##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062994_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	818890
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17718008523733	32.0	32.0	32.0	32.0	32.0
2	31.31417162256225	32.0	32.0	32.0	32.0	32.0
3	31.38072512791706	32.0	32.0	32.0	32.0	32.0
4	31.48461698152377	32.0	32.0	32.0	32.0	32.0
5	31.401000134328175	32.0	32.0	32.0	32.0	32.0
6	34.92310566742786	36.0	36.0	36.0	36.0	36.0
7	34.94755828011088	36.0	36.0	36.0	36.0	36.0
8	34.889734885027295	36.0	36.0	36.0	32.0	36.0
9	34.98222716115718	36.0	36.0	36.0	36.0	36.0
10	34.83434771458926	36.0	36.0	36.0	32.0	36.0
11	35.00214070265848	36.0	36.0	36.0	36.0	36.0
12	34.91149116486952	36.0	36.0	36.0	32.0	36.0
13	34.95580725127917	36.0	36.0	36.0	36.0	36.0
14	34.90070705467157	36.0	36.0	36.0	32.0	36.0
15	34.874613195911536	36.0	36.0	36.0	32.0	36.0
16	34.870265847671845	36.0	36.0	36.0	32.0	36.0
17	34.83451989888752	36.0	36.0	36.0	32.0	36.0
18	34.83750320555875	36.0	36.0	36.0	32.0	36.0
19	34.82741149604953	36.0	36.0	36.0	32.0	36.0
20	34.80643920428873	36.0	36.0	36.0	32.0	36.0
21	34.79283420239592	36.0	36.0	36.0	32.0	36.0
22	34.777220383690114	36.0	36.0	36.0	32.0	36.0
23	34.71478586867589	36.0	36.0	36.0	32.0	36.0
24	34.6797017914494	36.0	36.0	36.0	32.0	36.0
25	34.670249972523784	36.0	36.0	36.0	32.0	36.0
26	34.5851457460709	36.0	36.0	36.0	32.0	36.0
27	34.5784794050483	36.0	36.0	36.0	32.0	36.0
28	34.53581433403754	36.0	36.0	36.0	32.0	36.0
29	34.49021968762593	36.0	36.0	36.0	32.0	36.0
30	34.47474386059178	36.0	36.0	36.0	32.0	36.0
31	34.45975894198244	36.0	36.0	36.0	32.0	36.0
32	34.43496318186814	36.0	36.0	36.0	32.0	36.0
33	34.39246418932946	36.0	36.0	36.0	32.0	36.0
34	34.37070790948723	36.0	36.0	36.0	32.0	36.0
35	34.33112505953181	36.0	36.0	36.0	32.0	36.0
36	34.28852959493949	36.0	36.0	36.0	32.0	36.0
37	34.28089853338055	36.0	36.0	36.0	32.0	36.0
38	33.84406941103201	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	10.0
21	17.0
22	77.0
23	212.0
24	598.0
25	1506.0
26	3173.0
27	6044.0
28	10352.0
29	16074.0
30	24084.0
31	34126.0
32	48293.0
33	75533.0
34	181802.0
35	416986.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.438213307376934	18.272026809671118	11.909004419834174	26.380755463117772
2	15.952569942238885	20.878628387207073	36.03267838171183	27.136123288842214
3	18.012431462101137	24.8121237284617	28.44850956782962	28.726935241607542
4	12.166225011906361	15.951715126573776	35.43858149446202	36.44347836705785
5	14.37995335148799	36.615174199220895	32.7388293910049	16.26604305828622
6	34.35646877228632	35.549428004806515	16.419583630484265	13.674519592422897
7	30.166115309889374	30.562506029510715	20.759101660908865	18.512276999691043
8	28.632907095029854	31.923421206566616	19.411163446167823	20.032508252235708
9	27.838960696386177	14.003805308869936	18.274031087272977	39.88320290747091
10	16.37771756054254	25.945704352370964	30.635728739130062	27.040849347956435
11	38.04648220494327	21.021528947268795	21.64594047024428	19.286048377543654
12	25.38616439142397	23.796567780414147	27.677445117580323	23.139822710581555
13	29.756889237329737	18.85244099911343	25.060997501483723	26.32967226207311
14	24.269560014165517	19.759918914628336	24.43991256456911	31.53060850663703
15	25.686355920819647	26.336870641966563	22.40484069899498	25.571932738218813
16	26.40818669174126	25.15038649879715	23.21703769737083	25.224389112090755
17	24.382395681959725	25.43943631012712	24.955854876723368	25.222313131189782
18	25.797359840760052	23.51732222887079	26.327589786173967	24.35772814419519
19	26.072610484924713	24.59145917034034	25.2693279927707	24.066602351964246
20	26.139896689421043	23.18455470209674	25.320983282247923	25.35456532623429
21	27.747072256347003	23.54321093187119	23.95816287901916	24.751553932762643
22	26.22028138123726	23.680865172202825	24.703592306161152	25.395261140398762
