##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062998_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	902360
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209788776098232	32.0	32.0	32.0	32.0	32.0
2	30.80853428786737	32.0	32.0	32.0	32.0	32.0
3	30.863941220798793	32.0	32.0	32.0	32.0	32.0
4	30.92913692982845	32.0	32.0	32.0	32.0	32.0
5	30.85643091449089	32.0	32.0	32.0	32.0	32.0
6	34.500386763597675	36.0	36.0	36.0	32.0	36.0
7	34.411302584334415	36.0	36.0	36.0	32.0	36.0
8	34.40110155592003	36.0	36.0	36.0	32.0	36.0
9	34.53783855667361	36.0	36.0	36.0	32.0	36.0
10	34.250193935901414	36.0	36.0	36.0	32.0	36.0
11	34.532566824770605	36.0	36.0	36.0	32.0	36.0
12	34.340941531096234	36.0	36.0	36.0	32.0	36.0
13	34.42991599804956	36.0	36.0	36.0	32.0	36.0
14	34.32842102930094	36.0	36.0	36.0	32.0	36.0
15	34.27213196506937	36.0	36.0	36.0	32.0	36.0
16	34.296860454807394	36.0	36.0	36.0	32.0	36.0
17	34.205550999601044	36.0	36.0	36.0	32.0	36.0
18	34.237775388980005	36.0	36.0	36.0	32.0	36.0
19	34.23050999601046	36.0	36.0	36.0	32.0	36.0
20	34.198022962010725	36.0	36.0	36.0	32.0	36.0
21	34.18712708896671	36.0	36.0	36.0	32.0	36.0
22	34.15739837758766	36.0	36.0	36.0	32.0	36.0
23	34.10905625249346	36.0	36.0	36.0	32.0	36.0
24	34.090417350059845	36.0	36.0	36.0	32.0	36.0
25	34.06892814397801	36.0	36.0	36.0	32.0	36.0
26	34.02001861784654	36.0	36.0	36.0	32.0	36.0
27	34.03208032270934	36.0	36.0	36.0	32.0	36.0
28	34.01227558845694	36.0	36.0	36.0	32.0	36.0
29	33.965645640320936	36.0	36.0	36.0	32.0	36.0
30	33.941060109047385	36.0	36.0	36.0	32.0	36.0
31	33.9615264417749	36.0	36.0	36.0	32.0	36.0
32	33.904378518551354	36.0	36.0	36.0	32.0	36.0
33	33.871674276342034	36.0	36.0	36.0	32.0	36.0
34	33.885494702779376	36.0	36.0	36.0	32.0	36.0
35	33.83483532071457	36.0	36.0	36.0	32.0	36.0
36	33.788893567977304	36.0	36.0	36.0	32.0	36.0
37	33.78295580477858	36.0	36.0	36.0	32.0	36.0
38	33.19153552905714	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	11.0
11	13.0
12	4.0
13	5.0
14	175.0
15	312.0
16	364.0
17	384.0
18	537.0
19	672.0
20	884.0
21	1235.0
22	1908.0
23	2876.0
24	4279.0
25	6341.0
26	9159.0
27	13158.0
28	18306.0
29	24359.0
30	32698.0
31	43851.0
32	59425.0
33	87954.0
34	193508.0
35	399940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57268348539873	17.45473266781839	11.331839863657093	25.64074398312579
2	16.716747572277278	20.0967097399415	37.39979198868134	25.786750699099876
3	18.73476702906221	24.395918145675694	28.647350973192186	28.22196385206991
4	12.392499335047432	15.517665573189113	36.493040163135035	35.59679492862842
5	14.509983842333089	36.70361430829006	33.67000680439471	15.116395044982148
6	33.6068017274721	35.97692271036251	17.107825156063164	13.308450406102226
7	29.938051332062592	30.79325324704109	21.13768340795248	18.131012012943838
8	28.077516819802316	32.61916820245601	19.85229825379921	19.451016723942466
9	27.778442702342087	14.069692960000355	18.82589836849676	39.3259659691608
10	15.981794365429241	26.443052535689826	31.510885923325333	26.0642671755556
11	37.65224271950103	21.17135188210235	22.191918363547302	18.984487034849316
12	24.83922197065157	24.099914554636822	28.723273388590222	22.337590086121388
13	29.518591752860697	19.354863737325818	25.191082520161203	25.935461989652282
14	23.725576905386518	20.16301089140624	25.208058332806882	30.90335387040036
15	25.26032833405385	27.208827995965105	22.61071020806314	24.920133461917906
16	25.667432148462087	25.72373496005604	24.072666093294202	24.53616679818767
17	23.8105108673483	25.865818620365527	25.462782324929723	24.86088818735645
18	25.028593213877237	24.47368537712011	26.633802941776235	23.863918467226412
19	25.47323926179108	25.00410066807641	25.705869053477144	23.816791016655362
20	25.704802119005667	24.090611873967926	25.157429264886787	25.04715674213962
21	27.314136508619065	24.220715458904003	24.333427167400714	24.13172086507622
22	25.741938915967893	24.534688096393875	25.072537011053996	24.650835976584233
