##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062999_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1718330
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204387399393596	32.0	32.0	32.0	32.0	32.0
2	31.316030680951854	32.0	32.0	32.0	32.0	32.0
3	31.397193205030465	32.0	32.0	32.0	32.0	32.0
4	31.48959163839309	32.0	32.0	32.0	32.0	32.0
5	31.421593640336837	32.0	32.0	32.0	32.0	32.0
6	34.9475799177108	36.0	36.0	36.0	36.0	36.0
7	34.96837394446934	36.0	36.0	36.0	36.0	36.0
8	34.91540099980795	36.0	36.0	36.0	36.0	36.0
9	35.01336879411987	36.0	36.0	36.0	36.0	36.0
10	34.87267404980417	36.0	36.0	36.0	32.0	36.0
11	35.04676109943957	36.0	36.0	36.0	36.0	36.0
12	34.93957563448232	36.0	36.0	36.0	36.0	36.0
13	34.9885155936287	36.0	36.0	36.0	36.0	36.0
14	34.93461733194439	36.0	36.0	36.0	32.0	36.0
15	34.91509547060227	36.0	36.0	36.0	32.0	36.0
16	34.922491605221346	36.0	36.0	36.0	32.0	36.0
17	34.88884149144809	36.0	36.0	36.0	32.0	36.0
18	34.887624030308494	36.0	36.0	36.0	32.0	36.0
19	34.88080811020002	36.0	36.0	36.0	32.0	36.0
20	34.861483533430714	36.0	36.0	36.0	32.0	36.0
21	34.85483929163781	36.0	36.0	36.0	32.0	36.0
22	34.833952151216586	36.0	36.0	36.0	32.0	36.0
23	34.77469752608638	36.0	36.0	36.0	32.0	36.0
24	34.76082708210879	36.0	36.0	36.0	32.0	36.0
25	34.734301909412046	36.0	36.0	36.0	32.0	36.0
26	34.67474408291772	36.0	36.0	36.0	32.0	36.0
27	34.65912601188363	36.0	36.0	36.0	32.0	36.0
28	34.62604447341314	36.0	36.0	36.0	32.0	36.0
29	34.59670610418255	36.0	36.0	36.0	32.0	36.0
30	34.572415659390224	36.0	36.0	36.0	32.0	36.0
31	34.56579004032986	36.0	36.0	36.0	32.0	36.0
32	34.52875524491803	36.0	36.0	36.0	32.0	36.0
33	34.48542596590876	36.0	36.0	36.0	32.0	36.0
34	34.48540617925544	36.0	36.0	36.0	32.0	36.0
35	34.44288989891348	36.0	36.0	36.0	32.0	36.0
36	34.40796296404067	36.0	36.0	36.0	32.0	36.0
37	34.403559851716494	36.0	36.0	36.0	32.0	36.0
38	33.975737489306475	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	30.0
22	105.0
23	403.0
24	1150.0
25	2778.0
26	5762.0
27	11233.0
28	19906.0
29	31340.0
30	47537.0
31	67626.0
32	96987.0
33	154524.0
34	379907.0
35	899032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.56877957361612	18.729054750112038	12.349709283714649	26.3524563925572
2	15.454307379839726	21.29585120436703	37.146357218927676	26.103484196865562
3	17.486338479803067	25.58251325414792	29.165876170467836	27.765272095581174
4	11.861195139921064	16.874737389761897	36.67121372777925	34.59285374253779
5	13.887611809140271	37.38455360728149	33.32223728853015	15.405597295048098
6	32.995679521022765	36.69316917375495	17.104301390654168	13.206849914568114
7	29.186147881479723	31.218246638290147	21.477348250211545	18.11825723001859
8	27.708008517805272	32.895105171848876	19.891892080640964	19.504994229704884
9	27.724303294311415	13.973863170116369	18.73511943733941	39.56671409823281
10	15.661052656082685	26.911634619861257	31.726453745518878	25.700858978537177
11	36.97707091035435	21.419534015343483	22.56447533368175	19.03891974062042
12	24.65240819100949	24.325636099848516	28.825895946221987	22.196059762920008
13	29.42672112276716	19.70091821924361	25.305500818820175	25.566859839169055
14	23.859386730139146	20.050281377849423	25.446218130394044	30.644113761617387
15	25.15186256423388	26.97590102017657	22.818085001134822	25.054151414454733
16	25.46444830995694	25.937349599523724	23.941980843016676	24.65622124750266
17	23.814808564129127	25.816810507876838	25.446974678903356	24.921406249090687
18	24.82771062601479	24.660978973771044	26.647558967136696	23.863751433077464