23	24.289494668996653	23.16553281336054	25.657445636710225	26.887526880932583
24	24.96232705247347	24.82262574949016	25.00641111748831	25.208636080548057
25	25.330783537207118	23.558748499491383	25.163850045610577	25.946617917690922
26	24.905787102052777	24.728596026328322	25.653506575974795	24.712110295644106
27	25.981176936068255	23.996414654489193	24.697120122507446	25.325288286935105
28	24.746547155295584	23.650185006533235	25.967223925069298	25.63604391310188
29	24.523928732796836	24.281893783047785	25.89920502143145	25.29497246272393
30	24.926058444968188	24.29227368755266	26.054537239433866	24.72713062804528
31	25.353222044474837	24.188474642503877	24.4622598883855	25.99604342463579
32	24.936926815567414	24.345638608360098	24.520143120565642	26.197291455506843
33	24.42501434869152	23.73517810694965	25.515759137368875	26.32404840698995
34	25.41660052021639	23.941799264858528	25.738988142485557	24.902612072439524
35	26.540927352880118	23.620266458254466	25.427224657768445	24.411581531096974
36	24.900536091538548	24.70734775122422	24.780861898423474	25.61125425881376
37	26.09740013921284	24.56434930210407	24.80333133876345	24.534919219919647
38	24.971516286090058	24.127812194326705	25.30208611911993	25.598585400463307
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	313.0
1	234.0
2	155.0
3	155.0
4	496.5
5	838.0
6	838.0
7	1009.5
8	1181.0
9	1102.5
10	1024.0
11	1024.0
12	1340.5
13	1657.0
14	1995.5
15	2334.0
16	2334.0
17	3512.5
18	4691.0
19	4691.0
20	5183.0
21	5675.0
22	5601.0
23	5527.0
24	5527.0
25	5905.5
26	6284.0
27	6284.0
28	8256.5
29	10229.0
30	12893.0
31	15557.0
32	15557.0
33	20692.5
34	25828.0
35	25828.0
36	28360.0
37	30892.0
38	35665.0
39	40438.0
40	40438.0
41	43493.5
42	46549.0
43	53828.0
44	61107.0
45	61107.0
46	65429.0
47	69751.0
48	69751.0
49	73527.0
50	77303.0
51	76705.0
52	76107.0
53	76107.0
54	72902.5
55	69698.0
56	69698.0
57	69113.5
58	68529.0
59	61783.0
60	55037.0
61	55037.0
62	53114.0
63	51191.0
64	42208.5
65	33226.0
66	33226.0
67	28004.5
68	22783.0
69	22783.0
70	18341.5
71	13900.0
72	10884.0
73	7868.0
74	7868.0
75	5854.5
76	3841.0
77	3841.0
78	3884.5
79	3928.0
80	3045.0
81	2162.0
82	2162.0
83	2183.0
84	2204.0
85	2204.0
86	1383.0
87	562.0
88	492.5
89	423.0
90	423.0
91	242.0
92	61.0
93	42.0
94	23.0
95	23.0
96	15.5
97	8.0
98	8.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00989143841053133
2	0.0
3	0.0
4	0.0
5	0.0
6	2.4423304717361306E-4
7	1.2211652358680653E-4
8	0.002564446995322937
9	0.004151961801951422
10	3.663495707604196E-4
11	0.004762544419885454
12	3.663495707604196E-4
13	4.884660943472261E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.2211652358680653E-4
23	1.2211652358680653E-4
24	0.0
25	1.2211652358680653E-4
26	0.0
27	1.2211652358680653E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.2211652358680653E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	818890.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.47667585103739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.84108289025241	41.09953543537331
2	12.623297860218402	12.99610824243113
3	3.5018077477383294	5.40784266968932
4	1.3505686985974998	2.780911484490437
5	0.706437269183596	1.8182521157428009
6	0.4116160268018601	1.2713174872062765
7	0.2732519029476234	0.9846269743599758
8	0.17879710143814992	0.7363104347069357
9	0.13550447469558158	0.6277787928242596
>10	0.7309126642305583	7.315773871870117
>50	0.10584098074145756	3.89482468713978
>100	0.12395742680311192	13.564110069664748
>500	0.012634122346835638	4.264783562790596
