##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062993_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2016194
Sequences flagged as poor quality	0
Sequence length	38
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.216174633988594	32.0	32.0	32.0	32.0	32.0
2	31.229688214526973	32.0	32.0	32.0	32.0	32.0
3	31.328188160464716	32.0	32.0	32.0	32.0	32.0
4	31.441070154955327	32.0	32.0	32.0	32.0	32.0
5	31.33534868172408	32.0	32.0	32.0	32.0	32.0
6	34.87832867273685	36.0	36.0	36.0	36.0	36.0
7	34.90297015068987	36.0	36.0	36.0	36.0	36.0
8	34.832674831886216	36.0	36.0	36.0	32.0	36.0
9	34.96366669080456	36.0	36.0	36.0	32.0	36.0
10	34.78885117205983	36.0	36.0	36.0	32.0	36.0
11	34.97232111592436	36.0	36.0	36.0	36.0	36.0
12	34.8577398801901	36.0	36.0	36.0	32.0	36.0
13	34.93026811904013	36.0	36.0	36.0	32.0	36.0
14	34.87054569153564	36.0	36.0	36.0	32.0	36.0
15	34.834440039004186	36.0	36.0	36.0	32.0	36.0
16	34.84503971344027	36.0	36.0	36.0	32.0	36.0
17	34.80409672878701	36.0	36.0	36.0	32.0	36.0
18	34.80897026774209	36.0	36.0	36.0	32.0	36.0
19	34.80221942928111	36.0	36.0	36.0	32.0	36.0
20	34.79455945211622	36.0	36.0	36.0	32.0	36.0
21	34.7900638529824	36.0	36.0	36.0	32.0	36.0
22	34.77310070360293	36.0	36.0	36.0	32.0	36.0
23	34.70765858840965	36.0	36.0	36.0	32.0	36.0
24	34.678208545407834	36.0	36.0	36.0	32.0	36.0
25	34.65552769227564	36.0	36.0	36.0	32.0	36.0
26	34.587650791540895	36.0	36.0	36.0	32.0	36.0
27	34.57474379945581	36.0	36.0	36.0	32.0	36.0
28	34.54953094791473	36.0	36.0	36.0	32.0	36.0
29	34.50928234088585	36.0	36.0	36.0	32.0	36.0
30	34.487337528035496	36.0	36.0	36.0	32.0	36.0
31	34.490799000493006	36.0	36.0	36.0	32.0	36.0
32	34.46581182167986	36.0	36.0	36.0	32.0	36.0
33	34.43552257372058	36.0	36.0	36.0	32.0	36.0
34	34.43536286686698	36.0	36.0	36.0	32.0	36.0
35	34.407503444609	36.0	36.0	36.0	32.0	36.0
36	34.37486521634327	36.0	36.0	36.0	32.0	36.0
37	34.38788330884825	36.0	36.0	36.0	32.0	36.0
38	33.90785212137324	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	11.0
21	39.0
22	129.0
23	443.0
24	1353.0
25	3350.0
26	6955.0
27	13550.0
28	23907.0
29	37831.0
30	57583.0
31	84033.0
32	122089.0
33	199601.0
34	480910.0
35	984409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.263710524671055	17.998359131867662	11.794706960197539	26.943223383263742
2	16.684960219582155	19.70133811594426	35.2904707039455	28.323230960528083
3	18.446439181943802	23.012864833443608	27.901878489867542	30.63881749474505
4	12.482119722962343	15.25879531075475	34.73202895761705	37.52705600866586
5	15.107177186322348	35.98130933828788	32.29406495605085	16.61744851933891
6	36.18617717503386	34.59981638607927	15.50798611438324	13.706020324503628
7	31.844426357408107	29.465964545771527	19.772767966304546	18.916841130515824
8	29.33312303089462	31.8504035426041	18.43781991762493	20.378653508876347
9	27.396936171479165	13.840449778161357	17.876797091421327	40.88581695893815
10	16.619316350044492	25.362145604207566	29.726393205361827	28.29214484038612
11	39.473000240068416	20.393960196260885	20.823801691589473	19.309237872081233
12	25.51871428592685	23.026633938221007	26.8385323385182	24.61611943733394
13	29.571737215503287	17.974081833476177	25.035562089324827	27.41861886169571
14	24.169598758849595	19.00209007664937	23.355589789474624	33.47272137502641
15	26.004194040851225	26.355003536366045	20.988555664782258	26.65224675800047
16	27.318317244430467	24.993589403395408	22.055031437962537	25.633061914211584
17	25.2838764523652	25.374988716363607	23.508104874828515	25.833029956442683
18	26.65631382694324	23.52764664511451	24.673468922137452	25.1425706058048
19	26.44755415401494	24.13249915434725	24.134036704801222	25.285909986836586
20	26.702837127776398	23.089246372124904	23.479238605015194	26.728677895083507
21	28.19045597321288	23.281798915084025	23.1491231246215	25.378621987081594
22	27.02557392790575	23.047534116260636	23.925723417488594	26.00116853834502
23	25.019802191359847	22.80924773496162	24.799981747761002	27.370968325917534
