##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062993_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2016194
Sequences flagged as poor quality	0
Sequence length	38
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.331864889985784	32.0	32.0	32.0	32.0	32.0
2	30.99840987524018	32.0	32.0	32.0	32.0	32.0
3	31.04110021158678	32.0	32.0	32.0	32.0	32.0
4	31.10241970762734	32.0	32.0	32.0	32.0	32.0
5	31.018178806206148	32.0	32.0	32.0	32.0	32.0
6	34.66246750064726	36.0	36.0	36.0	32.0	36.0
7	34.625127839880484	36.0	36.0	36.0	32.0	36.0
8	34.61332242829807	36.0	36.0	36.0	32.0	36.0
9	34.74541189984694	36.0	36.0	36.0	32.0	36.0
10	34.48306561769353	36.0	36.0	36.0	32.0	36.0
11	34.69396893354509	36.0	36.0	36.0	32.0	36.0
12	34.53759806843984	36.0	36.0	36.0	32.0	36.0
13	34.62717278198427	36.0	36.0	36.0	32.0	36.0
14	34.52554268091265	36.0	36.0	36.0	32.0	36.0
15	34.46374009643913	36.0	36.0	36.0	32.0	36.0
16	34.483476292459954	36.0	36.0	36.0	32.0	36.0
17	34.39772809560985	36.0	36.0	36.0	32.0	36.0
18	34.43322864764006	36.0	36.0	36.0	32.0	36.0
19	34.416181180977624	36.0	36.0	36.0	32.0	36.0
20	34.40746723777573	36.0	36.0	36.0	32.0	36.0
21	34.393865868066264	36.0	36.0	36.0	32.0	36.0
22	34.37318085462014	36.0	36.0	36.0	32.0	36.0
23	34.31399160993436	36.0	36.0	36.0	32.0	36.0
24	34.29966263167136	36.0	36.0	36.0	32.0	36.0
25	34.27934613435016	36.0	36.0	36.0	32.0	36.0
26	34.221716263415125	36.0	36.0	36.0	32.0	36.0
27	34.21875771875127	36.0	36.0	36.0	32.0	36.0
28	34.212557422549615	36.0	36.0	36.0	32.0	36.0
29	34.19189373641624	36.0	36.0	36.0	32.0	36.0
30	34.15301156535532	36.0	36.0	36.0	32.0	36.0
31	34.187091619159666	36.0	36.0	36.0	32.0	36.0
32	34.14378725459951	36.0	36.0	36.0	32.0	36.0
33	34.10169953883406	36.0	36.0	36.0	32.0	36.0
34	34.12374801234405	36.0	36.0	36.0	32.0	36.0
35	34.08395223872306	36.0	36.0	36.0	32.0	36.0
36	34.04906025908221	36.0	36.0	36.0	32.0	36.0
37	34.058473539748654	36.0	36.0	36.0	32.0	36.0
38	33.44119514292771	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	6.0
9	5.0
10	22.0
11	22.0
12	14.0
13	15.0
14	127.0
15	349.0
16	531.0
17	590.0
18	791.0
19	1021.0
20	1442.0
21	2043.0
22	3168.0
23	4572.0
24	7180.0
25	10669.0
26	16248.0
27	23851.0
28	33908.0
29	47060.0
30	64663.0
31	88793.0
32	124824.0
33	194436.0
34	446663.0
35	943180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.99824412955304	16.67684634544566	11.078618900634417	26.246290624366875
2	17.780911868100894	18.738673108644864	35.94959297628524	27.530822046969007
3	19.56330547733267	22.180168981275948	27.632798816248116	30.623726725143268
4	13.078687687084445	14.305242456482329	34.605519967660896	38.010549888772324
5	15.708954594647142	35.38345020370063	32.67691501909042	16.230680182561798
6	36.225084726932025	34.083116463138516	15.879014950527804	13.81278385940165
7	32.10496217376015	29.25979804512762	19.740768864885457	18.89447091622677
8	29.445077376101768	31.600279933795957	18.697087726311644	20.257554963790632
9	27.411255883622076	14.018123291951651	18.10533729460021	40.46528352982606
10	16.885842873623762	25.118466252617566	29.74735465874676	28.24833621501191
11	39.31439523010971	20.426833956055784	20.98517618010988	19.273594633724624
12	25.729008411664132	22.86108676577739	26.959634031354145	24.450270791204332
13	29.58026437976713	17.94286190502647	25.037849324962497	27.439024390243905
14	24.216450023189655	19.070330384402663	23.4086563144071	33.30456327800058
15	25.97678933140713	26.236499180686117	21.13298979863681	26.653721689269936
16	27.333920979308544	24.79541933765321	22.33916167688433	25.531498006153914
17	25.275267702336336	25.22342634186029	23.703606323157707	25.79769963264567
18	26.653740502543073	23.451365183481094	24.7527610703221	25.14213324365373
19	26.457946952362484	24.03988617272401	24.182691091482944	25.319475783430562
20	26.61846059679564	23.203246717495006	23.584781656562008	26.593511029147344
21	28.209186649577195	23.17400905530402	23.237697762363744	25.379106532755042
22	27.078534311887285	22.964123656522005	23.9834010900599	25.973940941530806
23	25.116220929886484	22.861594414252465	24.833828375962554	27.18835627989849