23	24.118478290986673	24.217561164833338	25.675653209941533	25.988307334238453
24	24.622435520876053	25.316604413508752	25.275594914822165	24.785365150793034
25	24.930918941566237	24.519480692218252	25.28760220837707	25.261998157838445
26	24.403274756310868	25.595284279972603	25.938122801144793	24.063318162571743
27	25.405885267104587	24.864926569927306	24.85849846002436	24.87068970294374
28	24.291315806389406	24.794138573831937	25.852870312288733	25.06167530748992
29	24.20883813670062	25.216098881794274	25.881657945866387	24.693405035638722
30	24.278865335000134	25.261324041811843	26.15312835243947	24.306682270748553
31	25.039288223728978	25.079850959652266	24.58511857396493	25.295742242653823
32	24.36559137401543	25.1858312728514	24.805025396294127	25.643551956839044
33	24.064680228530896	24.65096948303474	25.48895304698626	25.795397241448104
34	24.97614294945187	24.988113037531768	25.6681913751082	24.36755263790816
35	25.889247016262036	24.618054939793222	25.586107219493165	23.906590824451577
36	24.352093849131137	25.58594745814186	25.071435380076124	24.99052331265088
37	25.32861196081039	25.377598971494436	24.983264618521968	24.310524449173208
38	24.373828005514746	25.193725424134556	25.525541167760895	24.906905402589803
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	463.0
1	357.0
2	251.0
3	251.0
4	501.5
5	752.0
6	752.0
7	887.0
8	1022.0
9	986.5
10	951.0
11	951.0
12	1177.5
13	1404.0
14	1784.0
15	2164.0
16	2164.0
17	3191.5
18	4219.0
19	4219.0
20	5140.0
21	6061.0
22	6583.0
23	7105.0
24	7105.0
25	8312.5
26	9520.0
27	9520.0
28	12213.0
29	14906.0
30	18207.5
31	21509.0
32	21509.0
33	27065.5
34	32622.0
35	32622.0
36	36018.0
37	39414.0
38	45206.5
39	50999.0
40	50999.0
41	54077.0
42	57155.0
43	64310.0
44	71465.0
45	71465.0
46	73514.5
47	75564.0
48	75564.0
49	79475.0
50	83386.0
51	83336.5
52	83287.0
53	83287.0
54	78982.5
55	74678.0
56	74678.0
57	72369.0
58	70060.0
59	62426.0
60	54792.0
61	54792.0
62	52374.0
63	49956.0
64	40965.0
65	31974.0
66	31974.0
67	26792.0
68	21610.0
69	21610.0
70	17322.5
71	13035.0
72	10236.0
73	7437.0
74	7437.0
75	5599.5
76	3762.0
77	3762.0
78	3839.5
79	3917.0
80	3013.5
81	2110.0
82	2110.0
83	2242.5
84	2375.0
85	2375.0
86	1547.5
87	720.0
88	667.0
89	614.0
90	614.0
91	444.5
92	275.0
93	242.5
94	210.0
95	210.0
96	183.5
97	157.0
98	157.0
99	308.0
100	459.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12223502814841082
2	0.05385877033556452
3	0.01363092335653176
4	0.004432820603750166
5	2.216410301875083E-4
6	1.1082051509375415E-4
7	0.0
8	1.1082051509375415E-4
9	2.216410301875083E-4
10	2.216410301875083E-4
11	0.0019947692716875748
12	0.0032137949377188707
13	0.014628307992375548
14	0.00986302584334412
15	0.02548871847156346
16	0.010638769449000399
17	0.023050667139500863
18	0.005319384724500199
19	0.007535795026375282
20	0.005541025754687708
21	0.0064275898754377414
22	0.006870871935812757
23	0.009419743782969104
24	0.01440666696218804
25	0.01762046189990691
26	0.020612615807438273
27	0.008089897601844052
28	0.005984307815062724
29	0.012301077175406712
30	0.0035462564830001327
31	0.005984307815062724
32	0.006981692450906511
33	0.008311538632031561
34	0.012301077175406712
35	0.015071590052750564
36	0.016179795203688105
37	0.008865641207500332
38	0.005762666784875216
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	902360.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.58132836976142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.58225627824916	49.79941857199917
2	11.147093098502689	13.283172285403799
3	2.64171244824068	4.72190210511343
4	0.9338305758400125	2.225554647233887
5	0.4497448183618648	1.3398196852708484
6	0.24508148490852266	0.8761368257810049
7	0.15338763070609435	0.6397327155071656
8	0.11491588309857713	0.5477472772637951
9	0.07517154509263839	0.4030938461004134
>10	0.47604378304226447	5.6290178323236715
>50	0.07749817500201513	3.269343276550294
>100	0.09075305531743154	11.247617723637195
>500	0.010083672782900645	4.131936874398548
