##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3104370
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218322880326767	32.0	32.0	32.0	32.0	32.0
2	31.250730099827017	32.0	32.0	32.0	32.0	32.0
3	31.346120468887406	32.0	32.0	32.0	32.0	32.0
4	31.45759719363349	32.0	32.0	32.0	32.0	32.0
5	31.369508789222934	32.0	32.0	32.0	32.0	32.0
6	34.899663377754585	36.0	36.0	36.0	36.0	36.0
7	34.92635124034828	36.0	36.0	36.0	36.0	36.0
8	34.863064969704	36.0	36.0	36.0	32.0	36.0
9	34.9793339711439	36.0	36.0	36.0	36.0	36.0
10	34.811340143088614	36.0	36.0	36.0	32.0	36.0
11	35.00570163994627	36.0	36.0	36.0	36.0	36.0
12	34.89226026536785	36.0	36.0	36.0	32.0	36.0
13	34.951448119908385	36.0	36.0	36.0	36.0	36.0
14	34.897643966408644	36.0	36.0	36.0	32.0	36.0
15	34.864804775203986	36.0	36.0	36.0	32.0	36.0
16	34.88319079233467	36.0	36.0	36.0	32.0	36.0
17	34.84453979390343	36.0	36.0	36.0	32.0	36.0
18	34.84672091277779	36.0	36.0	36.0	32.0	36.0
19	34.84418899808979	36.0	36.0	36.0	32.0	36.0
20	34.827920318776435	36.0	36.0	36.0	32.0	36.0
21	34.824916166565195	36.0	36.0	36.0	32.0	36.0
22	34.80749298569436	36.0	36.0	36.0	32.0	36.0
23	34.75178184301485	36.0	36.0	36.0	32.0	36.0
24	34.729160828122936	36.0	36.0	36.0	32.0	36.0
25	34.7103705421712	36.0	36.0	36.0	32.0	36.0
26	34.641815891791246	36.0	36.0	36.0	32.0	36.0
27	34.62648975476506	36.0	36.0	36.0	32.0	36.0
28	34.59987308213905	36.0	36.0	36.0	32.0	36.0
29	34.56661190515306	36.0	36.0	36.0	32.0	36.0
30	34.54231454369163	36.0	36.0	36.0	32.0	36.0
31	34.547698566858976	36.0	36.0	36.0	32.0	36.0
32	34.52136568772408	36.0	36.0	36.0	32.0	36.0
33	34.49069086481315	36.0	36.0	36.0	32.0	36.0
34	34.488335797601444	36.0	36.0	36.0	32.0	36.0
35	34.4580323866034	36.0	36.0	36.0	32.0	36.0
36	34.43681584347227	36.0	36.0	36.0	32.0	36.0
37	34.434006577824164	36.0	36.0	36.0	32.0	36.0
38	33.98888341273753	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	11.0
21	52.0
22	225.0
23	773.0
24	2012.0
25	4961.0
26	10518.0
27	20697.0
28	35666.0
29	56544.0
30	86035.0
31	124882.0
32	181295.0
33	293310.0
34	717200.0
35	1570187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.582738765823144	18.3765737770036	12.113101575766324	26.927585881406934
2	15.944201238898714	20.740053537432715	36.3629657547264	26.95277946894217
3	17.832539291386013	24.926603465437434	28.43594674603865	28.804910497137904
4	12.3896350972068	16.29926416418709	35.933578601200054	35.377522137406054
5	14.522477529235427	36.677042372889545	32.92896748354818	15.87151261432685
6	34.086208962608765	35.76871140111147	16.65867791277054	13.486401723509228
7	30.16219124070662	30.272487726938884	21.099292607816103	18.46602842453839
8	28.07353522910742	32.46578625574524	19.48141099401219	19.979267521135142
9	27.69379448531129	14.204342104627358	18.229687960557364	39.872175449503985
10	15.867661360437763	26.312912346235386	31.18125952094794	26.63816677237892
11	37.6781522994782	21.14981319014788	22.244643270174272	18.92739124019965
12	25.294698614012944	23.414921861460567	28.098209033880757	23.19217049064573
13	29.324102448136667	19.199216843659613	25.185891718134584	26.29078899006913
14	24.043654590142282	19.47390291750017	24.65376227704816	31.828680215309387
15	25.39900205194613	26.637643064454302	21.921227173307305	26.042127710292267
16	26.26278602458602	25.49528631611092	23.05556110329774	25.186366556005318
17	24.544496951072198	25.5515289736726	24.515247860274386	25.388726214980817
18	25.618338020274646	24.454623643444563	25.39632840157584	24.530709934704948
19	26.01542341924448	24.604154788250113	24.949377812567445	24.431043979937957
