842893972636222
2	0.054005924356788276
3	0.011988384070580158
4	0.0029679979980562522
5	1.7458811753272071E-4
6	4.0737227424301505E-4
7	5.8196039177573576E-5
8	4.0737227424301505E-4
9	2.327841567102943E-4
10	5.237643525981622E-4
11	0.0022696455279253695
12	0.0033171742331216934
13	0.015014578107813982
14	0.00960234646429964
15	0.02426774833704818
16	0.011115443482916553
17	0.022114494887477958
18	0.005994192035290079
19	0.007856465288972433
20	0.006168780152822799
21	0.006808936583776108
22	0.0076818771714397115
23	0.010009718738542655
24	0.015305558303701849
25	0.017866184027515088
26	0.020252221633795604
27	0.009194974190056625
28	0.00634336827035552
29	0.012570344462355894
30	0.003666350468187135
31	0.00669254450542096
32	0.0072745048971966965
33	0.008496621719925742
34	0.011871991992225009
35	0.015712930577944866
36	0.016178498891365453
37	0.009485954385944493
38	0.0059359959961125044
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1718330.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.081621613709565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4279369562405	43.750195689812166
2	12.961330195716043	14.278621709015582
3	3.7042107535291775	6.121018053099843
4	1.4623258301660538	3.2218911221264115
5	0.691849836667637	1.9054105458416772
6	0.39732074571537074	1.313104258486258
7	0.2538575591179975	0.9788020210582211
8	0.16734170676075497	0.7373962057590598
9	0.12596797058771425	0.6244668082223435
>10	0.6460604865600653	6.3197103190558215
>50	0.06901443405336963	2.6916579665018516
>100	0.07626077555694429	9.274328539306135
>500	0.01175657163755265	4.468648487984888
>1k	0.004660262631101952	4.010506568899998
>5k	1.0591505979777161E-4	0.3042417048297086
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5215	0.3034923443110462	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3484	0.20275500049466633	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2331	0.135654967322924	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2244	0.13059191191447508	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2047	0.1191272921964931	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2032	0.11825435160882951	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2004	0.11662486251185744	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1981	0.11528635361077325	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1946	0.11324949223955817	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1898	0.11045608235903465	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1867	0.10865200514452987	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1842	0.10719710416509053	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1769	0.10294879330512766	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1761	0.10248322499170706	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.8196039177573576E-5
2	0.0	0.0	0.0	0.0	5.8196039177573576E-5
3	0.0	0.0	0.0	0.0	5.8196039177573576E-5
4	0.0	0.0	0.0	0.0	5.8196039177573576E-5
5	0.0	0.0	0.0	0.0	5.8196039177573576E-5
6	0.0	0.0	0.0	0.0	5.8196039177573576E-5
7	0.0	0.0	0.0	5.8196039177573576E-5	5.8196039177573576E-5
8	0.0	0.0	0.0	5.8196039177573576E-5	5.8196039177573576E-5
9	0.0	0.0	0.0	5.8196039177573576E-5	5.8196039177573576E-5
10	0.0	0.0	0.0	5.8196039177573576E-5	5.8196039177573576E-5
11	0.0	0.0	0.0	5.8196039177573576E-5	5.8196039177573576E-5
12	0.0	0.0	0.0	5.8196039177573576E-5	1.7458811753272071E-4
13	0.0	0.0	0.0	5.8196039177573576E-5	1.7458811753272071E-4
14	0.0	0.0	0.0	5.8196039177573576E-5	1.7458811753272071E-4
15	0.0	0.0	0.0	1.1639207835514715E-4	2.327841567102943E-4
16	0.0	0.0	0.0	1.1639207835514715E-4	2.327841567102943E-4
17	0.0	0.0	0.0	1.1639207835514715E-4	2.327841567102943E-4
18	0.0	0.0	0.0	2.327841567102943E-4	2.9098019588786787E-4
19	0.0	0.0	0.0	2.327841567102943E-4	2.9098019588786787E-4
20	0.0	0.0	0.0	2.9098019588786787E-4	3.4917623506544143E-4
21	0.0	0.0	0.0	5.237643525981622E-4	3.4917623506544143E-4
22	0.0	0.0	0.0	8.147445484860301E-4	4.0737227424301505E-4
23	0.0	0.0	0.0	0.0011639207835514715	4.0737227424301505E-4
24	0.0	0.0	0.0	0.0020368613712150752	4.0737227424301505E-4
25	0.0	0.0	0.0	0.0024442336454580903	4.0737227424301505E-4
26	0.0	0.0	0.0	0.003666350468187135	4.0737227424301505E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	545	0.0	18.201323	29
CATCGTT	570	0.0	17.682682	28
CGTACTA	75	3.239214E-5	17.064198	3
GCATCGT	560	0.0	16.854706	27
CGGCATC	605	0.0	16.660688	25
TACCGTC	520	0.0	16.614431	7
ATACCGT	520	0.0	16.30486	6
ACCGTCG	530	0.0	16.300953	8
CGTTTAT	610	0.0	16.261837	31
GGCATCG	600	0.0	15.999548	26
GTATTAC	155	1.8735591E-10	15.496514	1
CCGTCGT	540	0.0	15.4083185	9
TCGTTTA	635	0.0	15.369645	30
CGTCGTA	535	0.0	15.253238	10
GTATAGG	105	2.1678734E-6	15.250537	1
GTCTTAC	160	3.0195224E-10	15.012248	1
GTCCTAT	590	0.0	14.927432	1
GTCGTAG	555	0.0	14.703572	11
GTCTATA	120	5.370912E-7	14.678642	1
TAGACAG	265	0.0	14.487628	5
>>END_MODULE