##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062996_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835256
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.150562222839465	32.0	32.0	32.0	32.0	32.0
2	31.233085425306733	32.0	32.0	32.0	32.0	32.0
3	31.345316884883196	32.0	32.0	32.0	32.0	32.0
4	31.4606276399092	32.0	32.0	32.0	32.0	32.0
5	31.355656229946266	32.0	32.0	32.0	32.0	32.0
6	34.88777213213673	36.0	36.0	36.0	36.0	36.0
7	34.92999152355685	36.0	36.0	36.0	36.0	36.0
8	34.858716369592074	36.0	36.0	36.0	32.0	36.0
9	34.98048143323724	36.0	36.0	36.0	36.0	36.0
10	34.80806603005546	36.0	36.0	36.0	32.0	36.0
11	34.99822808815501	36.0	36.0	36.0	36.0	36.0
12	34.89107052209143	36.0	36.0	36.0	32.0	36.0
13	34.96317416456751	36.0	36.0	36.0	36.0	36.0
14	34.89320759144502	36.0	36.0	36.0	32.0	36.0
15	34.85659965327995	36.0	36.0	36.0	32.0	36.0
16	34.868642667637225	36.0	36.0	36.0	32.0	36.0
17	34.8311284205082	36.0	36.0	36.0	32.0	36.0
18	34.83227297978105	36.0	36.0	36.0	32.0	36.0
19	34.82263042707864	36.0	36.0	36.0	32.0	36.0
20	34.8071561293783	36.0	36.0	36.0	32.0	36.0
21	34.797124474412634	36.0	36.0	36.0	32.0	36.0
22	34.775185092953535	36.0	36.0	36.0	32.0	36.0
23	34.69990877048474	36.0	36.0	36.0	32.0	36.0
24	34.68953829724061	36.0	36.0	36.0	32.0	36.0
25	34.65182411141016	36.0	36.0	36.0	32.0	36.0
26	34.59212385184902	36.0	36.0	36.0	32.0	36.0
27	34.558498232877106	36.0	36.0	36.0	32.0	36.0
28	34.53117966228318	36.0	36.0	36.0	32.0	36.0
29	34.4865454423554	36.0	36.0	36.0	32.0	36.0
30	34.457125719539874	36.0	36.0	36.0	32.0	36.0
31	34.44316353309644	36.0	36.0	36.0	32.0	36.0
32	34.41569171607268	36.0	36.0	36.0	32.0	36.0
33	34.38202419378011	36.0	36.0	36.0	32.0	36.0
34	34.36206863524476	36.0	36.0	36.0	32.0	36.0
35	34.29721785895582	36.0	36.0	36.0	32.0	36.0
36	34.27370889882862	36.0	36.0	36.0	32.0	36.0
37	34.24246578294559	36.0	36.0	36.0	32.0	36.0
38	33.80715732661603	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	13.0
22	66.0
23	196.0
24	511.0
25	1330.0
26	2931.0
27	5581.0
28	10033.0
29	16067.0
30	24583.0
31	35891.0
32	51688.0
33	82435.0
34	195287.0
35	408642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9275164667271	17.633796273568585	11.831377840892083	26.607309418812243
2	15.499319968967598	20.189019893302174	37.09605198885132	27.215608148878907
3	18.788970088212476	24.131882919727605	27.911203271811274	29.16794372024864
4	12.043627395226606	15.893948554633017	35.52519889135653	36.53722515878384
5	13.86892162402904	37.286173340867954	33.253756932006475	15.591148103096536
6	35.00870991493616	35.14088511891578	16.365900234060256	13.484504732087805
7	30.595400435556652	30.261968529295913	20.220699596409712	18.921931438737722
8	28.53728941605796	33.18507202147414	18.79873616709988	19.47890239536802
9	27.323307244429916	14.302561836926994	17.900651444348263	40.47347947429483
10	15.746529731645351	26.912089727718325	31.629188327159447	25.712192213476875
11	37.915428472909	21.098462444715295	21.48147438645055	19.504634695925155
12	24.985393699656154	23.611084505828153	28.44876301397416	22.954758780541535
13	30.236383750053875	19.422282137606377	24.730261046965467	25.611073065374285
14	23.438442824714816	19.677081038627676	24.87883954141006	32.005636595247445
15	25.316669380405525	27.25571561293783	22.526027948317644	24.901587058339