20	26.16820804221146	23.94563148078354	24.326191787705717	25.55996868929928
21	27.381855103425305	23.944760379414028	23.92485293293381	24.74853158422686
22	26.25461084039945	23.92734240033965	24.424416040747733	25.393630718513165
23	24.67539286579426	23.569853831762213	25.168826633955767	26.585926668487758
24	25.21442353843131	24.597325705376612	24.661751015503953	25.526499740688124
25	25.32650768207893	24.046311519768274	24.85362560108853	25.773555197064262
26	25.223266056086135	24.82257258160115	25.0871352880844	24.867026074228313
27	25.887162108837074	24.239514361230075	24.21406632274233	25.65925720719052
28	24.951761549042157	24.256258113562495	25.17592941563023	25.616050921765126
29	25.068049233822	24.404243050924986	25.072494580220784	25.455213135032228
30	24.926474614817177	24.518565763745944	25.351810512277854	25.203149109159025
31	25.653417601638978	24.30296646340481	24.157687389067668	25.885928545888536
32	25.37542238843952	24.318428537835374	24.09103940574094	26.215109667984166
33	24.844976597506097	23.969211144290146	24.91094811507649	26.274864143127267
34	25.663532375328973	24.052029880458836	24.998276622954094	25.2861611212581
35	26.392085994903958	23.91084181331478	24.848745478148544	24.84832671363272
36	25.18040697468407	24.73451940329278	24.433041164551906	25.65203245747124
37	26.168594594072225	24.3732866894088	24.41216736407065	25.04595135244832
38	25.190980840228722	24.070914250206723	24.94236348836108	25.795741421203473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	131.0
2	166.0
3	166.0
4	515.5
5	865.0
6	865.0
7	1048.0
8	1231.0
9	1147.5
10	1064.0
11	1064.0
12	1535.0
13	2006.0
14	2979.0
15	3952.0
16	3952.0
17	6259.5
18	8567.0
19	8567.0
20	11104.0
21	13641.0
22	15558.0
23	17475.0
24	17475.0
25	21824.0
26	26173.0
27	26173.0
28	33615.5
29	41058.0
30	50908.5
31	60759.0
32	60759.0
33	80064.0
34	99369.0
35	99369.0
36	112314.5
37	125260.0
38	144430.5
39	163601.0
40	163601.0
41	177377.5
42	191154.0
43	218828.0
44	246502.0
45	246502.0
46	251914.0
47	257326.0
48	257326.0
49	276519.0
50	295712.0
51	300128.5
52	304545.0
53	304545.0
54	290927.5
55	277310.0
56	277310.0
57	269096.5
58	260883.0
59	234340.5
60	207798.0
61	207798.0
62	195040.5
63	182283.0
64	150607.5
65	118932.0
66	118932.0
67	99408.5
68	79885.0
69	79885.0
70	64113.5
71	48342.0
72	37740.5
73	27139.0
74	27139.0
75	20064.0
76	12989.0
77	12989.0
78	12653.5
79	12318.0
80	9527.5
81	6737.0
82	6737.0
83	6412.0
84	6087.0
85	6087.0
86	3831.5
87	1576.0
88	1377.0
89	1178.0
90	1178.0
91	704.0
92	230.0
93	152.5
94	75.0
95	75.0
96	62.0
97	49.0
98	49.0
99	43.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009438307933654817
2	0.0
3	0.0
4	2.899138955730148E-4
5	9.663796519100494E-5
6	1.9327593038200987E-4
7	3.221265506366831E-4
8	0.0019971846139474354
9	0.0037044553323218564
10	9.985923069737177E-4
11	0.004638622329168237
12	3.8655186076401975E-4
13	2.899138955730148E-4
14	0.0
15	0.0
16	1.2885062025467327E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2885062025467327E-4
22	3.221265506366832E-5
23	6.442531012733663E-5
24	0.0
25	6.442531012733663E-5
26	6.442531012733663E-5
27	1.6106327531834156E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.221265506366832E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3104370.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.53792806619985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.29347830778921	38.7849669646384
2	12.55668844174388	12.440646575535824
3	3.7248337341492856	5.535616367425255
4	1.6388699406238514	3.2474488491392624
5	0.9045586700809051	2.2404981165062634
