##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062994_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	818890
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210455616749503	32.0	32.0	32.0	32.0	32.0
2	30.82965233425735	32.0	32.0	32.0	32.0	32.0
3	30.884255516613955	32.0	32.0	32.0	32.0	32.0
4	30.950122727106205	32.0	32.0	32.0	32.0	32.0
5	30.85875392299332	32.0	32.0	32.0	32.0	32.0
6	34.50787651577135	36.0	36.0	36.0	32.0	36.0
7	34.421034571187825	36.0	36.0	36.0	32.0	36.0
8	34.406849515807984	36.0	36.0	36.0	32.0	36.0
9	34.53473482396903	36.0	36.0	36.0	32.0	36.0
10	34.25558988386719	36.0	36.0	36.0	32.0	36.0
11	34.535942556387305	36.0	36.0	36.0	32.0	36.0
12	34.33754228284629	36.0	36.0	36.0	32.0	36.0
13	34.43186874916045	36.0	36.0	36.0	32.0	36.0
14	34.33290307611523	36.0	36.0	36.0	32.0	36.0
15	34.27125377034766	36.0	36.0	36.0	32.0	36.0
16	34.27284616981524	36.0	36.0	36.0	32.0	36.0
17	34.19445224633345	36.0	36.0	36.0	32.0	36.0
18	34.22958761249985	36.0	36.0	36.0	32.0	36.0
19	34.210252903320345	36.0	36.0	36.0	32.0	36.0
20	34.195857807519936	36.0	36.0	36.0	32.0	36.0
21	34.16314767551197	36.0	36.0	36.0	32.0	36.0
22	34.14195557400872	36.0	36.0	36.0	32.0	36.0
23	34.09283298123069	36.0	36.0	36.0	32.0	36.0
24	34.07963218503096	36.0	36.0	36.0	32.0	36.0
25	34.05927536054904	36.0	36.0	36.0	32.0	36.0
26	33.99916472297867	36.0	36.0	36.0	32.0	36.0
27	33.989500421302004	36.0	36.0	36.0	32.0	36.0
28	33.98027818144073	36.0	36.0	36.0	32.0	36.0
29	33.932307147480124	36.0	36.0	36.0	32.0	36.0
30	33.902200539755036	36.0	36.0	36.0	32.0	36.0
31	33.93182234488149	36.0	36.0	36.0	32.0	36.0
32	33.86647290844924	36.0	36.0	36.0	32.0	36.0
33	33.841371857025976	36.0	36.0	36.0	32.0	36.0
34	33.83886114130103	36.0	36.0	36.0	32.0	36.0
35	33.76559489064465	36.0	36.0	36.0	32.0	36.0
36	33.72068531793037	36.0	36.0	36.0	27.0	36.0
37	33.721050446335894	36.0	36.0	36.0	27.0	36.0
38	33.108715456288394	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	0.0
8	3.0
9	3.0
10	7.0
11	14.0
12	1.0
13	2.0
14	144.0
15	267.0
16	335.0
17	467.0
18	539.0
19	650.0
20	795.0
21	1237.0
22	1829.0
23	2667.0
24	3793.0
25	5676.0
26	8348.0
27	11755.0
28	16722.0
29	22389.0
30	29870.0
31	39782.0
32	54287.0
33	81172.0
34	178653.0
35	357481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.005401526071715	17.112861084926713	11.276449810922134	25.605287578079437
2	17.06730886715638	19.809245927598493	36.73763128699129	26.38581391825384
3	19.088748464189667	24.106176705512798	28.100993421968624	28.704081408328918
4	12.759687764084077	15.2635101139622	35.41547599857364	36.561326123380084
5	15.015466076598905	36.08291233610416	32.91251927916971	15.989102308127231
6	34.32528706576838	35.17998165807576	16.69347127632683	13.801259999829036
7	30.584402262580472	30.33100985726986	20.622844638094588	18.46174324205508
8	28.8883922792672	31.663381716136314	19.448861013620945	19.999364990975547
9	27.901495686833854	14.197668057170212	18.468826017721593	39.432010238274344
10	16.521225176642414	25.840471079002448	30.44147781254045	27.196825931814683
11	37.964722375548924	21.092204436324913	21.631358105500713	19.31171508262545
12	25.601107877630326	23.721184129701793	27.59729038923483	23.080417603433055
13	29.80530004995438	18.75035877994057	25.052549810869536	26.391791359235516
14	24.446387658096445	19.70227307145544	24.26661325543109	31.584726015017022
15	25.73781885830922	26.39950748965081	22.433773115433333	25.428900536606637
16	26.48252480187203	25.23311866671389	23.216481901930777	25.0678746294833
17	24.443592133276496	25.302526356162108	24.885151748710438	25.36872976185096
18	25.779064158224795	23.773287658685817	26.109167292555885	24.3384808905335
19	26.072115531401703	24.82777150184716	25.123927579153055	23.976185387598083
20	26.22188244575551	23.301772387490093	24.934816245928687	25.541528920825705
21	27.901111838441473	23.76969898293162	23.645740720838663	24.68344845778824
22	26.384797635621993	23.836007913827213	24.400234483769324	25.378959966781466
23	24.403118233212872	23.51303192366362	25.364528692704052	26.719321150419457