16	25.69529747837185	25.739715104626853	24.116496299329306	24.44849111767199
17	24.018265058856205	26.08900744202975	25.329958719242963	24.56276877987108
18	25.164739912074864	24.319490072504717	26.940363194038714	23.57540682138171
19	25.400356297949372	25.49386056490465	25.358692424837415	23.747090712308562
20	25.588562069593035	24.00772936680491	26.058477879835646	24.345230683766413
21	26.209329834206518	24.450467880506096	24.37097129502811	24.969230990259277
22	25.39341231909738	24.89081191874108	25.12798471366862	24.587791048492917
23	23.82481996516034	24.617631741204782	25.774643671693077	25.782904621941803
24	24.411078759087033	25.360847452757003	25.48164874002701	24.746425048128955
25	24.98976359287115	24.547502915280862	25.288475122537573	25.174258369310415
26	23.974086986504737	25.465725478176747	25.965332784200296	24.59485475111822
27	25.257675799606112	25.08443529221615	25.276113282769934	24.38177562540781
28	24.274593657513385	24.619278400873505	25.821065637361478	25.285062304251632
29	24.101353357533498	25.249145172258565	26.037885390826286	24.61161607938165
30	24.448911471453062	24.86794467803883	26.26368442728936	24.41945942321875
31	24.608982156368825	25.019634698822873	25.222446770810386	25.14893637399791
32	24.31685614949189	25.26782208089496	25.183656268257877	25.231665501355273
33	23.724702366699553	24.91056634133727	25.964494717787122	25.40023657417606
34	24.511167833574376	25.252856609231184	26.097867001254706	24.138108555939734
35	25.278597220492877	24.9953307728409	25.92702117674102	23.799050829925196
36	24.168398670587223	25.555159136839485	25.63369793213099	24.642744260442306
37	25.18054345015181	25.3679111553823	25.225200417596522	24.226344976869367
38	24.34040943283053	25.26420138568793	25.316640586744377	25.078748594737167
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	42.5
2	62.0
3	62.0
4	225.5
5	389.0
6	389.0
7	567.0
8	745.0
9	778.5
10	812.0
11	812.0
12	1020.5
13	1229.0
14	1623.5
15	2018.0
16	2018.0
17	2991.5
18	3965.0
19	3965.0
20	4652.5
21	5340.0
22	5474.0
23	5608.0
24	5608.0
25	6548.0
26	7488.0
27	7488.0
28	9985.0
29	12482.0
30	15860.5
31	19239.0
32	19239.0
33	24502.0
34	29765.0
35	29765.0
36	33157.5
37	36550.0
38	41510.5
39	46471.0
40	46471.0
41	49640.5
42	52810.0
43	58690.5
44	64571.0
45	64571.0
46	71461.5
47	78352.0
48	78352.0
49	79891.5
50	81431.0
51	79037.0
52	76643.0
53	76643.0
54	72879.0
55	69115.0
56	69115.0
57	67113.0
58	65111.0
59	58900.0
60	52689.0
61	52689.0
62	48397.5
63	44106.0
64	36902.0
65	29698.0
66	29698.0
67	24737.5
68	19777.0
69	19777.0
70	15786.0
71	11795.0
72	9248.5
73	6702.0
74	6702.0
75	5105.5
76	3509.0
77	3509.0
78	3222.0
79	2935.0
80	2311.0
81	1687.0
82	1687.0
83	1525.5
84	1364.0
85	1364.0
86	901.0
87	438.0
88	341.5
89	245.0
90	245.0
91	149.0
92	53.0
93	37.5
94	22.0
95	22.0
96	16.0
97	10.0
98	10.0
99	8.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010415968277989024
2	0.0
3	0.0
4	1.1972377331021867E-4
5	0.0
6	1.1972377331021867E-4
7	3.59171319930656E-4
8	0.0022747516928941546
9	0.004190332065857653
10	0.002155027919583936
11	0.004070608292547435
12	0.0
13	4.7889509324087467E-4
14	0.0
15	0.0
16	2.3944754662043734E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.1972377331021867E-4
24	0.0
25	2.3944754662043734E-4
26	0.0
