FastQCFastQC Report
Thu 2 Feb 2017
SRR4062997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466275
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25150.17152307718538473No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24220.16518047433121344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT21610.14738026632111983No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20550.1401510630679784No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20380.13899166254624815No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20180.13762766193244788No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19850.1353770609196774No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19810.13510426079691734No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19060.12998925849516632No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18160.12385125573306507No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17750.1210550544747745No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17190.11723585275613374No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16810.11464425158991322No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16380.11171165027024262No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15800.10775604849022181No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15720.10721044824470172No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15450.10536904741607134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15140.10325484646468092No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14880.10148164566674055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16850.018.8016661
TCTAGCG4600.018.78244828
GGTATCA7250.018.75911
CTAGCGG4550.018.63720329
ACCCGTT2350.018.38282230
TAACCCG2300.018.08680228
TAGCGGC4750.017.8524830
CCGTTGA2600.017.84600332
ATAGGCG450.00885183917.7776285
ACCGTCG3750.017.493788
GATCTAA550.001364654717.4549921
ATACCGT3950.017.0130926
TACCGTC3950.017.0130927
AACCCGT2450.016.97944829
GCGCAAG5300.016.6041981
AAGACGG5150.016.465885
CAAGACG5150.016.465884
GCGGGTA2550.016.31358723
CGCAAGA5100.016.3135872
CGCGGTC2100.016.00040810
Produced by FastQC (version 0.11.5)