24	25.017404240039276	24.99932825740199	24.791576592091026	25.191690910467702
25	25.33906254580276	23.813403230376878	24.8605153359846	25.987018887835767
26	24.81140833027971	25.060950287040427	25.42091120068401	24.706730181995848
27	25.91697432972605	24.39121795490268	24.369234752511886	25.32257296285938
28	24.776115392929242	23.974002151832416	25.651259041787316	25.598623413451026
29	24.554060507317622	24.588868519755685	25.668405438843543	25.18866553408315
30	24.804362162030138	24.657084239064066	25.760569572332265	24.77798402657353
31	25.39593174634966	24.654384070891567	24.00247671061566	25.947207472143113
32	24.805728194111822	24.660764728080533	24.201082768399186	26.33242430940846
33	24.355440484114236	24.16516551357755	25.136935693654856	26.34245830865336
34	25.501540100002686	24.35055656111913	25.2365109956545	24.91139234322368
35	26.373965945357703	24.043686480372962	25.153314287877883	24.429033286391455
36	24.696701919635935	25.23703845745155	24.500793283411642	25.565466339500876
37	25.953590620420126	24.974230581338546	24.50170981924768	24.57046897899365
38	24.761426725580282	24.690715732035866	25.012273669863927	25.535583872519922
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	626.0
1	431.0
2	236.0
3	236.0
4	550.0
5	864.0
6	864.0
7	973.0
8	1082.0
9	1014.0
10	946.0
11	946.0
12	1206.0
13	1466.0
14	1797.5
15	2129.0
16	2129.0
17	3117.5
18	4106.0
19	4106.0
20	4720.5
21	5335.0
22	5308.0
23	5281.0
24	5281.0
25	5975.0
26	6669.0
27	6669.0
28	8519.0
29	10369.0
30	13224.0
31	16079.0
32	16079.0
33	20627.0
34	25175.0
35	25175.0
36	28056.0
37	30937.0
38	36106.5
39	41276.0
40	41276.0
41	44190.0
42	47104.0
43	54248.5
44	61393.0
45	61393.0
46	65229.0
47	69065.0
48	69065.0
49	72463.0
50	75861.0
51	76160.0
52	76459.0
53	76459.0
54	73084.5
55	69710.0
56	69710.0
57	68744.5
58	67779.0
59	61233.0
60	54687.0
61	54687.0
62	52755.5
63	50824.0
64	41938.0
65	33052.0
66	33052.0
67	27707.5
68	22363.0
69	22363.0
70	18346.0
71	14329.0
72	11167.0
73	8005.0
74	8005.0
75	6024.5
76	4044.0
77	4044.0
78	4095.0
79	4146.0
80	3216.5
81	2287.0
82	2287.0
83	2434.5
84	2582.0
85	2582.0
86	1694.5
87	807.0
88	741.0
89	675.0
90	675.0
91	478.0
92	281.0
93	253.5
94	226.0
95	226.0
96	207.5
97	189.0
98	189.0
99	317.5
100	446.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11881937744996275
2	0.055440901708410165
3	0.012700118453027879
4	0.0026865635189097436
5	1.2211652358680653E-4
6	1.2211652358680653E-4
7	2.4423304717361306E-4
8	4.884660943472261E-4
9	2.4423304717361306E-4
10	4.884660943472261E-4
11	0.0017096313302152916
12	0.0032971461368437763
13	0.017462662872913332
14	0.010502021028465362
15	0.027720450854205082
16	0.01233376888226746
17	0.02454542124094811
18	0.006716408797274359
19	0.007937574033142426
20	0.006227942702927133
21	0.00805969055672923
22	0.008548156651076458
23	0.011356836693573007
24	0.01514244892476401
25	0.01978287682106266
26	0.02320213948149324
27	0.010135671457704942
28	0.0064721757501007456
29	0.013799167165309137
30	0.0036634957076041963
31	0.007571224462382005
32	0.007693340985968812
33	0.00915873926901049
34	0.013677050641722333
35	0.01795112896726056
36	0.01721842982573972
37	0.010990487122812588
38	0.007815457509555618
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	818890.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.484795836481766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24791176164372	43.45527972712001
2	11.660290673011389	12.472965320800935
3	3.3453776930991004	5.367805287339773
4	1.2414084551066227	2.655859110842398
5	0.6429754508843619	1.7194705359209954
6	0.386974800348118	1.241836091428943
7	0.2484966514497863	0.9303554868188836
8	0.15712325252387346	0.6722964065922672
9	0.1314050318245797	0.6325354169121631
>10	0.706789531377706	7.3295350011304965
>50	0.09786190226763365	3.682269146979601
>100	0.11940460302054715	13.428210518907779
>500	0.010542440956749777	3.886345843680779
