FastQCFastQC Report
Thu 2 Feb 2017
SRR4062993_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062993_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016194
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT43920.21783618044692127No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC41130.20399822636115372No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT34650.17185846203291943No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA34060.1689321563301944No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC32040.15891327917849177No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG31820.1578221143401875No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC30910.15330865978174718No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG30740.15246548695214845No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC29200.1448273330840187No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC28800.14284339701437462No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG28580.14175223217607036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27740.13758596642981777No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG27320.13550283355669146No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG27100.13441166871838722No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG26650.13217974064003762No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC25900.12845986050945493No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA24980.12389680754927354No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA24960.12379761074579132No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG23800.11804419614382346No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC23580.1169530313055192No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT23270.11541548085154504No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG22910.11362993838886536No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC22550.11184439592618568No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC20670.10251989639885845No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT20660.10247029799711733No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG20340.10088314914140208No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT20200.10018877151702664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24850.019.2644121
TCTAGCG7600.018.52585628
GGTATCA10500.018.297971
CTAGCGG7700.018.28571529
CAAGACG10550.017.5889264
AAGACGG10300.017.5490465
GTTATAT1404.0017767E-1117.1543481
TAGCGGC8500.016.75294130
TACCGTC6700.016.4763857
ACCGTCG6850.016.3491488
GCGCAAG10650.016.0858921
GACGGAC11000.015.998817
CGCAAGA10750.015.9267672
CGGTCCA9450.015.91613410
ACGAACG3550.015.77582115
ATCGTTT6750.015.64444429
CGAACGA3700.015.56833916
CGCCGGT9150.015.5616837
GCCGGTC9600.015.4988478
ACCCGTT3000.015.46666530
Produced by FastQC (version 0.11.5)