24	25.649681136875024	24.126549174765564	24.067816452305458	26.155953236053954
25	25.845726004319786	23.516128744217056	24.073563305060613	26.564581946402544
26	25.58159031932093	24.732005859682143	24.56249575232574	25.12390806867119
27	26.581756078735996	23.444012732123912	23.724518267085912	26.249712922054186
28	25.477364850788764	23.517807931081688	24.877484291204464	26.127342926925085
29	25.104329584760066	23.990918502138182	24.879226693690917	26.025525219410834
30	25.027503241364872	24.271151703812162	25.033752850764284	25.667592204058675
31	26.172730627599122	24.05171661217104	23.397074696041393	26.37847806418844
32	25.92988124721917	23.580271592237644	23.321694988732848	27.168152171810338
33	25.164198664386912	23.358248970859666	24.35313836338537	27.124414001368052
34	26.57115064186352	23.59579488741408	24.209309166452954	25.623745304269452
35	27.089403937372698	23.19156767675565	24.58908397499432	25.129944410877332
36	25.162311570751378	24.331109651585713	23.931581109155523	26.57499766850739
37	26.897260199227574	23.82218924139354	23.868469324687773	25.412081234691115
38	25.43148431795816	23.37981333925907	24.553784006856922	26.634918335925846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	50.5
2	77.0
3	77.0
4	240.0
5	403.0
6	403.0
7	510.0
8	617.0
9	605.5
10	594.0
11	594.0
12	802.0
13	1010.0
14	1482.0
15	1954.0
16	1954.0
17	3069.5
18	4185.0
19	4185.0
20	5389.0
21	6593.0
22	7407.0
23	8221.0
24	8221.0
25	9936.5
26	11652.0
27	11652.0
28	15120.5
29	18589.0
30	24001.5
31	29414.0
32	29414.0
33	41397.0
34	53380.0
35	53380.0
36	61449.5
37	69519.0
38	82247.5
39	94976.0
40	94976.0
41	102950.0
42	110924.0
43	130376.5
44	149829.0
45	149829.0
46	153310.0
47	156791.0
48	156791.0
49	172198.0
50	187605.0
51	195721.5
52	203838.0
53	203838.0
54	195523.0
55	187208.0
56	187208.0
57	183634.0
58	180060.0
59	162469.5
60	144879.0
61	144879.0
62	140146.5
63	135414.0
64	114037.0
65	92660.0
66	92660.0
67	78333.5
68	64007.0
69	64007.0
70	51687.0
71	39367.0
72	30578.5
73	21790.0
74	21790.0
75	16592.0
76	11394.0
77	11394.0
78	11001.0
79	10608.0
80	8388.0
81	6168.0
82	6168.0
83	6307.5
84	6447.0
85	6447.0
86	4261.5
87	2076.0
88	1781.0
89	1486.0
90	1486.0
91	1056.0
92	626.0
93	542.0
94	458.0
95	458.0
96	412.0
97	366.0
98	366.0
99	675.5
100	985.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11794499934034126
2	0.05346707707690828
3	0.01145723080219463
4	0.0029263057027250354
5	0.0
6	5.455824191521253E-4
7	4.95984017411023E-5
8	1.487952052233069E-4
9	1.983936069644092E-4
10	3.967872139288184E-4
11	0.002331124881831808
12	0.003223896113171649
13	0.015177110932777302
14	0.01036606596389038
15	0.02395602804095241
16	0.011953214819605653
17	0.021277714346932885
18	0.0063485954228610945
19	0.00778694907335306
20	0.006298997021119992
21	0.0068445794402721164
22	0.007836547475094163
23	0.010217270758667072
24	0.013986749290990847
25	0.01745863741286801
26	0.019095384670324383
27	0.009473294732550538
28	0.006249398619378889
29	0.011258837195230222
30	0.0033726913183949564
31	0.006447792226343298
32	0.00778694907335306
33	0.009473294732550538
34	0.013242773264874311
35	0.016466669378045962
36	0.01577229175367053
37	0.010316467562149278
38	0.006447792226343298
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2016194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.62375103020205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29002404382854	39.04002139316056
2	11.34371309218578	11.031477623049698
3	3.4627216325189107	5.05111543639483
4	1.5098064032448009	2.9364980262072016
5	0.8614717345796725	2.094399357087915
6	0.521647907987156	1.5218686802035932
7	0.35446246390966896	1.2064706216287004
8	0.2550329603094678	0.9920527333266373
9	0.20293553801428713	0.888073836802812
>10	1.0098409309286964	8.748739469634067
>50	0.0750914531247413	2.5446592153108165
>100	0.08458040000158447	8.921582730396867
>500	0.01798387773894521	6.070187554894338
>1k	0.010687561627716011	8.952853321901971