19	25.353337251866638	25.201736569809057	25.95904162762682	23.48588455069748
20	25.659273829823142	24.029377360576838	25.628604517176562	24.682744292423457
21	27.19626287879846	24.255846380900504	24.56568245410655	23.98220828619449
22	25.679540995932676	24.447064560977555	25.243303232384484	24.630091210705285
23	24.115563635771093	24.123361923174066	25.84928587474088	25.91178856631396
24	24.547147521139713	25.290892901829103	25.617023505380228	24.544936071650962
25	24.69305936174039	24.420236171303632	25.71579714943995	25.17090731751603
26	24.396012407395553	25.349787293476805	26.088062246483506	24.166138052644136
27	25.3043943829183	24.829805683425185	25.008583915778694	24.85721601787782
28	24.214615353279058	24.640726752137248	26.21027392875641	24.934383965827287
29	24.36109478388901	24.915877625368818	25.999371482776883	24.72365610796529
30	24.338631112766464	24.986934989204634	26.378693266136306	24.295740631892592
31	24.857390605995356	24.826255725035352	25.06654717079956	25.249806498169736
32	24.422607997299703	25.08255108157339	25.079524887536152	25.415316033590752
33	24.131685997451015	24.483131878044382	25.75075800341029	25.63442412109432
34	24.8121140875152	24.69711871410032	26.042960316120883	24.447806882263592
35	25.821117014775975	24.47905815530195	25.682261265298283	24.017563564623792
36	24.51269546594659	25.230252629006074	25.41566520982582	24.841386695221523
37	25.41677093457019	25.122939132762625	25.106353261597015	24.353936671070166
38	24.345570609586407	24.888365382880696	25.818455022292007	24.947608985240894
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	424.0
1	340.0
2	256.0
3	256.0
4	815.0
5	1374.0
6	1374.0
7	1691.5
8	2009.0
9	1981.0
10	1953.0
11	1953.0
12	2417.0
13	2881.0
14	3767.0
15	4653.0
16	4653.0
17	7169.5
18	9686.0
19	9686.0
20	11369.5
21	13053.0
22	14022.0
23	14991.0
24	14991.0
25	16933.5
26	18876.0
27	18876.0
28	24174.0
29	29472.0
30	35419.5
31	41367.0
32	41367.0
33	52253.5
34	63140.0
35	63140.0
36	69499.0
37	75858.0
38	86311.0
39	96764.0
40	96764.0
41	102889.0
42	109014.0
43	123499.0
44	137984.0
45	137984.0
46	142356.5
47	146729.0
48	146729.0
49	153759.5
50	160790.0
51	159181.5
52	157573.0
53	157573.0
54	149943.0
55	142313.0
56	142313.0
57	137552.0
58	132791.0
59	117986.0
60	103181.0
61	103181.0
62	98209.5
63	93238.0
64	76011.0
65	58784.0
66	58784.0
67	49196.5
68	39609.0
69	39609.0
70	32035.0
71	24461.0
72	19025.5
73	13590.0
74	13590.0
75	10214.0
76	6838.0
77	6838.0
78	6573.5
79	6309.0
80	4895.0
81	3481.0
82	3481.0
83	3341.5
84	3202.0
85	3202.0
86	2040.0
87	878.0
88	751.5
89	625.0
90	625.0
91	366.5
92	108.0
93	73.5
94	39.0
95	39.0
96	28.5
97	18.0
98	18.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009311366268411772
2	0.0
3	0.0
4	2.327841567102943E-4
5	0.0
6	1.1639207835514715E-4
7	4.655683134205886E-4
8	0.002735213841345958
9	0.0037827425465422823
10	5.819603917757357E-4
11	0.004422898977495591
12	4.0737227424301505E-4
13	2.327841567102943E-4
14	0.0
15	0.0
16	5.8196039177573576E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1639207835514715E-4
22	5.8196039177573576E-5
23	2.327841567102943E-4
24	0.0
25	5.8196039177573576E-5
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.8196039177573576E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1718330.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.12532765677275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.02487349332215	41.45096969710981
2	13.743586756140225	14.602650991984637
3	4.042598530818401	6.442931146035469
4	1.5697615968786376	3.335763967087858
