##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062998_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	902360
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21481005363713	32.0	32.0	32.0	32.0	32.0
2	31.329868345228068	32.0	32.0	32.0	32.0	32.0
3	31.39417305731637	32.0	32.0	32.0	32.0	32.0
4	31.489654904915998	32.0	32.0	32.0	32.0	32.0
5	31.42365131433131	32.0	32.0	32.0	32.0	32.0
6	34.942618245489605	36.0	36.0	36.0	36.0	36.0
7	34.9643002792677	36.0	36.0	36.0	36.0	36.0
8	34.89956225896538	36.0	36.0	36.0	36.0	36.0
9	34.99614344607474	36.0	36.0	36.0	36.0	36.0
10	34.859968305332686	36.0	36.0	36.0	32.0	36.0
11	35.024857041535526	36.0	36.0	36.0	36.0	36.0
12	34.921554590185735	36.0	36.0	36.0	32.0	36.0
13	34.97986280420231	36.0	36.0	36.0	36.0	36.0
14	34.91581076288843	36.0	36.0	36.0	32.0	36.0
15	34.89446119065561	36.0	36.0	36.0	32.0	36.0
16	34.90699609911787	36.0	36.0	36.0	32.0	36.0
17	34.86735449266368	36.0	36.0	36.0	32.0	36.0
18	34.86641916751629	36.0	36.0	36.0	32.0	36.0
19	34.86862006294605	36.0	36.0	36.0	32.0	36.0
20	34.84702668558003	36.0	36.0	36.0	32.0	36.0
21	34.845064054257726	36.0	36.0	36.0	32.0	36.0
22	34.815664479808504	36.0	36.0	36.0	32.0	36.0
23	34.76919522141939	36.0	36.0	36.0	32.0	36.0
24	34.73988873620284	36.0	36.0	36.0	32.0	36.0
25	34.72224278558447	36.0	36.0	36.0	32.0	36.0
26	34.64940711024425	36.0	36.0	36.0	32.0	36.0
27	34.636857795115034	36.0	36.0	36.0	32.0	36.0
28	34.60660490269959	36.0	36.0	36.0	32.0	36.0
29	34.56380380336008	36.0	36.0	36.0	32.0	36.0
30	34.54394809167073	36.0	36.0	36.0	32.0	36.0
31	34.53668048229088	36.0	36.0	36.0	32.0	36.0
32	34.513374927966666	36.0	36.0	36.0	32.0	36.0
33	34.474560042555076	36.0	36.0	36.0	32.0	36.0
34	34.45641650782393	36.0	36.0	36.0	32.0	36.0
35	34.41655769316016	36.0	36.0	36.0	32.0	36.0
36	34.395019726051686	36.0	36.0	36.0	32.0	36.0
37	34.37786027749457	36.0	36.0	36.0	32.0	36.0
38	33.95569617447582	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	18.0
22	50.0
23	192.0
24	550.0
25	1472.0
26	3276.0
27	6057.0
28	10762.0
29	16663.0
30	25209.0
31	36209.0
32	51728.0
33	81977.0
34	199215.0
35	468978.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8785652061153	18.612886279151734	12.067115760167225	26.441432754565746
2	15.606188217562837	21.162063921273106	36.70231393235516	26.5294339288089
3	17.726295491821446	25.040781949554503	29.058136442218185	28.17478611640587
4	11.892827577494101	16.350809378528943	36.49578493703726	35.2605781069397
5	13.871514694800302	37.33720466332727	33.3267210425994	15.464559599273016
6	33.53118489294738	36.42548428565096	16.77534465180194	13.267986169599716
7	29.59330907092101	30.932691753587278	21.221890251740998	18.252108923750715
8	27.905108202847508	32.85627368166741	19.71179154480663	19.526826570678452
9	27.611035503876657	13.898887760937312	18.695280187604453	39.794796547581576
10	15.743342982148953	26.742549503743536	31.53389571300019	25.980211801107323
11	37.60050447567002	21.13555788780736	22.153264407534774	19.110673228987846
12	24.575528310857013	24.287615655444576	28.672133091448288	22.46472294225013
13	29.440389024767306	19.272927958827918	25.33370864589371	25.952974370511072
14	23.548029611241635	20.085996719712753	25.278824415975887	31.08714925306973
15	25.147834567135067	27.253645994946584	22.52338312868478	25.075136309233564
16	25.74471386143003	25.727869143135777	23.92249213174343	24.604924863690766
17	23.8874728489738	25.918369608581944	25.32237687840773	24.871780664036528
18	24.961545281262467	24.405891218582383	26.643579059355467	23.988984440799683
19	25.361496520235825	25.05308302672991	25.702823706724587	23.882596746309677
20	25.570614832217743	24.00161797952037	25.481293497052178	24.946473691209718
