FastQCFastQC Report
Thu 2 Feb 2017
SRR4062993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016194
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC44690.22165525738098618No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT43240.21446348912852634No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG33960.1684361723127834No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC33770.16749380267970243No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT33660.1669482202605503No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC33400.16565866181528166No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA33330.16531147300309396No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC32360.16050042803420703No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG31100.15425102941482816No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG29990.1487456068215658No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG29530.1464640803414751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28250.14011548491861398No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC26600.13193174863133209No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG26060.1292534349373126No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG26040.12915423813383037No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC25180.12488877558409557No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA24950.12374801234405022No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC24480.12141688746221842No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24440.121218493855254No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG23490.1165066456898493No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA23260.11536588244980395No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT22870.11343154478190094No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA22750.11283636396100773No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG22310.11065403428439921No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT21550.10688455575207545No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG21010.10420624205805591No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT20910.1037102580406449No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG20860.10346226603193938No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC20650.10242069959537624No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT20210.10023836991876772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25250.020.5954951
CTAGCGG8200.020.09696829
TCTAGCG8300.020.04760428
ACGATTA659.442007E-619.69172732
TAGCGGC8750.018.8337330
TACCGTC7550.018.2268897
ACCGTCG7550.018.0145028
CGTCGTA7800.017.02598410
GTCCTAG1601.8189894E-1217.0011861
CGCGGTC4000.016.80033910
ATACCGT8150.016.6886946
CCGTCGT8000.016.6007489
GTCGTAG8300.016.57864811
GGTATCA9000.016.3566951
AGCGGCG10400.016.1533731
AGAACCG4800.015.6681485
TCAGATA8750.015.360312
AGATACC8700.015.2650554
CGGTCCA11250.015.21808410
GGTCCTA4350.015.08001513
Produced by FastQC (version 0.11.5)