>1k	0.002427550855142748	1.8855063334167748
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2522	0.27948933906644796	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1665	0.18451615763110066	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1311	0.1452856952879117	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1290	0.14295846447094288	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1253	0.13885810541247395	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1212	0.13431446429363003	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1149	0.12733277184272354	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1148	0.12722195132762978	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1104	0.12234584866350458	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1095	0.1213484640276608	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1093	0.1211268229974733	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1062	0.11769138702956693	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1043	0.11558579724278559	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	996	0.11037723303337914	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	972	0.10771754067112904	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	971	0.10760672015603528	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	967	0.10716343809566027	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	929	0.1029522585220976	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	919	0.10184405337116008	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	916	0.10151159182587881	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	910	0.10084666873531628	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	905	0.1002925661598475	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.2164103018750832E-4	0.0	0.0	0.0	0.0
7	2.2164103018750832E-4	0.0	0.0	0.0	0.0
8	2.2164103018750832E-4	0.0	0.0	0.0	0.0
9	2.2164103018750832E-4	0.0	0.0	0.0	0.0
10	2.2164103018750832E-4	0.0	0.0	1.1082051509375416E-4	0.0
11	2.2164103018750832E-4	0.0	0.0	1.1082051509375416E-4	0.0
12	2.2164103018750832E-4	0.0	0.0	2.2164103018750832E-4	4.4328206037501665E-4
13	2.2164103018750832E-4	0.0	0.0	2.2164103018750832E-4	5.541025754687707E-4
14	2.2164103018750832E-4	0.0	0.0	2.2164103018750832E-4	5.541025754687707E-4
15	2.2164103018750832E-4	0.0	0.0	2.2164103018750832E-4	5.541025754687707E-4
16	3.3246154528126246E-4	0.0	0.0	2.2164103018750832E-4	5.541025754687707E-4
17	3.3246154528126246E-4	0.0	0.0	2.2164103018750832E-4	5.541025754687707E-4
18	3.3246154528126246E-4	0.0	0.0	3.3246154528126246E-4	6.649230905625249E-4
19	3.3246154528126246E-4	0.0	0.0	3.3246154528126246E-4	6.649230905625249E-4
20	3.3246154528126246E-4	0.0	0.0	5.541025754687707E-4	8.865641207500333E-4
21	3.3246154528126246E-4	0.0	0.0	9.973846358437874E-4	8.865641207500333E-4
22	3.3246154528126246E-4	0.0	0.0	0.0011082051509375415	8.865641207500333E-4
23	3.3246154528126246E-4	0.0	0.0	0.0013298461811250498	8.865641207500333E-4
24	3.3246154528126246E-4	0.0	0.0	0.002216410301875083	8.865641207500333E-4
25	3.3246154528126246E-4	0.0	0.0	0.002216410301875083	8.865641207500333E-4
26	3.3246154528126246E-4	0.0	0.0	0.0028813333924376083	9.973846358437874E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGC	200	0.0	19.197506	30
CTAGCGG	205	0.0	18.730312	29
GTCGTAA	70	1.7885655E-5	18.283339	30
TCTAGCG	215	0.0	17.859137	28
ATTCTAC	90	4.314552E-7	17.797167	1
ACGAACG	135	2.3646862E-11	17.77744	15
CGTCGTA	255	0.0	17.568295	10
GAATTAT	55	0.001354407	17.473581	1
GAGCGTT	55	0.0013655253	17.452278	7
CGAACGA	140	4.0017767E-11	17.14253	16
GATAACG	140	4.0017767E-11	17.14253	11
TACCGTC	290	0.0	17.101227	7
CCGTCGT	265	0.0	16.905338	9
ATACCGT	280	0.0	16.56836	6
ATAACGA	145	6.91216E-11	16.551409	12
ACCGTCG	275	0.0	16.288794	8
GATATAG	60	0.0024236615	16.01745	1
CCTATAC	60	0.0024389613	16.002357	3
CGTGCGC	80	5.619626E-5	15.999695	10
CCGATAA	140	7.421477E-10	15.999695	9
>>END_MODULE