FastQCFastQC Report
Thu 2 Feb 2017
SRR4062995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104370
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48750.15703669343538304No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG38020.12247251455206691No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA37800.12176383614066623No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC36390.11722185177668899No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG35050.11290535599815744No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG34740.11190676369118371No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC34520.11119808527978302No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA33540.10804124508354351No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC32830.10575414657402306No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG31800.10243624310246524No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT31720.10217854186195588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46500.019.6334721
ACCGTCG8000.015.5974958
TCTAGCG8200.015.41389728
TAGTACG859.414475E-515.0636842
CGTCGTA8100.015.01356310
CGCTTCG7250.014.78526132
GTCCTAC3900.014.784721
CTAGCGG8450.014.76852429
CCGTCGT8600.014.6986299
ATACCGT8650.014.6094286
AACGCTT7350.014.58410230
AACGCAG61750.014.5068586
TAAACGC7400.014.269587528
TCAACGC63150.014.2610114
TATCAAC64150.014.221272
AAACGCT7450.014.17381929
TACCGTC8600.014.1372657
GGTATCA18250.014.1298721
GTCCTAT11350.014.1117021
CAACGCA64150.013.9636725
Produced by FastQC (version 0.11.5)