FastQCFastQC Report
Thu 2 Feb 2017
SRR4062996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835256
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29760.3562979493712108No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA26650.31906385587173275No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC25110.30062639478195907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18670.22352428477017827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12440.148936373997912No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG11700.14007681477295583No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10470.12535079065579893No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9630.11529399369774057No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.10571609183292308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAC653.8128928E-722.1544693
CCCTATA555.8322727E-520.3629882
GATCGAA400.00448174420.0005611
CGCGCAA1052.7830538E-1019.80889321
TACCGTC1500.019.2016897
GCGCAAA1105.2023097E-1018.9084922
TATGCAC802.7935785E-618.0005043
TACACCG450.00884382317.779345
CGTCTTA904.364647E-717.77721215
ACCGTCG1550.017.549938
GGGTATA550.001362871717.4571271
CGCAAAT1201.6570993E-917.33278323
CTAGCGG1201.6570993E-917.33278329
ATACCGT1601.8189894E-1217.0014956
CGAGCCG2100.016.7613715
GCGCGCA1151.839362E-816.6951220
TAGCGGC1252.8503564E-916.63947130
CGAACCC1354.4201443E-1016.59305810
GTATTAG1950.016.4126821
AGGCCCG2150.016.37255310
Produced by FastQC (version 0.11.5)