##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062995_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3104370
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251447797781836	32.0	32.0	32.0	32.0	32.0
2	30.787490859659126	32.0	32.0	32.0	32.0	32.0
3	30.836087515341276	32.0	32.0	32.0	32.0	32.0
4	30.92400261566759	32.0	32.0	32.0	32.0	32.0
5	30.784531805164978	32.0	32.0	32.0	32.0	32.0
6	34.4704787122669	36.0	36.0	36.0	32.0	36.0
7	34.406019256725195	36.0	36.0	36.0	32.0	36.0
8	34.38898520472753	36.0	36.0	36.0	32.0	36.0
9	34.56241008642655	36.0	36.0	36.0	32.0	36.0
10	34.22213460380045	36.0	36.0	36.0	32.0	36.0
11	34.54281802749028	36.0	36.0	36.0	32.0	36.0
12	34.33554827549551	36.0	36.0	36.0	32.0	36.0
13	34.43701588406021	36.0	36.0	36.0	32.0	36.0
14	34.32735981857833	36.0	36.0	36.0	32.0	36.0
15	34.244349417112005	36.0	36.0	36.0	32.0	36.0
16	34.280661454659075	36.0	36.0	36.0	32.0	36.0
17	34.1976996943019	36.0	36.0	36.0	32.0	36.0
18	34.220524937426916	36.0	36.0	36.0	32.0	36.0
19	34.21682950163801	36.0	36.0	36.0	32.0	36.0
20	34.20291363465051	36.0	36.0	36.0	32.0	36.0
21	34.19744134880829	36.0	36.0	36.0	32.0	36.0
22	34.17138324362109	36.0	36.0	36.0	32.0	36.0
23	34.10102855007618	36.0	36.0	36.0	32.0	36.0
24	34.08160915097105	36.0	36.0	36.0	32.0	36.0
25	34.06269613480352	36.0	36.0	36.0	32.0	36.0
26	34.01426118664979	36.0	36.0	36.0	32.0	36.0
27	34.02567477459195	36.0	36.0	36.0	32.0	36.0
28	34.002698454114686	36.0	36.0	36.0	32.0	36.0
29	33.97062302496159	36.0	36.0	36.0	32.0	36.0
30	33.945199186952586	36.0	36.0	36.0	32.0	36.0
31	33.96499901751402	36.0	36.0	36.0	32.0	36.0
32	33.91745378289315	36.0	36.0	36.0	32.0	36.0
33	33.87957170053827	36.0	36.0	36.0	32.0	36.0
34	33.900424240667185	36.0	36.0	36.0	32.0	36.0
35	33.86618251046106	36.0	36.0	36.0	32.0	36.0
36	33.81510966798417	36.0	36.0	36.0	32.0	36.0
37	33.82037740346673	36.0	36.0	36.0	32.0	36.0
38	33.218312894403695	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	6.0
9	7.0
10	31.0
11	25.0
12	17.0
13	15.0
14	300.0
15	621.0
16	894.0
17	1041.0
18	1275.0
19	1694.0
20	2347.0
21	3574.0
22	5579.0
23	8790.0
24	13952.0
25	20923.0
26	31092.0
27	44949.0
28	63025.0
29	85422.0
30	114893.0
31	156403.0
32	211796.0
33	317679.0
34	692126.0
35	1325887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3231275514186	17.135954611919917	11.359146212854666	26.18177162380681
2	17.0232857948594	19.661010394005316	37.154040770284425	26.161663040850858
3	18.861585785060704	24.032007711335236	28.212095224165452	28.894311279438607
4	12.929869519068	15.316377201191255	35.98033041424998	35.773422865490765
5	15.088119527258895	36.06862341807321	33.31066631060865	15.532590744059249
6	34.06585264893996	35.31725591950663	17.013711382879855	13.603180048673558
7	30.397149824279968	30.051830161997444	21.190805219738625	18.360214793983964
8	28.126556279913594	32.23755121026634	19.72894234492285	19.906950164897218
9	27.85939775026092	14.349946526820343	18.459328170701852	39.331327552216884
10	16.139982521351428	26.02811079517543	31.132997182349076	26.698909501124064
11	37.49187420070805	21.217210909992883	22.378209650453723	18.912705238845348
12	25.499476693449953	23.330452382325284	28.173220427542578	22.996850496682182
13	29.33377793242094	19.181647722879454	25.236700688162067	26.24787365653754
14	24.18684545907907	19.53384637786864	24.718113298480322	31.561194864571963
15	25.430930877714157	26.510763203087993	22.126638806816533	25.931667112381305
16	26.289541753636968	25.426405487835325	23.13509313218401	25.148959626343697
17	24.60706213642595	25.39047309129162	24.721698211992972	25.280766560289457
18	25.56298669900132	24.36667318471118	25.502939079692922	24.56740103659458
