##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062997_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1466275
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21678061755128	32.0	32.0	32.0	32.0	32.0
2	31.21373548618097	32.0	32.0	32.0	32.0	32.0
3	31.32008593203867	32.0	32.0	32.0	32.0	32.0
4	31.43953623979131	32.0	32.0	32.0	32.0	32.0
5	31.3367035515166	32.0	32.0	32.0	32.0	32.0
6	34.87122402005081	36.0	36.0	36.0	32.0	36.0
7	34.9039975447989	36.0	36.0	36.0	36.0	36.0
8	34.82206611992975	36.0	36.0	36.0	32.0	36.0
9	34.96291077730985	36.0	36.0	36.0	32.0	36.0
10	34.78813592266116	36.0	36.0	36.0	32.0	36.0
11	34.98749075037084	36.0	36.0	36.0	36.0	36.0
12	34.86416804487562	36.0	36.0	36.0	32.0	36.0
13	34.933107363898316	36.0	36.0	36.0	32.0	36.0
14	34.873455525054986	36.0	36.0	36.0	32.0	36.0
15	34.84681454706654	36.0	36.0	36.0	32.0	36.0
16	34.85634686535609	36.0	36.0	36.0	32.0	36.0
17	34.81967707285468	36.0	36.0	36.0	32.0	36.0
18	34.819569316806195	36.0	36.0	36.0	32.0	36.0
19	34.81863975038789	36.0	36.0	36.0	32.0	36.0
20	34.809773746398186	36.0	36.0	36.0	32.0	36.0
21	34.80456803805562	36.0	36.0	36.0	32.0	36.0
22	34.788528072837636	36.0	36.0	36.0	32.0	36.0
23	34.72408927384017	36.0	36.0	36.0	32.0	36.0
24	34.6999948849977	36.0	36.0	36.0	32.0	36.0
25	34.68712281120526	36.0	36.0	36.0	32.0	36.0
26	34.61312509590629	36.0	36.0	36.0	32.0	36.0
27	34.60565241854359	36.0	36.0	36.0	32.0	36.0
28	34.57639051337573	36.0	36.0	36.0	32.0	36.0
29	34.54383932072769	36.0	36.0	36.0	32.0	36.0
30	34.520624030280814	36.0	36.0	36.0	32.0	36.0
31	34.52363983563792	36.0	36.0	36.0	32.0	36.0
32	34.50379703670867	36.0	36.0	36.0	32.0	36.0
33	34.46984501543026	36.0	36.0	36.0	32.0	36.0
34	34.46861877887845	36.0	36.0	36.0	32.0	36.0
35	34.43694668462601	36.0	36.0	36.0	32.0	36.0
36	34.402258103016145	36.0	36.0	36.0	32.0	36.0
37	34.40340659153296	36.0	36.0	36.0	32.0	36.0
38	33.95409114934102	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	20.0
22	85.0
23	330.0
24	862.0
25	2149.0
26	4809.0
27	9460.0
28	16533.0
29	26890.0
30	41155.0
31	60798.0
32	89392.0
33	145301.0
34	350042.0
35	718445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96915657161253	18.301507284058147	11.886393383473827	26.842942760855493
2	16.352662358698062	19.981381391621625	35.905406557432954	27.76054969224736
3	18.059027126562206	23.488840769978346	28.514160031372015	29.937972072087433
4	12.232118073671238	15.556333038026397	35.557206000800676	36.6543428875017
5	14.601080970486437	36.4842202178991	33.01120185504083	15.903496956573631
6	35.65029455613344	35.322661385252694	15.743101221190583	13.283942837423293
7	31.049371601917247	30.06005723373899	20.2033325422092	18.68723862213456
8	28.757550770985123	32.78758403291137	18.540676464510266	19.91418873159324
9	27.20392354248254	13.809399096728608	18.006296437247737	40.98038092354112
10	15.894943956242564	26.20563131493966	30.940859940051762	26.958564788766015
11	38.81045836331457	20.7402835229966	21.243013378165934	19.2062447355229
12	24.69804333444727	23.501333315146596	27.909661931295055	23.89096141911108
13	29.466818252808313	18.563528074239926	25.113536155954584	26.856117516997173
14	23.546417654544786	19.340109693004173	24.232169430815794	32.88130322163525
15	25.53484169067876	26.921280114576053	21.393053826874223	26.150824367870968
16	26.596238768307444	25.451228453052803	22.635249185862133	25.317283592777617
17	24.781026751462036	25.83608122623655	24.14506146527766	25.23783055702375
18	25.986939694122864	24.281597926719066	25.11268350070758	24.618778878450495
19	25.97630048933522	24.68902491006121	24.658880496496224	24.675794104107347
20	26.186629383983224	23.824384920973216	24.028780412951185	25.960205282092375
