FastQCFastQC Report
Thu 2 Feb 2017
SRR4062998_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062998_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902360
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25220.27948933906644796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16650.18451615763110066No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13110.1452856952879117No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12900.14295846447094288No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12530.13885810541247395No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12120.13431446429363003No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11490.12733277184272354No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11480.12722195132762978No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11040.12234584866350458No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10950.1213484640276608No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10930.1211268229974733No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10620.11769138702956693No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10430.11558579724278559No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9960.11037723303337914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9720.10771754067112904No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9710.10760672015603528No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9670.10716343809566027No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9290.1029522585220976No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC9190.10184405337116008No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9160.10151159182587881No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT9100.10084666873531628No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT9050.1002925661598475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC2000.019.19750630
CTAGCGG2050.018.73031229
GTCGTAA701.7885655E-518.28333930
TCTAGCG2150.017.85913728
ATTCTAC904.314552E-717.7971671
ACGAACG1352.3646862E-1117.7774415
CGTCGTA2550.017.56829510
GAATTAT550.00135440717.4735811
GAGCGTT550.001365525317.4522787
CGAACGA1404.0017767E-1117.1425316
GATAACG1404.0017767E-1117.1425311
TACCGTC2900.017.1012277
CCGTCGT2650.016.9053389
ATACCGT2800.016.568366
ATAACGA1456.91216E-1116.55140912
ACCGTCG2750.016.2887948
GATATAG600.002423661516.017451
CCTATAC600.002438961316.0023573
CGTGCGC805.619626E-515.99969510
CCGATAA1407.421477E-1015.9996959
Produced by FastQC (version 0.11.5)