FastQCFastQC Report
Thu 2 Feb 2017
SRR4062994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818890
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35080.4283847647425173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24750.3022383958773462No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16710.2040567109135537No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15930.1945316220737828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15110.18451806713966468No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14230.1737718130640257No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13940.1702304338800083No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13640.16656693817240412No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13110.16009476242230336No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12760.15582068409676514No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11880.14507443002112616No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11570.14128881778993516No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11260.13750320555874415No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11230.13713685598798372No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10960.13383970985113997No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10720.1309089132850566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10570.12907716543125453No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10410.1271233010538656No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9520.11625493045463982No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9330.11393471650649048No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9250.11295778431779605No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG9060.11063757036964672No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8910.10880582251584461No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG8410.10269999633650428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC601.763201E-723.9983981
ATTACAC953.6379788E-1223.5788123
TATTACA1151.8189894E-1220.8694462
CCTAATA400.00448254619.9998862
GTTATAT801.2652708E-719.9986651
GGACCGT450.00883600817.7820196
GTAAGAC450.00884845217.7776763
TCCCATA450.00884845217.7776762
TAAGCTC450.008851564517.7765912
CTAGCGG2550.017.56745329
GATATTG550.001364243917.4544452
ATATTGT652.0995656E-417.230673
CGTCTAT652.1005457E-417.229621
GTGATAA753.23371E-517.0655271
TCTAGCG2650.016.90453128
TTACACA1304.793037E-915.9999084
ATACACT1102.0172229E-715.9999084
GTATAAT805.6209028E-515.9989321
CGATGTT600.002442189315.99893220
GAGTAAT703.6966562E-415.9989311
Produced by FastQC (version 0.11.5)