21	27.559837001926653	23.778486300318836	23.725426676442005	24.93625002131251
22	26.35290105880548	23.734019880308946	24.52882303797037	25.38425602291521
23	24.808766722681742	23.350306082363982	25.216467340302483	26.6244598546518
24	25.223167550425394	24.623825680721556	24.531210039044517	25.621796729808526
25	25.278358615101954	24.087858067455333	24.66933192568374	25.964451391758974
26	25.184429932993467	25.116980102641044	24.918790813455864	24.779799150909618
27	26.09730514214942	24.07810545641538	24.159809148903957	25.664780252531244
28	25.04816627167482	24.264957119230704	25.198001739100782	25.488874869993694
29	25.00090365040664	24.382806772263045	25.184634533085543	25.43165504424477
30	24.924656016095206	24.609367274215273	25.318852193483487	25.14712451620603
31	25.611907725358474	24.480128216057697	24.076315834342125	25.8316482242417
32	25.463572658607696	24.30287633629435	23.9590117815553	26.274539223542654
33	24.946684626008082	23.867623740430684	24.777071149682016	26.40862048387922
34	25.940495473222963	24.074542633544187	24.80196416088387	25.182997732348976
35	26.394093877342247	23.89769995396498	24.924860616187278	24.783345552505498
36	25.011270055071527	24.698913914511262	24.40538098242144	25.884435047995773
37	26.26690082010537	24.42625019181259	24.280643126289405	25.02620586179264
38	25.1330071098532	23.867350940307926	25.024841861178835	25.97480008866004
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	26.5
2	44.0
3	44.0
4	147.5
5	251.0
6	251.0
7	348.5
8	446.0
9	473.0
10	500.0
11	500.0
12	697.0
13	894.0
14	1386.0
15	1878.0
16	1878.0
17	2903.5
18	3929.0
19	3929.0
20	4942.5
21	5956.0
22	7104.5
23	8253.0
24	8253.0
25	9941.5
26	11630.0
27	11630.0
28	15047.0
29	18464.0
30	22914.5
31	27365.0
32	27365.0
33	36202.5
34	45040.0
35	45040.0
36	51114.5
37	57189.0
38	66347.5
39	75506.0
40	75506.0
41	81430.5
42	87355.0
43	101398.5
44	115442.0
45	115442.0
46	116964.5
47	118487.0
48	118487.0
49	127752.5
50	137018.0
51	140453.0
52	143888.0
53	143888.0
54	138135.0
55	132382.0
56	132382.0
57	128366.5
58	124351.0
59	111296.5
60	98242.0
61	98242.0
62	93683.0
63	89124.0
64	74451.5
65	59779.0
66	59779.0
67	50586.0
68	41393.0
69	41393.0
70	33283.0
71	25173.0
72	19551.0
73	13929.0
74	13929.0
75	10576.5
76	7224.0
77	7224.0
78	6845.5
79	6467.0
80	5101.5
81	3736.0
82	3736.0
83	3488.5
84	3241.0
85	3241.0
86	2078.5
87	916.0
88	753.0
89	590.0
90	590.0
91	349.0
92	108.0
93	75.5
94	43.0
95	43.0
96	29.5
97	16.0
98	16.0
99	16.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008729603928321769
2	0.0
3	0.0
4	2.728001227600553E-4
5	0.0
6	6.820003069001382E-5
7	4.774002148300967E-4
8	0.0023188010434604697
9	0.0033418015038106765
10	6.820003069001381E-4
11	0.005456002455201105
12	3.4100015345006905E-4
13	1.3640006138002764E-4
14	6.820003069001382E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.0460009207004144E-4
24	0.0
25	6.820003069001382E-5
26	0.0
27	6.820003069001382E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1466275.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.66862043433133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.85228367232594	45.566037114379284
2	11.396399135294633	12.688436355617174
3	3.055475348150366	5.102822922079174
4	1.2359165063445634	2.7520706752088144
5	0.6870270714389901	1.9122924634023688
6	0.3776223556338898	1.2613029349980656
7	0.2541631146119994	0.9904236969019993
8	0.17234741713426605	0.7675474357827864
9	0.13783737747317557	0.6905894983396076
>10	0.6687756175701723	6.731298569628164
>50	0.06581996489430268	2.612536877849355
>100	0.07824708924889251	9.517225312559493
>500	0.01254900440576278	4.7914764692154375
