##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062996_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835256
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.256345359985442	32.0	32.0	32.0	32.0	32.0
2	30.91698952177536	32.0	32.0	32.0	32.0	32.0
3	30.973287231699025	32.0	32.0	32.0	32.0	32.0
4	31.028599614968346	32.0	32.0	32.0	32.0	32.0
5	30.962118200886913	32.0	32.0	32.0	32.0	32.0
6	34.600661354123766	36.0	36.0	36.0	32.0	36.0
7	34.5317806756252	36.0	36.0	36.0	32.0	36.0
8	34.523571216489316	36.0	36.0	36.0	32.0	36.0
9	34.639996599844835	36.0	36.0	36.0	32.0	36.0
10	34.40611620868333	36.0	36.0	36.0	32.0	36.0
11	34.64306631739251	36.0	36.0	36.0	32.0	36.0
12	34.47893460208607	36.0	36.0	36.0	32.0	36.0
13	34.5538972482688	36.0	36.0	36.0	32.0	36.0
14	34.45878149932476	36.0	36.0	36.0	32.0	36.0
15	34.401144080377755	36.0	36.0	36.0	32.0	36.0
16	34.417454050015806	36.0	36.0	36.0	32.0	36.0
17	34.35898694531976	36.0	36.0	36.0	32.0	36.0
18	34.3644678996619	36.0	36.0	36.0	32.0	36.0
19	34.35995072169491	36.0	36.0	36.0	32.0	36.0
20	34.327547482448495	36.0	36.0	36.0	32.0	36.0
21	34.29189613723218	36.0	36.0	36.0	32.0	36.0
22	34.27552151675654	36.0	36.0	36.0	32.0	36.0
23	34.2168664457364	36.0	36.0	36.0	32.0	36.0
24	34.21296464796422	36.0	36.0	36.0	32.0	36.0
25	34.166517810108516	36.0	36.0	36.0	32.0	36.0
26	34.12965246583084	36.0	36.0	36.0	32.0	36.0
27	34.10867686074688	36.0	36.0	36.0	32.0	36.0
28	34.08792394188129	36.0	36.0	36.0	32.0	36.0
29	34.051991245797694	36.0	36.0	36.0	32.0	36.0
30	34.01024715775762	36.0	36.0	36.0	32.0	36.0
31	34.02361072533451	36.0	36.0	36.0	32.0	36.0
32	33.96628219372264	36.0	36.0	36.0	32.0	36.0
33	33.93972147461377	36.0	36.0	36.0	32.0	36.0
34	33.92537377762027	36.0	36.0	36.0	32.0	36.0
35	33.87970274981563	36.0	36.0	36.0	32.0	36.0
36	33.825922830844675	36.0	36.0	36.0	32.0	36.0
37	33.809651172814085	36.0	36.0	36.0	32.0	36.0
38	33.190312910053926	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	3.0
9	4.0
10	13.0
11	5.0
12	4.0
13	3.0
14	78.0
15	201.0
16	267.0
17	351.0
18	382.0
19	598.0
20	758.0
21	1033.0
22	1389.0
23	2162.0
24	3300.0
25	4979.0
26	7320.0
27	10626.0
28	15569.0
29	21529.0
30	29234.0
31	39679.0
32	54323.0
33	82019.0
34	182008.0
35	377417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.572510080500166	16.569297745132353	11.06661520537371	25.791576968993773
2	16.478714305223136	19.28602060887461	37.828976528116186	26.406288557786066
3	19.922385757870387	23.430592607848112	27.486840781841952	29.160180852439545
4	12.548010612620205	14.979550591931343	35.61333856940347	36.85910022604498
5	14.458339070104339	36.71525462283973	33.641462786813605	15.184943520242323
6	34.88068884517625	34.75449953487147	16.787129169245727	13.577682450706552
7	30.965835624048193	30.051624891051365	20.28515808327028	18.69738140163016
8	28.65251606399498	32.94067785449439	19.023577287360833	19.383228794149794
9	27.59744030266206	14.499524097431324	18.060233102465713	39.8428024974409
10	15.788547394735236	26.700836036113696	31.714059606034596	25.796556963116473
11	37.74120514874292	21.220545644149595	21.572902411662376	19.465346795445104
12	25.12562453081617	23.625673020627925	28.367018786509533	22.881683662046363
13	30.19380332285586	19.449184253854213	24.760604699895225	25.596407723394705
14	23.6762511883865	19.767686902963003	24.7538812643235	31.802180644326995
15	25.314207633913227	27.17125109426289	22.71324504183647	24.801296229987415
16	25.703858123180712	25.63105656848435	24.265548460330937	24.399536848004004
17	24.00184416781927	25.89379269877195	25.50017064539886	24.604192488009915
