FastQCFastQC Report
Thu 2 Feb 2017
SRR4062999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718330
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT61030.35517042710073154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT44550.2592633545360903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT27700.1612030285218788No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24430.14217292371081225No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22690.13204681289391446No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21480.12500509215342806No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20430.11889450803978283No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19780.11511176549324054No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19290.11226015957353941No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19170.11156180710340854No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19090.11109623878998796No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA18910.11004871008479163No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18530.10783726059604383No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.10603318338153905No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17980.1046364784412773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA601.1386704E-418.66651332
ACCGTCG4350.017.655548
TACCGTC4350.017.655547
CGTCGTA4450.017.25878710
ATACCGT4500.017.0670246
GTATAAT1800.016.8897341
CCGTCGT4500.016.711469
GTCGTAG4600.016.69600111
ATCGTTT4800.015.99986929
AACGAAT600.002442715.99986931
CATCGTT4800.015.99986928
CGTTTAT5050.015.841454531
TCGTTTA5150.015.22317730
GTATAGC2350.014.9794721
ATAACGA1401.2798409E-814.85702112
ATAGCAC650.00416292114.769113
TAAACGC4550.014.41746428
TCCGAAA5400.014.22210621
CGCTTCG4500.013.86655432
GTCCTAT5200.013.8468461
Produced by FastQC (version 0.11.5)