5	0.7539775286067373	2.002765162653833
6	0.4324280423213114	1.3783728861777877
7	0.26443885097386566	0.9833880422216978
8	0.18363050697732405	0.7804344680759707
9	0.13436437463243742	0.6424336284971041
>10	0.6798672772358434	6.483404057655916
>50	0.07021942684449482	2.6492546222452185
>100	0.08231613377657411	9.729627200247904
>500	0.012325140643350066	4.600510920964256
>1k	0.005502294930066994	4.560698132660787
>5k	1.1004589860133987E-4	0.35679507638190483
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6103	0.35517042710073154	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4455	0.2592633545360903	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2770	0.1612030285218788	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2443	0.14217292371081225	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2269	0.13204681289391446	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2148	0.12500509215342806	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2043	0.11889450803978283	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1978	0.11511176549324054	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1929	0.11226015957353941	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1917	0.11156180710340854	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1909	0.11109623878998796	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1891	0.11004871008479163	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1853	0.10783726059604383	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1822	0.10603318338153905	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1798	0.1046364784412773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1639207835514715E-4
2	0.0	0.0	0.0	0.0	1.1639207835514715E-4
3	0.0	0.0	0.0	0.0	1.1639207835514715E-4
4	0.0	0.0	0.0	0.0	1.1639207835514715E-4
5	0.0	0.0	0.0	0.0	1.7458811753272071E-4
6	0.0	0.0	0.0	0.0	2.327841567102943E-4
7	0.0	0.0	0.0	0.0	2.327841567102943E-4
8	0.0	0.0	0.0	0.0	2.327841567102943E-4
9	0.0	0.0	0.0	0.0	2.327841567102943E-4
10	0.0	0.0	0.0	0.0	2.327841567102943E-4
11	0.0	0.0	0.0	0.0	2.327841567102943E-4
12	0.0	0.0	0.0	0.0	4.0737227424301505E-4
13	0.0	0.0	0.0	0.0	5.819603917757357E-4
14	0.0	0.0	0.0	0.0	5.819603917757357E-4
15	0.0	0.0	0.0	0.0	5.819603917757357E-4
16	0.0	0.0	0.0	0.0	5.819603917757357E-4
17	0.0	0.0	0.0	0.0	5.819603917757357E-4
18	0.0	0.0	0.0	1.1639207835514715E-4	6.401564309533093E-4
19	0.0	0.0	0.0	1.1639207835514715E-4	6.401564309533093E-4
20	0.0	0.0	0.0	2.327841567102943E-4	6.983524701308829E-4
21	0.0	0.0	0.0	4.655683134205886E-4	6.983524701308829E-4
22	0.0	0.0	0.0	7.565485093084564E-4	7.565485093084564E-4
23	0.0	0.0	0.0	0.0010475287051963243	8.147445484860301E-4
24	0.0	0.0	0.0	0.0019204692928599279	8.147445484860301E-4
25	0.0	0.0	0.0	0.0023860376062805164	8.147445484860301E-4
26	0.0	0.0	0.0	0.0034335663114768407	8.147445484860301E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	60	1.1386704E-4	18.666513	32
ACCGTCG	435	0.0	17.65554	8
TACCGTC	435	0.0	17.65554	7
CGTCGTA	445	0.0	17.258787	10
ATACCGT	450	0.0	17.067024	6
GTATAAT	180	0.0	16.889734	1
CCGTCGT	450	0.0	16.71146	9
GTCGTAG	460	0.0	16.696001	11
ATCGTTT	480	0.0	15.999869	29
AACGAAT	60	0.0024427	15.999869	31
CATCGTT	480	0.0	15.999869	28
CGTTTAT	505	0.0	15.8414545	31
TCGTTTA	515	0.0	15.223177	30
GTATAGC	235	0.0	14.979472	1
ATAACGA	140	1.2798409E-8	14.857021	12
ATAGCAC	65	0.004162921	14.76911	3
TAAACGC	455	0.0	14.417464	28
TCCGAAA	540	0.0	14.222106	21
CGCTTCG	450	0.0	13.866554	32
GTCCTAT	520	0.0	13.846846	1
>>END_MODULE