19	26.05844608111913	24.576032029627665	24.99792212042939	24.367599768823816
20	26.221160434624196	23.94466334149325	24.32447146590795	25.5097047579746
21	27.40257259317508	23.879423345853	24.025967541217767	24.69203651975415
22	26.383778340322912	23.79833293923675	24.440925136897558	25.376963583542782
23	24.74865338522203	23.597201103064354	25.1805687997732	26.473576711940417
24	25.267087701030594	24.627642968959158	24.64249504581753	25.46277428419272
25	25.2910739887559	24.089469529777535	24.75722087085394	25.86223561061263
26	25.141118613094005	24.91191363283128	25.16457394422013	24.78239380985459
27	25.893897816502164	24.2674693035216	24.269982107546724	25.568650772429514
28	24.995675204473432	24.25509625206938	25.20896735480043	25.54026118865676
29	25.054260299308734	24.499334572372142	25.05036212729368	25.396043001025443
30	24.89179441518731	24.685981529992823	25.382182512121986	25.040041542697878
31	25.723580912913864	24.40685468205478	24.05646002498539	25.813104380045964
32	25.326463027272904	24.421264230811357	24.011429749563494	26.24084299235225
33	24.933322294209194	23.95851823597803	24.819376626673467	26.28878284313931
34	25.73977555282215	24.126686301660254	24.835743248511104	25.297794897006494
35	26.42240472492341	23.982266072700597	24.845475712503156	24.749853489872837
36	25.123207939433595	24.731056004077452	24.423249596716904	25.72248645977205
37	26.1327701161571	24.43786742060107	24.38912499452332	25.04023746871851
38	25.172662512479153	24.194851002375188	24.929409630958542	25.703076854187117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	231.0
1	253.5
2	276.0
3	276.0
4	613.5
5	951.0
6	951.0
7	1114.0
8	1277.0
9	1229.5
10	1182.0
11	1182.0
12	1709.0
13	2236.0
14	3169.5
15	4103.0
16	4103.0
17	6234.5
18	8366.0
19	8366.0
20	11046.0
21	13726.0
22	15599.0
23	17472.0
24	17472.0
25	21786.0
26	26100.0
27	26100.0
28	33296.0
29	40492.0
30	50417.0
31	60342.0
32	60342.0
33	79488.5
34	98635.0
35	98635.0
36	111458.0
37	124281.0
38	143803.0
39	163325.0
40	163325.0
41	176934.5
42	190544.0
43	217942.0
44	245340.0
45	245340.0
46	251256.0
47	257172.0
48	257172.0
49	276137.5
50	295103.0
51	300119.5
52	305136.0
53	305136.0
54	291124.0
55	277112.0
56	277112.0
57	268687.5
58	260263.0
59	233878.5
60	207494.0
61	207494.0
62	194509.0
63	181524.0
64	150013.0
65	118502.0
66	118502.0
67	98807.5
68	79113.0
69	79113.0
70	63709.5
71	48306.0
72	37672.0
73	27038.0
74	27038.0
75	20455.5
76	13873.0
77	13873.0
78	13161.5
79	12450.0
80	9743.0
81	7036.0
82	7036.0
83	7164.0
84	7292.0
85	7292.0
86	4879.0
87	2466.0
88	2199.5
89	1933.0
90	1933.0
91	1406.0
92	879.0
93	754.5
94	630.0
95	630.0
96	590.0
97	550.0
98	550.0
99	1084.5
100	1619.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11738291505200733
2	0.05218450120314267
3	0.01185425706342994
4	0.0027380756804118067
5	2.254885854456782E-4
6	4.831898259550247E-4
7	0.0
8	3.8655186076401975E-4
9	3.221265506366831E-4
10	3.5433920570035146E-4
11	0.001932759303820099
12	0.00228709850952045
13	0.014495694778650741
14	0.009212819348209137
15	0.02383736474711455
16	0.010984515376710895
17	0.02045503596542938
18	0.005379513395632608
19	0.00785988783553507
20	0.005508364015887281
21	0.0060237664969059745
22	0.007215634734261702
23	0.009341669968463811
24	0.01375480371218637
25	0.016750580633107522
26	0.01926316772807365
27	0.008632991557063107
28	0.005701639946269292
29	0.011306641927347578
30	0.0030279895759848215
31	0.005025174189932257
32	0.006120404462096979
33	0.008343077661490093
34	0.012498510164703306
35	0.015751988326133805
36	0.015172160534987777
37	0.009599371208973157
