FastQCFastQC Report
Thu 2 Feb 2017
SRR4062998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902360
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30530.33833503258123143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22360.24779467174963432No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13790.152821490314287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13090.14506405425772417No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12800.1418502593200053No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12760.1414069772596303No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12630.1399663105634115No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12510.13863646438228647No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11950.13243051553703622No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11720.12988164368987987No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11310.12533800257103594No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11260.12478389999556719No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10740.11902123321069197No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10630.11780220754466066No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10350.11469923312203555No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10330.11447759209184803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.10572277139944146No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9480.10505784830887893No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT9090.10073584822022252No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9050.1002925661598475No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC9040.10018174564475377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1300.020.9236564
CTAGCGG2350.019.06330329
TCTAGCG2450.018.93825128
TAGCGGC2450.018.28520830
AGGACGT1453.6379788E-1217.656645
TATACTC753.2301432E-517.0680855
GATATAC1151.8373612E-816.6970391
CGAAAAC2400.016.66620423
CGCGGTC1551.0913936E-1116.51658810
CGAGCCG3200.016.49954415
GTAACTG600.00244000616.001335
TATAGGT908.5744505E-616.0004444
TTAGTAG703.694519E-416.0004433
TACCGTC2250.015.6457457
AGCGGCG2900.015.44784731
CAAGACG3350.015.2840054
GTAAACG2100.015.23767327
TCCAACG951.4320889E-515.157474528
ACCGCAG3600.015.11069329
GACGTGA756.238576E-414.9345747
Produced by FastQC (version 0.11.5)