21	27.128892159329048	24.247555573779394	24.462658431954466	24.16089383493709
22	25.627022474400462	24.35380557648832	25.240480517753443	24.77869143135777
23	23.937811890832805	23.984799841304845	25.887590194146675	26.18979807371567
24	24.57721973491733	25.18562436278204	25.516423600336896	24.72073230196374
25	24.829225586240526	24.386165166895697	25.508887805310522	25.275721441553262
26	24.487565938206483	25.45336672724855	25.94806950662707	24.110997827917906
27	25.37149848341957	24.730844375686395	25.058319360697904	24.83933778019613
28	24.201538188749502	24.610909171505828	26.171040382995702	25.016512256748968
29	24.134602597632874	24.87887317700253	26.15929340839576	24.827230816968836
30	24.2157232146815	24.89582871581187	26.401657874905805	24.486790194600825
31	24.90258876723259	24.897380203023182	24.862693381798838	25.337337647945386
32	24.408329269914447	24.943813998847467	25.018174564475377	25.629682166762713
33	24.11410080234053	24.345715678886474	25.83868965822953	25.70149386054346
34	24.916330511104213	24.779134713418145	25.930892326787536	24.373642448690102
35	25.839797863380472	24.409991577640852	25.701161398998185	24.049049159980495
36	24.345383217341194	25.24036969723835	25.409481803271422	25.004765282149034
37	25.470100625027705	25.038787180282814	25.142182720865286	24.348929473824192
38	24.33125211944705	24.811660117159708	25.75762613064881	25.09946163274443
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	210.0
1	165.0
2	120.0
3	120.0
4	420.0
5	720.0
6	720.0
7	884.5
8	1049.0
9	1005.0
10	961.0
11	961.0
12	1250.0
13	1539.0
14	1933.5
15	2328.0
16	2328.0
17	3565.5
18	4803.0
19	4803.0
20	5598.0
21	6393.0
22	6757.0
23	7121.0
24	7121.0
25	8287.5
26	9454.0
27	9454.0
28	12052.0
29	14650.0
30	18006.0
31	21362.0
32	21362.0
33	27044.5
34	32727.0
35	32727.0
36	36240.5
37	39754.0
38	45408.5
39	51063.0
40	51063.0
41	53911.5
42	56760.0
43	64310.5
44	71861.0
45	71861.0
46	73861.0
47	75861.0
48	75861.0
49	79758.0
50	83655.0
51	83836.5
52	84018.0
53	84018.0
54	79487.0
55	74956.0
56	74956.0
57	72612.5
58	70269.0
59	62549.0
60	54829.0
61	54829.0
62	52279.0
63	49729.0
64	40770.0
65	31811.0
66	31811.0
67	26728.5
68	21646.0
69	21646.0
70	17424.5
71	13203.0
72	10280.5
73	7358.0
74	7358.0
75	5440.5
76	3523.0
77	3523.0
78	3652.5
79	3782.0
80	2866.0
81	1950.0
82	1950.0
83	1959.5
84	1969.0
85	1969.0
86	1236.0
87	503.0
88	420.0
89	337.0
90	337.0
91	196.5
92	56.0
93	33.0
94	10.0
95	10.0
96	9.5
97	9.0
98	9.0
99	10.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010860410479187907
2	0.0
3	0.0
4	1.1082051509375415E-4
5	0.0
6	0.0
7	4.432820603750166E-4
8	0.002992153907531362
9	0.0039895385433751495
10	4.432820603750166E-4
11	0.004100359058468904
12	3.3246154528126246E-4
13	1.1082051509375415E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1082051509375415E-4
22	0.0
23	1.1082051509375415E-4
24	0.0
25	0.0
26	0.0
27	1.1082051509375415E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.216410301875083E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	902360.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.604436254361715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.42523593689968	47.48059249277862
2	11.860465334707513	13.664308386404514
3	2.8637700192795794	4.948975725682282
4	1.0359531125298294	2.387019801329286
5	0.48338267515021943	1.3922493248576824
6	0.2698609729140166	0.9327113527059301
7	0.1690593444254149	0.6816997760410596
8	0.12051800499411448	0.5553897388949052
9	0.08298989836164679	0.4302527678936495
>10	0.49409192333281476	5.6680664951324795
>50	0.08310074683787291	3.3843302984360037
>100	0.09762552676902984	11.777669560739703
>500	0.010847280732013596	4.255796559962993
>1k	0.0030992230662895987	2.4409377191408312