>1k	0.003437752485896666	2.5252361055249244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2764	0.33753007119393325	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1812	0.22127514073929344	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1655	0.20210284653616478	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1389	0.16961985126207427	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1359	0.16595635555447008	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1299	0.15862936413926168	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1266	0.15459951886089707	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1221	0.14910427529949077	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1202	0.14678406135134145	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1192	0.14556289611547338	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1119	0.1366483898936365	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1104	0.1348166420398344	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1102	0.1345724089926608	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1100	0.13432817594548718	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1017	0.12419250448778224	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	989	0.12077324182735165	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	972	0.11869726092637596	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	966	0.11796456178485512	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	935	0.1141789495536641	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	898	0.10966063818095226	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	860	0.10502021028465362	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	844	0.10306634590726471	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	828	0.10111248152987581	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	821	0.10025766586476816	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	819	0.10001343281759456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.2211652358680653E-4	0.0
6	0.0	0.0	0.0	1.2211652358680653E-4	0.0
7	0.0	0.0	0.0	1.2211652358680653E-4	0.0
8	0.0	0.0	0.0	1.2211652358680653E-4	0.0
9	0.0	0.0	0.0	1.2211652358680653E-4	0.0
10	0.0	0.0	1.2211652358680653E-4	1.2211652358680653E-4	0.0
11	0.0	0.0	1.2211652358680653E-4	1.2211652358680653E-4	0.0
12	0.0	0.0	1.2211652358680653E-4	2.4423304717361306E-4	0.0
13	0.0	0.0	1.2211652358680653E-4	2.4423304717361306E-4	1.2211652358680653E-4
14	0.0	0.0	1.2211652358680653E-4	2.4423304717361306E-4	1.2211652358680653E-4
15	0.0	0.0	1.2211652358680653E-4	2.4423304717361306E-4	2.4423304717361306E-4
16	0.0	0.0	1.2211652358680653E-4	3.663495707604196E-4	2.4423304717361306E-4
17	0.0	0.0	1.2211652358680653E-4	3.663495707604196E-4	2.4423304717361306E-4
18	0.0	0.0	1.2211652358680653E-4	4.884660943472261E-4	2.4423304717361306E-4
19	0.0	0.0	1.2211652358680653E-4	4.884660943472261E-4	2.4423304717361306E-4
20	0.0	0.0	1.2211652358680653E-4	4.884660943472261E-4	3.663495707604196E-4
21	0.0	0.0	1.2211652358680653E-4	0.0010990487122812588	3.663495707604196E-4
22	0.0	0.0	1.2211652358680653E-4	0.0012211652358680653	3.663495707604196E-4
23	0.0	0.0	1.2211652358680653E-4	0.003052913089670163	3.663495707604196E-4
24	0.0	0.0	1.2211652358680653E-4	0.004518311372711842	3.663495707604196E-4
25	0.0	0.0	1.2211652358680653E-4	0.00598370965575352	3.663495707604196E-4
26	0.0	0.0	1.2211652358680653E-4	0.007693340985968812	3.663495707604196E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTATA	40	1.5922626E-4	24.001652	2
TGTATAC	35	0.002066678	22.85592	3
GTACTAT	45	3.515011E-4	21.351755	1
GTGCTAT	40	0.0044600596	20.017271	1
TAGCGGC	265	0.0	18.715982	30
CTAGCGG	265	0.0	18.715982	29
TCTAGCG	265	0.0	18.714838	28
GGACCGT	60	1.13974114E-4	18.662249	6
GTATAGA	80	2.7712485E-6	18.015543	1
GTAAGTC	45	0.008850885	17.776829	3
TATCCTT	55	0.0013669158	17.44935	5
GTCCTAT	230	0.0	17.406322	1
GCCTAAT	65	2.08567E-4	17.24565	1
ATCGTTT	245	0.0	16.978683	29
CTATTCC	260	0.0	16.61044	4
AGCGGCG	320	0.0	16.499119	31
ACCGTCG	235	0.0	16.339554	8
CATCGTT	255	0.0	16.311857	28
GTAATAA	60	0.002427077	16.013815	1
CCTAATA	60	0.0024399813	16.001099	2
>>END_MODULE