FastQCFastQC Report
Thu 2 Feb 2017
SRR4062996_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062996_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835256
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA25830.3092465064602948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24630.29487965366306856No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC20280.24279981227312347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15940.19083969465648853No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG11550.13828095817330255No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10120.12116045858994128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9650.11553344124436102No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9560.11445592728456903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT1400.019.42602729
CTAGCGG2200.018.17943629
GTAAACG1251.4733814E-1017.91765227
GTATTAG1700.017.8971561
GTCGTAC450.00880622917.7924941
CGCCGGT2400.017.3310627
CGGTCCA2600.017.2326410
TCTAGCG2350.017.01904928
CGCTTCG1252.8539944E-916.63782132
CGCGGTT2450.016.32830210
GCCGGTC2550.016.3115888
CCGGTCC3100.016.0017389
CGAACGA1001.3118297E-616.00173616
CATCGTT1703.6379788E-1215.99790628
TAGCGGC2500.015.99790530
AAACGCT1304.802132E-915.99790529
TAGGACA1501.1459633E-1015.9959894
AGGACCG3550.015.7716385
ATACCGT1652.910383E-1115.5121916
CTTGCGC2900.015.4462533
Produced by FastQC (version 0.11.5)