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3053	0.33833503258123143	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2236	0.24779467174963432	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1379	0.152821490314287	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1309	0.14506405425772417	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1280	0.1418502593200053	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1276	0.1414069772596303	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1263	0.1399663105634115	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1251	0.13863646438228647	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1195	0.13243051553703622	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1172	0.12988164368987987	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1131	0.12533800257103594	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1126	0.12478389999556719	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1074	0.11902123321069197	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1063	0.11780220754466066	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1035	0.11469923312203555	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1033	0.11447759209184803	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	954	0.10572277139944146	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	948	0.10505784830887893	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	909	0.10073584822022252	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	905	0.1002925661598475	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	904	0.10018174564475377	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1082051509375416E-4
2	0.0	0.0	0.0	0.0	1.1082051509375416E-4
3	0.0	0.0	0.0	0.0	1.1082051509375416E-4
4	0.0	0.0	0.0	0.0	1.1082051509375416E-4
5	0.0	0.0	0.0	0.0	1.1082051509375416E-4
6	0.0	0.0	0.0	0.0	1.1082051509375416E-4
7	0.0	0.0	0.0	0.0	1.1082051509375416E-4
8	0.0	0.0	0.0	0.0	1.1082051509375416E-4
9	0.0	0.0	0.0	0.0	1.1082051509375416E-4
10	0.0	0.0	0.0	1.1082051509375416E-4	1.1082051509375416E-4
11	0.0	0.0	0.0	1.1082051509375416E-4	1.1082051509375416E-4
12	0.0	0.0	0.0	1.1082051509375416E-4	2.2164103018750832E-4
13	0.0	0.0	0.0	1.1082051509375416E-4	3.3246154528126246E-4
14	0.0	0.0	0.0	1.1082051509375416E-4	3.3246154528126246E-4
15	0.0	0.0	0.0	1.1082051509375416E-4	6.649230905625249E-4
16	0.0	0.0	0.0	1.1082051509375416E-4	6.649230905625249E-4
17	0.0	0.0	0.0	1.1082051509375416E-4	6.649230905625249E-4
18	0.0	0.0	0.0	1.1082051509375416E-4	6.649230905625249E-4
19	0.0	0.0	0.0	1.1082051509375416E-4	6.649230905625249E-4
20	0.0	0.0	0.0	3.3246154528126246E-4	7.757436056562791E-4
21	0.0	0.0	0.0	7.757436056562791E-4	7.757436056562791E-4
22	0.0	0.0	0.0	8.865641207500333E-4	8.865641207500333E-4
23	0.0	0.0	0.0	0.0012190256660312958	8.865641207500333E-4
24	0.0	0.0	0.0	0.002105589786781329	8.865641207500333E-4
25	0.0	0.0	0.0	0.002216410301875083	8.865641207500333E-4
26	0.0	0.0	0.0	0.0028813333924376083	9.973846358437874E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	130	0.0	20.923656	4
CTAGCGG	235	0.0	19.063303	29
TCTAGCG	245	0.0	18.938251	28
TAGCGGC	245	0.0	18.285208	30
AGGACGT	145	3.6379788E-12	17.65664	5
TATACTC	75	3.2301432E-5	17.068085	5
GATATAC	115	1.8373612E-8	16.697039	1
CGAAAAC	240	0.0	16.666204	23
CGCGGTC	155	1.0913936E-11	16.516588	10
CGAGCCG	320	0.0	16.499544	15
GTAACTG	60	0.002440006	16.00133	5
TATAGGT	90	8.5744505E-6	16.000444	4
TTAGTAG	70	3.694519E-4	16.000443	3
TACCGTC	225	0.0	15.645745	7
AGCGGCG	290	0.0	15.447847	31
CAAGACG	335	0.0	15.284005	4
GTAAACG	210	0.0	15.237673	27
TCCAACG	95	1.4320889E-5	15.1574745	28
ACCGCAG	360	0.0	15.110693	29
GACGTGA	75	6.238576E-4	14.934574	7
>>END_MODULE