24	25.762451430765093	24.086422239129764	23.967683665361566	26.183442664743573
25	25.787573802495	23.45679379741612	24.17805447100276	26.57757792908612
26	25.684842671311724	24.72446834206844	24.421868342961215	25.16882064365862
27	26.720669460051422	23.352785845792464	23.614268879154366	26.312275815001744
28	25.512872273203868	23.509047244461595	24.932223784020785	26.045856698313752
29	25.349991121886088	23.818293279317366	24.800936814612086	26.03077878418446
30	25.13637080558716	24.110775054384646	25.06241958859118	25.690434551437015
31	26.234380223331684	23.943876432525837	23.44273418133374	26.379009162808735
32	26.05647075628635	23.49208459106614	23.38286891043223	27.068575742215284
33	25.238295521165128	23.279059455588104	24.42002108924042	27.062623934006353
34	26.540600755681247	23.437526349150925	24.352716058077746	25.66915683709008
35	27.141534991176446	23.061322471944663	24.585332562243515	25.21180997463538
36	25.364027469578822	24.23784615964535	23.90216417666157	26.49596219411426
37	26.951288889506113	23.881394291121932	23.761118107244695	25.40619871212726
38	25.616409548296986	23.21782846078151	24.529409897470085	26.636352093451414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	29.5
2	47.0
3	47.0
4	185.5
5	324.0
6	324.0
7	409.0
8	494.0
9	511.5
10	529.0
11	529.0
12	727.0
13	925.0
14	1481.0
15	2037.0
16	2037.0
17	3190.5
18	4344.0
19	4344.0
20	5441.0
21	6538.0
22	7429.5
23	8321.0
24	8321.0
25	9913.5
26	11506.0
27	11506.0
28	15048.5
29	18591.0
30	23970.5
31	29350.0
32	29350.0
33	41670.5
34	53991.0
35	53991.0
36	61981.5
37	69972.0
38	82664.0
39	95356.0
40	95356.0
41	103182.5
42	111009.0
43	130921.0
44	150833.0
45	150833.0
46	153427.0
47	156021.0
48	156021.0
49	171315.5
50	186610.0
51	194961.0
52	203312.0
53	203312.0
54	195483.0
55	187654.0
56	187654.0
57	184464.5
58	181275.0
59	163744.5
60	146214.0
61	146214.0
62	141174.0
63	136134.0
64	114165.0
65	92196.0
66	92196.0
67	78623.5
68	65051.0
69	65051.0
70	52255.0
71	39459.0
72	30766.0
73	22073.0
74	22073.0
75	16525.5
76	10978.0
77	10978.0
78	10762.0
79	10546.0
80	8342.0
81	6138.0
82	6138.0
83	5786.0
84	5434.0
85	5434.0
86	3518.0
87	1602.0
88	1302.0
89	1002.0
90	1002.0
91	600.0
92	198.0
93	129.0
94	60.0
95	60.0
96	41.5
97	23.0
98	23.0
99	29.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009324499527327232
2	4.95984017411023E-5
3	0.0
4	2.975904104466138E-4
5	0.0
6	3.4718881218771607E-4
7	8.431728295987391E-4
8	0.002479920087055115
9	0.004414257754958104
10	7.935744278576368E-4
11	0.005059036977592434
12	3.4718881218771607E-4
13	9.91968034822046E-5
14	0.0
15	0.0
16	4.95984017411023E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	4.95984017411023E-5
22	0.0
23	1.487952052233069E-4
24	0.0
25	9.91968034822046E-5
26	4.95984017411023E-5
27	9.91968034822046E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	4.95984017411023E-5
38	9.91968034822046E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2016194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.03620313682193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96046551006197	37.14000495509296
2	12.135506057510561	11.416162561783997
3	3.7445758713436645	5.283918940372877
4	1.6053230725754732	3.020332085675484
5	0.8676457389026828	2.040538061291228
6	0.5462531778265256	1.541620526182978
7	0.38200862027846416	1.2577764544404437
8	0.2799463496223528	1.053409069459901
9	0.2130008158503416	0.9016874678380924
>10	1.0648970485141052	8.91101973885051
>50	0.08285660439870303	2.711905471966926
>100	0.08681354597112133	8.941476101620111
>500	0.018594559689281944	5.977235860971706
>1k	0.01211302745473224	9.802912704452739
