FastQCFastQC Report
Thu 2 Feb 2017
SRR4062997_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062997_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466275
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23750.1619750728887828No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23520.16040647218291249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20560.1402192630986684No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20370.13892346251555815No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT20130.1372866617789978No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19030.12978465840309628No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18960.1293072581882662No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18470.1259654566844555No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18060.12316925542616493No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18060.12316925542616493No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC17400.11866805340062403No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16560.11293925082266286No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16550.11287105079197286No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16340.11143885014748256No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15970.10891544901195206No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15540.10598284769228147No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14960.10202724591226067No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14940.10189084585088062No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14890.10154984569743057No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14840.10120884554398049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTC2050.020.2889844
CGTCTTA1450.018.75840215
GTATCAA17400.018.590981
ACGCTAC450.00885200117.7775713
ACCGCGT450.00885374117.7769648
CTAGCGG4650.017.204129
TCTAGCG4700.017.02049828
TAGCGGC4750.016.84190830
CAAGACG6400.016.4969944
CGCTTCG3300.016.48465532
GGTATCA7350.016.1229521
CGCGTAA3400.015.99981110
TTATACC600.002445309715.9970844
AAGACGG6650.015.8768065
ATCGTTT3550.015.77446229
TCGCGTA3400.015.5292299
GCGTAAC3550.015.32376311
CGGTCCA5450.015.26587610
AACGCTT3800.015.15771730
AACCGCG2650.015.0936487
Produced by FastQC (version 0.11.5)