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	4392	0.21783618044692127	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4113	0.20399822636115372	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3465	0.17185846203291943	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3406	0.1689321563301944	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3204	0.15891327917849177	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3182	0.1578221143401875	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3091	0.15330865978174718	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3074	0.15246548695214845	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2920	0.1448273330840187	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2880	0.14284339701437462	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2858	0.14175223217607036	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2774	0.13758596642981777	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2732	0.13550283355669146	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2710	0.13441166871838722	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2665	0.13217974064003762	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2590	0.12845986050945493	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2498	0.12389680754927354	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2496	0.12379761074579132	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2380	0.11804419614382346	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2358	0.1169530313055192	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2327	0.11541548085154504	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2291	0.11362993838886536	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	2255	0.11184439592618568	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	2067	0.10251989639885845	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2066	0.10247029799711733	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	2034	0.10088314914140208	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2020	0.10018877151702664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.9598401741102294E-5
2	0.0	0.0	0.0	0.0	4.9598401741102294E-5
3	0.0	0.0	0.0	0.0	4.9598401741102294E-5
4	0.0	0.0	0.0	0.0	4.9598401741102294E-5
5	0.0	0.0	0.0	0.0	4.9598401741102294E-5
6	0.0	0.0	0.0	0.0	4.9598401741102294E-5
7	0.0	0.0	0.0	9.919680348220459E-5	4.9598401741102294E-5
8	0.0	0.0	0.0	9.919680348220459E-5	1.487952052233069E-4
9	0.0	0.0	0.0	1.9839360696440918E-4	1.487952052233069E-4
10	0.0	0.0	0.0	1.9839360696440918E-4	1.487952052233069E-4
11	0.0	0.0	0.0	1.9839360696440918E-4	1.487952052233069E-4
12	0.0	0.0	0.0	1.9839360696440918E-4	4.4638561566992063E-4
13	0.0	0.0	0.0	1.9839360696440918E-4	4.4638561566992063E-4
14	0.0	0.0	0.0	1.9839360696440918E-4	4.4638561566992063E-4
15	0.0	0.0	0.0	2.4799200870551146E-4	4.4638561566992063E-4
16	0.0	0.0	0.0	2.4799200870551146E-4	4.4638561566992063E-4
17	0.0	0.0	0.0	2.4799200870551146E-4	4.4638561566992063E-4
18	0.0	0.0	0.0	2.975904104466138E-4	4.4638561566992063E-4
19	0.0	0.0	0.0	2.975904104466138E-4	4.4638561566992063E-4
20	0.0	0.0	0.0	2.975904104466138E-4	5.455824191521252E-4
21	0.0	0.0	0.0	3.4718881218771607E-4	6.447792226343299E-4
22	0.0	0.0	0.0	5.455824191521252E-4	6.447792226343299E-4
23	0.0	0.0	0.0	5.951808208932276E-4	6.447792226343299E-4
24	0.0	0.0	0.0	6.943776243754321E-4	6.447792226343299E-4
25	0.0	0.0	0.0	7.935744278576367E-4	6.447792226343299E-4
26	0.0	0.0	0.0	0.0011407632400453529	6.943776243754321E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2485	0.0	19.264412	1
TCTAGCG	760	0.0	18.525856	28
GGTATCA	1050	0.0	18.29797	1
CTAGCGG	770	0.0	18.285715	29
CAAGACG	1055	0.0	17.588926	4
AAGACGG	1030	0.0	17.549046	5
GTTATAT	140	4.0017767E-11	17.154348	1
TAGCGGC	850	0.0	16.752941	30
TACCGTC	670	0.0	16.476385	7
ACCGTCG	685	0.0	16.349148	8
GCGCAAG	1065	0.0	16.085892	1
GACGGAC	1100	0.0	15.99881	7
CGCAAGA	1075	0.0	15.926767	2
CGGTCCA	945	0.0	15.916134	10
ACGAACG	355	0.0	15.775821	15
ATCGTTT	675	0.0	15.644444	29
CGAACGA	370	0.0	15.568339	16
CGCCGGT	915	0.0	15.561683	7
GCCGGTC	960	0.0	15.498847	8
ACCCGTT	300	0.0	15.466665	30
>>END_MODULE