6	0.5890933842320887	1.7509479415418059
7	0.4021014452004948	1.3943490727359977
8	0.2964452886222982	1.1748228306668207
9	0.22716611179301266	1.0128004654572513
>10	1.2067520820405546	10.661910804642144
>50	0.0744232518504264	2.5678930230365222
>100	0.0633915808904438	6.844481302312985
>500	0.013449231654872192	4.607195517127863
>1k	0.008683241796400152	7.570086796306826
>5k	6.528753230376055E-5	0.16633537292697267
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5143	0.16566968499244614	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3976	0.12807751653314522	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3975	0.12804530387808152	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3902	0.12569378005843376	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3662	0.11796274284315336	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3552	0.11441935078614984	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3474	0.11190676369118371	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3402	0.1095874525265996	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3355	0.1080734577386072	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3241	0.104401215061349	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3228	0.10398245054552133	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3128	0.10076118503915447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.221265506366831E-5
2	0.0	0.0	0.0	0.0	3.221265506366831E-5
3	0.0	0.0	0.0	0.0	3.221265506366831E-5
4	0.0	0.0	0.0	0.0	3.221265506366831E-5
5	0.0	0.0	0.0	0.0	3.221265506366831E-5
6	0.0	0.0	0.0	0.0	6.442531012733662E-5
7	0.0	0.0	0.0	3.221265506366831E-5	6.442531012733662E-5
8	0.0	0.0	0.0	3.221265506366831E-5	6.442531012733662E-5
9	0.0	0.0	0.0	3.221265506366831E-5	6.442531012733662E-5
10	0.0	0.0	0.0	6.442531012733662E-5	6.442531012733662E-5
11	0.0	0.0	0.0	6.442531012733662E-5	6.442531012733662E-5
12	0.0	0.0	0.0	6.442531012733662E-5	1.6106327531834156E-4
13	0.0	0.0	0.0	6.442531012733662E-5	2.254885854456782E-4
14	0.0	0.0	0.0	9.663796519100494E-5	2.254885854456782E-4
15	0.0	0.0	0.0	1.2885062025467324E-4	3.5433920570035146E-4
16	0.0	0.0	0.0	1.2885062025467324E-4	3.8655186076401975E-4
17	3.221265506366831E-5	0.0	0.0	1.6106327531834156E-4	3.8655186076401975E-4
18	3.221265506366831E-5	0.0	0.0	2.254885854456782E-4	4.187645158276881E-4
19	3.221265506366831E-5	0.0	0.0	2.577012405093465E-4	4.8318982595502467E-4
20	3.221265506366831E-5	0.0	0.0	3.221265506366831E-4	5.15402481018693E-4
21	3.221265506366831E-5	0.0	0.0	3.5433920570035146E-4	5.15402481018693E-4
22	3.221265506366831E-5	0.0	0.0	4.8318982595502467E-4	5.476151360823613E-4
23	3.221265506366831E-5	0.0	0.0	6.442531012733662E-4	6.12040446209698E-4
24	3.221265506366831E-5	0.0	0.0	9.019543417827128E-4	6.442531012733662E-4
25	3.221265506366831E-5	0.0	0.0	9.663796519100493E-4	6.442531012733662E-4
26	3.221265506366831E-5	0.0	0.0	0.0012562935474830642	6.442531012733662E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAACG	40	0.004483404	20.00136	9
GTATCAA	4140	0.0	19.286957	1
TACCGTC	830	0.0	15.03741	7
ATACCGT	910	0.0	14.946311	6
GGTATCA	1660	0.0	14.844861	1
TATTAGG	205	0.0	14.8290825	2
ACCGTCG	810	0.0	14.81606	8
GGACCGT	220	0.0	14.546678	6
GTTTTCG	1100	0.0	14.399587	28
TTTTCGG	1115	0.0	14.349364	29
TACACCG	145	2.0314474E-8	14.346033	5
CGTCGTA	830	0.0	14.073246	10
ATCGTTT	810	0.0	13.826763	29
CATCGTT	810	0.0	13.826763	28
TCTAGCG	830	0.0	13.686355	28
CGTTTAT	835	0.0	13.6044	31
CTAGCGG	865	0.0	13.502503	29
TATACCG	95	2.4273255E-4	13.474818	5
GTATTAG	610	0.0	13.378388	1
CGGCATC	885	0.0	13.378147	25
>>END_MODULE