FastQCFastQC Report
Thu 2 Feb 2017
SRR4062994_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062994_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818890
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27640.33753007119393325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18120.22127514073929344No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16550.20210284653616478No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13890.16961985126207427No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13590.16595635555447008No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12990.15862936413926168No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12660.15459951886089707No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12210.14910427529949077No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12020.14678406135134145No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11920.14556289611547338No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11190.1366483898936365No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11040.1348166420398344No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11020.1345724089926608No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11000.13432817594548718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10170.12419250448778224No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9890.12077324182735165No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9720.11869726092637596No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9660.11796456178485512No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9350.1141789495536641No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8980.10966063818095226No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8600.10502021028465362No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT8440.10306634590726471No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT8280.10111248152987581No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT8210.10025766586476816No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG8190.10001343281759456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTATA401.5922626E-424.0016522
TGTATAC350.00206667822.855923
GTACTAT453.515011E-421.3517551
GTGCTAT400.004460059620.0172711
TAGCGGC2650.018.71598230
CTAGCGG2650.018.71598229
TCTAGCG2650.018.71483828
GGACCGT601.13974114E-418.6622496
GTATAGA802.7712485E-618.0155431
GTAAGTC450.00885088517.7768293
TATCCTT550.001366915817.449355
GTCCTAT2300.017.4063221
GCCTAAT652.08567E-417.245651
ATCGTTT2450.016.97868329
CTATTCC2600.016.610444
AGCGGCG3200.016.49911931
ACCGTCG2350.016.3395548
CATCGTT2550.016.31185728
GTAATAA600.00242707716.0138151
CCTAATA600.002439981316.0010992
Produced by FastQC (version 0.11.5)