18	25.06106292594384	24.53616122528149	26.85834837955757	23.5444274692171
19	25.360578362185187	25.395420900254557	25.470733465359814	23.773267272200442
20	25.545559256607415	24.05537369581824	26.039813313729198	24.359253733845147
21	26.30110021946918	24.346112354330774	24.455906810010095	24.896880616189954
22	25.46456828674879	25.03651889882948	25.05483821529148	24.444074599130253
23	23.89070488643031	24.834345103392124	25.630111234838417	25.644838775339153
24	24.47334483654621	25.393681694684293	25.461335913373862	24.671637555395634
25	24.91686674130798	24.674382263308207	25.295382017830093	25.11336897755372
26	23.89169433863302	25.579315060455215	25.94993779046583	24.579052810445933
27	25.200678186903712	25.301615962297408	25.152903367945136	24.34480248285375
28	24.338963893851083	24.788195458834007	25.684383695480385	25.18845695183452
29	24.00238037365161	25.517294228791265	25.93350040650747	24.54682499104965
30	24.454339304571455	25.24789697638723	26.16573238859901	24.1320313304423
31	24.684088400171454	25.2415583296017	25.034662273318308	25.039690996908533
32	24.152711825037056	25.502338378867663	25.140745742905242	25.20420405319004
33	23.76059206611293	25.147124501752323	25.81955681250801	25.272726619626738
34	24.468288921298857	25.538775813222784	25.920391218654732	24.072544046823623
35	25.215959574187828	25.264216690016884	25.76798266096682	23.751841074828466
36	23.972966888749454	25.899866367150263	25.503877268525393	24.623289475574886
37	25.063071067053492	25.62487352859122	25.227828140172825	24.08422726418246
38	24.142749213061798	25.707047063035276	25.277089651687803	24.873114072215124
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	70.0
2	96.0
3	96.0
4	257.5
5	419.0
6	419.0
7	582.0
8	745.0
9	761.5
10	778.0
11	778.0
12	1001.0
13	1224.0
14	1589.0
15	1954.0
16	1954.0
17	2773.0
18	3592.0
19	3592.0
20	4305.5
21	5019.0
22	5293.0
23	5567.0
24	5567.0
25	6634.0
26	7701.0
27	7701.0
28	10182.0
29	12663.0
30	16124.0
31	19585.0
32	19585.0
33	24804.0
34	30023.0
35	30023.0
36	33366.0
37	36709.0
38	41762.0
39	46815.0
40	46815.0
41	50364.5
42	53914.0
43	59705.0
44	65496.0
45	65496.0
46	71895.0
47	78294.0
48	78294.0
49	78566.5
50	78839.0
51	77776.5
52	76714.0
53	76714.0
54	72847.5
55	68981.0
56	68981.0
57	66975.0
58	64969.0
59	58458.0
60	51947.0
61	51947.0
62	48111.0
63	44275.0
64	36783.5
65	29292.0
66	29292.0
67	24430.5
68	19569.0
69	19569.0
70	15525.0
71	11481.0
72	9138.0
73	6795.0
74	6795.0
75	5260.0
76	3725.0
77	3725.0
78	3327.0
79	2929.0
80	2354.5
81	1780.0
82	1780.0
83	1667.0
84	1554.0
85	1554.0
86	1060.0
87	566.0
88	483.5
89	401.0
90	401.0
91	288.0
92	175.0
93	152.0
94	129.0
95	129.0
96	119.0
97	109.0
98	109.0
99	248.5
100	388.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11637150765753254
2	0.05627017345580278
3	0.011972377331021867
4	0.002873370559445248
5	1.1972377331021867E-4
6	3.59171319930656E-4
7	0.0
8	3.59171319930656E-4
9	1.1972377331021867E-4
10	5.986188665510933E-4
11	0.0027536467861350293
12	0.0034719894259963415
13	0.015683814303638643
14	0.01077513959791968
15	0.026219506354937888
16	0.013049891290813836
17	0.022867240702251764
18	0.006225636212131371
19	0.008380664131715307
20	0.006943978851992683
21	0.007063702625302901
22	0.008620111678335743
23	0.010296244504678805
24	0.015204919210397771
25	0.018078289769843017
26	0.020951660329288267
27	0.010535692051299242
28	0.006225636212131371
29	0.011134310917850337
30	0.0035917131993065603
31	0.006465083758751807
32	0.007422873945233556
33	0.009458178091507273
34	0.014606300343846676
35	0.017479670903291923
36	0.015803538076948863
37	0.010415968277989024