38	0.006217042427287984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3104370.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.72874022537843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10672933034994	41.43820191834375
2	11.501874102477085	11.8995491510409
3	3.46375148373411	5.375265021220527
4	1.4830077026102106	3.068564808022354
5	0.8275853916469065	2.140497486941045
6	0.5192652928853163	1.6116563666231747
7	0.36417423884679373	1.31867922186565
8	0.2685620229691263	1.111390009645764
9	0.19239253210015017	0.8957000982880304
>10	1.1212417867729123	10.294517480270276
>50	0.0702721788170864	2.4988452776736776
>100	0.06145402438797788	6.9391926077853
>500	0.011813947441515964	4.256503607645869
>1k	0.007875964960845354	7.1514369446338835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4875	0.15703669343538304	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3802	0.12247251455206691	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3780	0.12176383614066623	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3639	0.11722185177668899	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3505	0.11290535599815744	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3474	0.11190676369118371	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3452	0.11119808527978302	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3354	0.10804124508354351	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3283	0.10575414657402306	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3180	0.10243624310246524	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3172	0.10217854186195588	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.663796519100494E-5
2	0.0	0.0	0.0	0.0	9.663796519100494E-5
3	0.0	0.0	0.0	0.0	9.663796519100494E-5
4	0.0	0.0	0.0	0.0	9.663796519100494E-5
5	0.0	0.0	0.0	0.0	1.2885062025467324E-4
6	0.0	0.0	0.0	0.0	1.9327593038200987E-4
7	0.0	0.0	0.0	0.0	1.9327593038200987E-4
8	0.0	0.0	0.0	0.0	1.9327593038200987E-4
9	0.0	0.0	0.0	3.221265506366831E-5	1.9327593038200987E-4
10	0.0	0.0	0.0	6.442531012733662E-5	1.9327593038200987E-4
11	0.0	0.0	0.0	6.442531012733662E-5	1.9327593038200987E-4
12	0.0	0.0	0.0	6.442531012733662E-5	4.509771708913564E-4
13	0.0	0.0	0.0	6.442531012733662E-5	4.8318982595502467E-4
14	0.0	0.0	0.0	6.442531012733662E-5	4.8318982595502467E-4
15	0.0	0.0	0.0	9.663796519100494E-5	6.12040446209698E-4
16	0.0	0.0	0.0	9.663796519100494E-5	6.442531012733662E-4
17	0.0	0.0	0.0	9.663796519100494E-5	6.442531012733662E-4
18	0.0	0.0	0.0	1.6106327531834156E-4	6.442531012733662E-4
19	0.0	0.0	0.0	1.9327593038200987E-4	6.442531012733662E-4
20	0.0	0.0	0.0	1.9327593038200987E-4	6.442531012733662E-4
21	0.0	0.0	0.0	2.254885854456782E-4	6.442531012733662E-4
22	0.0	0.0	0.0	3.5433920570035146E-4	6.442531012733662E-4
23	0.0	0.0	0.0	4.8318982595502467E-4	6.442531012733662E-4
24	0.0	0.0	0.0	7.731037215280395E-4	6.442531012733662E-4
25	0.0	0.0	0.0	9.019543417827128E-4	6.442531012733662E-4
26	0.0	0.0	0.0	0.0011596555822920593	6.764657563370346E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4650	0.0	19.633472	1
ACCGTCG	800	0.0	15.597495	8
TCTAGCG	820	0.0	15.413897	28
TAGTACG	85	9.414475E-5	15.063684	2
CGTCGTA	810	0.0	15.013563	10
CGCTTCG	725	0.0	14.785261	32
GTCCTAC	390	0.0	14.78472	1
CTAGCGG	845	0.0	14.768524	29
CCGTCGT	860	0.0	14.698629	9
ATACCGT	865	0.0	14.609428	6
AACGCTT	735	0.0	14.584102	30
AACGCAG	6175	0.0	14.506858	6
TAAACGC	740	0.0	14.2695875	28
TCAACGC	6315	0.0	14.261011	4
TATCAAC	6415	0.0	14.22127	2
AAACGCT	745	0.0	14.173819	29
TACCGTC	860	0.0	14.137265	7
GGTATCA	1825	0.0	14.129872	1
GTCCTAT	1135	0.0	14.111702	1
CAACGCA	6415	0.0	13.963672	5
>>END_MODULE