>1k	0.005536325473130639	4.615939674038206
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2515	0.17152307718538473	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2422	0.16518047433121344	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2161	0.14738026632111983	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2055	0.1401510630679784	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2038	0.13899166254624815	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2018	0.13762766193244788	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1985	0.1353770609196774	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1981	0.13510426079691734	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1906	0.12998925849516632	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1816	0.12385125573306507	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1775	0.1210550544747745	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1719	0.11723585275613374	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1681	0.11464425158991322	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1638	0.11171165027024262	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1580	0.10775604849022181	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1572	0.10721044824470172	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1545	0.10536904741607134	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1514	0.10325484646468092	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1488	0.10148164566674055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	6.820003069001381E-5	0.0	0.0	0.0
6	0.0	6.820003069001381E-5	0.0	0.0	6.820003069001381E-5
7	0.0	6.820003069001381E-5	0.0	0.0	6.820003069001381E-5
8	0.0	6.820003069001381E-5	0.0	0.0	1.3640006138002762E-4
9	0.0	6.820003069001381E-5	0.0	0.0	2.0460009207004144E-4
10	0.0	6.820003069001381E-5	0.0	0.0	2.0460009207004144E-4
11	0.0	6.820003069001381E-5	0.0	0.0	2.0460009207004144E-4
12	0.0	6.820003069001381E-5	0.0	0.0	4.774002148300967E-4
13	0.0	6.820003069001381E-5	0.0	0.0	4.774002148300967E-4
14	0.0	6.820003069001381E-5	0.0	0.0	4.774002148300967E-4
15	0.0	6.820003069001381E-5	0.0	6.820003069001381E-5	6.138002762101243E-4
16	0.0	6.820003069001381E-5	0.0	6.820003069001381E-5	6.138002762101243E-4
17	0.0	6.820003069001381E-5	0.0	6.820003069001381E-5	6.138002762101243E-4
18	0.0	6.820003069001381E-5	0.0	6.820003069001381E-5	6.820003069001381E-4
19	0.0	6.820003069001381E-5	0.0	6.820003069001381E-5	6.820003069001381E-4
20	0.0	6.820003069001381E-5	0.0	6.820003069001381E-5	6.820003069001381E-4
21	0.0	6.820003069001381E-5	0.0	1.3640006138002762E-4	7.502003375901519E-4
22	0.0	6.820003069001381E-5	0.0	2.0460009207004144E-4	8.866003989701796E-4
23	0.0	6.820003069001381E-5	0.0	2.7280012276005523E-4	9.548004296601934E-4
24	0.0	6.820003069001381E-5	0.0	3.4100015345006905E-4	9.548004296601934E-4
25	0.0	6.820003069001381E-5	0.0	4.774002148300967E-4	9.548004296601934E-4
26	0.0	6.820003069001381E-5	0.0	6.138002762101243E-4	9.548004296601934E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1685	0.0	18.801666	1
TCTAGCG	460	0.0	18.782448	28
GGTATCA	725	0.0	18.7591	1
CTAGCGG	455	0.0	18.637203	29
ACCCGTT	235	0.0	18.382822	30
TAACCCG	230	0.0	18.086802	28
TAGCGGC	475	0.0	17.85248	30
CCGTTGA	260	0.0	17.846003	32
ATAGGCG	45	0.008851839	17.777628	5
ACCGTCG	375	0.0	17.49378	8
GATCTAA	55	0.0013646547	17.454992	1
ATACCGT	395	0.0	17.013092	6
TACCGTC	395	0.0	17.013092	7
AACCCGT	245	0.0	16.979448	29
GCGCAAG	530	0.0	16.604198	1
AAGACGG	515	0.0	16.46588	5
CAAGACG	515	0.0	16.46588	4
GCGGGTA	255	0.0	16.313587	23
CGCAAGA	510	0.0	16.313587	2
CGCGGTC	210	0.0	16.000408	10
>>END_MODULE