27	1.1972377331021867E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.59171319930656E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	835256.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.8041408319553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93403846797686	46.41648314505888
2	13.45099158233901	15.81948006674617
3	3.8966001493101796	6.874086718375614
4	1.4647533157189012	3.4453424104643102
5	0.6921242278199011	2.034988528296489
6	0.36812786436726475	1.2988465668251745
7	0.20642656240825075	0.8497115653117832
8	0.14783175918573457	0.695449566927491
9	0.10799612489564563	0.5715557403902082
>10	0.5754193468407068	6.5028394902515885
>50	0.07437300217622866	3.0997165948050536
>100	0.07477949872391025	8.761047456888157
>500	0.005107889247971081	2.0108838610333386
>1k	0.0014302089894319026	1.6195682886257632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2976	0.3562979493712108	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2665	0.31906385587173275	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2511	0.30062639478195907	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1867	0.22352428477017827	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1244	0.148936373997912	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1170	0.14007681477295583	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1047	0.12535079065579893	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	963	0.11529399369774057	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	883	0.10571609183292308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.1972377331021867E-4	0.0
10	0.0	0.0	0.0	1.1972377331021867E-4	0.0
11	0.0	0.0	0.0	2.3944754662043734E-4	0.0
12	0.0	0.0	0.0	2.3944754662043734E-4	1.1972377331021867E-4
13	0.0	0.0	0.0	2.3944754662043734E-4	1.1972377331021867E-4
14	0.0	0.0	0.0	2.3944754662043734E-4	1.1972377331021867E-4
15	0.0	0.0	0.0	3.59171319930656E-4	4.7889509324087467E-4
16	0.0	0.0	0.0	3.59171319930656E-4	5.986188665510933E-4
17	0.0	0.0	0.0	4.7889509324087467E-4	5.986188665510933E-4
18	0.0	0.0	0.0	5.986188665510933E-4	7.18342639861312E-4
19	0.0	0.0	0.0	7.18342639861312E-4	7.18342639861312E-4
20	0.0	0.0	0.0	9.577901864817493E-4	9.577901864817493E-4
21	0.0	0.0	0.0	0.001436685279722624	0.001077513959791968
22	0.0	0.0	0.0	0.0019155803729634987	0.001077513959791968
23	0.0	0.0	0.0	0.002514199239514592	0.001077513959791968
24	0.0	0.0	0.0	0.00359171319930656	0.001077513959791968
25	0.0	0.0	0.0	0.004549503385788309	0.001077513959791968
26	0.0	0.0	0.0	0.0046692271590985275	0.0011972377331021867
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGAC	65	3.8128928E-7	22.154469	3
CCCTATA	55	5.8322727E-5	20.362988	2
GATCGAA	40	0.004481744	20.00056	11
CGCGCAA	105	2.7830538E-10	19.808893	21
TACCGTC	150	0.0	19.201689	7
GCGCAAA	110	5.2023097E-10	18.90849	22
TATGCAC	80	2.7935785E-6	18.000504	3
TACACCG	45	0.008843823	17.77934	5
CGTCTTA	90	4.364647E-7	17.777212	15
ACCGTCG	155	0.0	17.54993	8
GGGTATA	55	0.0013628717	17.457127	1
CGCAAAT	120	1.6570993E-9	17.332783	23
CTAGCGG	120	1.6570993E-9	17.332783	29
ATACCGT	160	1.8189894E-12	17.001495	6
CGAGCCG	210	0.0	16.76137	15
GCGCGCA	115	1.839362E-8	16.69512	20
TAGCGGC	125	2.8503564E-9	16.639471	30
CGAACCC	135	4.4201443E-10	16.593058	10
GTATTAG	195	0.0	16.412682	1
AGGCCCG	215	0.0	16.372553	10
>>END_MODULE