>1k	0.004290834004585688	3.237824171710334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3508	0.4283847647425173	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2475	0.3022383958773462	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1671	0.2040567109135537	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1593	0.1945316220737828	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1511	0.18451806713966468	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1423	0.1737718130640257	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1394	0.1702304338800083	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1364	0.16656693817240412	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1311	0.16009476242230336	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1276	0.15582068409676514	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1188	0.14507443002112616	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1157	0.14128881778993516	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1126	0.13750320555874415	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1123	0.13713685598798372	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1096	0.13383970985113997	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1072	0.1309089132850566	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1057	0.12907716543125453	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1041	0.1271233010538656	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	952	0.11625493045463982	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	933	0.11393471650649048	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	925	0.11295778431779605	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	906	0.11063757036964672	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	891	0.10880582251584461	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	841	0.10269999633650428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2211652358680653E-4
2	0.0	0.0	0.0	0.0	1.2211652358680653E-4
3	0.0	0.0	0.0	0.0	1.2211652358680653E-4
4	0.0	0.0	0.0	0.0	1.2211652358680653E-4
5	0.0	0.0	0.0	1.2211652358680653E-4	1.2211652358680653E-4
6	0.0	0.0	0.0	1.2211652358680653E-4	1.2211652358680653E-4
7	0.0	0.0	0.0	1.2211652358680653E-4	1.2211652358680653E-4
8	0.0	0.0	0.0	1.2211652358680653E-4	1.2211652358680653E-4
9	0.0	0.0	0.0	1.2211652358680653E-4	1.2211652358680653E-4
10	0.0	0.0	0.0	1.2211652358680653E-4	1.2211652358680653E-4
11	0.0	0.0	0.0	1.2211652358680653E-4	1.2211652358680653E-4
12	0.0	0.0	0.0	1.2211652358680653E-4	2.4423304717361306E-4
13	0.0	0.0	0.0	1.2211652358680653E-4	2.4423304717361306E-4
14	0.0	0.0	0.0	1.2211652358680653E-4	2.4423304717361306E-4
15	0.0	0.0	0.0	1.2211652358680653E-4	2.4423304717361306E-4
16	0.0	0.0	0.0	2.4423304717361306E-4	3.663495707604196E-4
17	0.0	0.0	0.0	2.4423304717361306E-4	3.663495707604196E-4
18	0.0	0.0	0.0	3.663495707604196E-4	4.884660943472261E-4
19	0.0	0.0	0.0	3.663495707604196E-4	4.884660943472261E-4
20	0.0	0.0	0.0	3.663495707604196E-4	6.105826179340326E-4
21	0.0	0.0	0.0	9.769321886944522E-4	6.105826179340326E-4
22	0.0	0.0	0.0	0.0010990487122812588	6.105826179340326E-4
23	0.0	0.0	0.0	0.0029307965660833567	6.105826179340326E-4
24	0.0	0.0	0.0	0.004396194849125035	6.105826179340326E-4
25	0.0	0.0	0.0	0.005861593132166713	6.105826179340326E-4
26	0.0	0.0	0.0	0.007571224462382005	6.105826179340326E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	60	1.763201E-7	23.998398	1
ATTACAC	95	3.6379788E-12	23.578812	3
TATTACA	115	1.8189894E-12	20.869446	2
CCTAATA	40	0.004482546	19.999886	2
GTTATAT	80	1.2652708E-7	19.998665	1
GGACCGT	45	0.008836008	17.782019	6
GTAAGAC	45	0.008848452	17.777676	3
TCCCATA	45	0.008848452	17.777676	2
TAAGCTC	45	0.0088515645	17.77659	12
CTAGCGG	255	0.0	17.567453	29
GATATTG	55	0.0013642439	17.454445	2
ATATTGT	65	2.0995656E-4	17.23067	3
CGTCTAT	65	2.1005457E-4	17.22962	1
GTGATAA	75	3.23371E-5	17.065527	1
TCTAGCG	265	0.0	16.904531	28
TTACACA	130	4.793037E-9	15.999908	4
ATACACT	110	2.0172229E-7	15.999908	4
GTATAAT	80	5.6209028E-5	15.998932	1
CGATGTT	60	0.0024421893	15.998932	20
GAGTAAT	70	3.6966562E-4	15.998931	1
>>END_MODULE