FastQCFastQC Report
Thu 2 Feb 2017
SRR4062995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104370
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51430.16566968499244614No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA39760.12807751653314522No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG39750.12804530387808152No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC39020.12569378005843376No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC36620.11796274284315336No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA35520.11441935078614984No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC34740.11190676369118371No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG34020.1095874525265996No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG33550.1080734577386072No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG32410.104401215061349No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32280.10398245054552133No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC31280.10076118503915447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG400.00448340420.001369
GTATCAA41400.019.2869571
TACCGTC8300.015.037417
ATACCGT9100.014.9463116
GGTATCA16600.014.8448611
TATTAGG2050.014.82908252
ACCGTCG8100.014.816068
GGACCGT2200.014.5466786
GTTTTCG11000.014.39958728
TTTTCGG11150.014.34936429
TACACCG1452.0314474E-814.3460335
CGTCGTA8300.014.07324610
ATCGTTT8100.013.82676329
CATCGTT8100.013.82676328
TCTAGCG8300.013.68635528
CGTTTAT8350.013.604431
CTAGCGG8650.013.50250329
TATACCG952.4273255E-413.4748185
GTATTAG6100.013.3783881
CGGCATC8850.013.37814725
Produced by FastQC (version 0.11.5)