38	0.006824255078682464
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	835256.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.54695914651921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.21488121443414	48.567671358332355
2	12.7130511777336	15.394731805716885
3	3.6030240155562594	6.544564436214374
4	1.3266285784693927	3.2129330537276473
5	0.6673840212883633	2.020403653599312
6	0.3267290975080117	1.1869471991278007
7	0.21089360811089672	0.8938276672186748
8	0.1412879668555735	0.6843645405679335
9	0.09983072311872083	0.5440002042812997
>10	0.5491424505302908	6.372331063268384
>50	0.07060007679186107	3.0396422664512612
>100	0.07059781550598582	8.29633933942073
>500	0.004759403277539496	1.9412876897531128
>1k	0.001189850819384874	1.3009557223203227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2583	0.3092465064602948	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2463	0.29487965366306856	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2028	0.24279981227312347	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1594	0.19083969465648853	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1155	0.13828095817330255	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1012	0.12116045858994128	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	965	0.11553344124436102	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	956	0.11445592728456903	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1972377331021867E-4
2	0.0	0.0	0.0	0.0	1.1972377331021867E-4
3	0.0	0.0	0.0	0.0	1.1972377331021867E-4
4	0.0	0.0	0.0	1.1972377331021867E-4	1.1972377331021867E-4
5	0.0	0.0	0.0	1.1972377331021867E-4	1.1972377331021867E-4
6	0.0	0.0	0.0	1.1972377331021867E-4	1.1972377331021867E-4
7	0.0	0.0	0.0	2.3944754662043734E-4	1.1972377331021867E-4
8	0.0	0.0	0.0	2.3944754662043734E-4	1.1972377331021867E-4
9	0.0	0.0	0.0	2.3944754662043734E-4	1.1972377331021867E-4
10	0.0	0.0	0.0	2.3944754662043734E-4	2.3944754662043734E-4
11	0.0	0.0	0.0	3.59171319930656E-4	2.3944754662043734E-4
12	0.0	0.0	0.0	3.59171319930656E-4	3.59171319930656E-4
13	0.0	0.0	0.0	3.59171319930656E-4	3.59171319930656E-4
14	0.0	0.0	0.0	3.59171319930656E-4	3.59171319930656E-4
15	0.0	0.0	0.0	4.7889509324087467E-4	4.7889509324087467E-4
16	0.0	0.0	0.0	4.7889509324087467E-4	5.986188665510933E-4
17	0.0	0.0	0.0	4.7889509324087467E-4	7.18342639861312E-4
18	0.0	0.0	0.0	4.7889509324087467E-4	8.380664131715307E-4
19	0.0	0.0	0.0	5.986188665510933E-4	8.380664131715307E-4
20	0.0	0.0	0.0	7.18342639861312E-4	0.0011972377331021867
21	0.0	0.0	0.0	0.0011972377331021867	0.0011972377331021867
22	0.0	0.0	0.0	0.0016761328263430614	0.0011972377331021867
23	0.0	0.0	0.0	0.0022747516928941546	0.0011972377331021867
24	0.0	0.0	0.0	0.003112818106065685	0.0011972377331021867
25	0.0	0.0	0.0	0.003950884519237216	0.0011972377331021867
26	0.0	0.0	0.0	0.004190332065857653	0.0011972377331021867
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	140	0.0	19.426027	29
CTAGCGG	220	0.0	18.179436	29
GTAAACG	125	1.4733814E-10	17.917652	27
GTATTAG	170	0.0	17.897156	1
GTCGTAC	45	0.008806229	17.792494	1
CGCCGGT	240	0.0	17.331062	7
CGGTCCA	260	0.0	17.23264	10
TCTAGCG	235	0.0	17.019049	28
CGCTTCG	125	2.8539944E-9	16.637821	32
CGCGGTT	245	0.0	16.328302	10
GCCGGTC	255	0.0	16.311588	8
CCGGTCC	310	0.0	16.001738	9
CGAACGA	100	1.3118297E-6	16.001736	16
CATCGTT	170	3.6379788E-12	15.997906	28
TAGCGGC	250	0.0	15.997905	30
AAACGCT	130	4.802132E-9	15.997905	29
TAGGACA	150	1.1459633E-10	15.995989	4
AGGACCG	355	0.0	15.771638	5
ATACCGT	165	2.910383E-11	15.512191	6
CTTGCGC	290	0.0	15.446253	3
>>END_MODULE