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4469	0.22165525738098618	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	4324	0.21446348912852634	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3396	0.1684361723127834	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3377	0.16749380267970243	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3366	0.1669482202605503	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3340	0.16565866181528166	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3333	0.16531147300309396	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3236	0.16050042803420703	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3110	0.15425102941482816	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2999	0.1487456068215658	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2953	0.1464640803414751	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2825	0.14011548491861398	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2660	0.13193174863133209	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2606	0.1292534349373126	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2604	0.12915423813383037	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2518	0.12488877558409557	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2495	0.12374801234405022	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2448	0.12141688746221842	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2444	0.121218493855254	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2349	0.1165066456898493	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2326	0.11536588244980395	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2287	0.11343154478190094	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2275	0.11283636396100773	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2231	0.11065403428439921	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	2155	0.10688455575207545	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	2101	0.10420624205805591	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2091	0.1037102580406449	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	2086	0.10346226603193938	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	2065	0.10242069959537624	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2021	0.10023836991876772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.9598401741102294E-5
2	0.0	0.0	0.0	0.0	4.9598401741102294E-5
3	0.0	0.0	0.0	0.0	4.9598401741102294E-5
4	0.0	0.0	0.0	4.9598401741102294E-5	4.9598401741102294E-5
5	0.0	0.0	0.0	4.9598401741102294E-5	9.919680348220459E-5
6	0.0	0.0	0.0	4.9598401741102294E-5	1.487952052233069E-4
7	0.0	0.0	0.0	4.9598401741102294E-5	1.487952052233069E-4
8	0.0	0.0	0.0	4.9598401741102294E-5	1.487952052233069E-4
9	0.0	0.0	0.0	4.9598401741102294E-5	1.487952052233069E-4
10	0.0	0.0	0.0	4.9598401741102294E-5	1.487952052233069E-4
11	0.0	0.0	0.0	4.9598401741102294E-5	1.487952052233069E-4
12	0.0	0.0	0.0	4.9598401741102294E-5	2.4799200870551146E-4
13	0.0	0.0	0.0	4.9598401741102294E-5	2.4799200870551146E-4
14	0.0	0.0	0.0	4.9598401741102294E-5	2.4799200870551146E-4
15	0.0	0.0	0.0	9.919680348220459E-5	2.975904104466138E-4
16	0.0	0.0	0.0	9.919680348220459E-5	2.975904104466138E-4
17	0.0	0.0	0.0	9.919680348220459E-5	2.975904104466138E-4
18	0.0	0.0	0.0	9.919680348220459E-5	3.4718881218771607E-4
19	0.0	0.0	0.0	9.919680348220459E-5	3.4718881218771607E-4
20	0.0	0.0	0.0	9.919680348220459E-5	3.9678721392881835E-4
21	0.0	0.0	0.0	1.487952052233069E-4	3.9678721392881835E-4
22	0.0	0.0	0.0	3.4718881218771607E-4	4.4638561566992063E-4
23	0.0	0.0	0.0	4.4638561566992063E-4	4.959840174110229E-4
24	0.0	0.0	0.0	5.455824191521252E-4	4.959840174110229E-4
25	0.0	0.0	0.0	6.447792226343299E-4	4.959840174110229E-4
26	0.0	0.0	0.0	9.919680348220458E-4	4.959840174110229E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2525	0.0	20.595495	1
CTAGCGG	820	0.0	20.096968	29
TCTAGCG	830	0.0	20.047604	28
ACGATTA	65	9.442007E-6	19.691727	32
TAGCGGC	875	0.0	18.83373	30
TACCGTC	755	0.0	18.226889	7
ACCGTCG	755	0.0	18.014502	8
CGTCGTA	780	0.0	17.025984	10
GTCCTAG	160	1.8189894E-12	17.001186	1
CGCGGTC	400	0.0	16.800339	10
ATACCGT	815	0.0	16.688694	6
CCGTCGT	800	0.0	16.600748	9
GTCGTAG	830	0.0	16.578648	11
GGTATCA	900	0.0	16.356695	1
AGCGGCG	1040	0.0	16.15337	31
AGAACCG	480	0.0	15.668148	5
TCAGATA	875	0.0	15.36031	2
AGATACC	870	0.0	15.265055	4
CGGTCCA	1125	0.0	15.218084	10
GGTCCTA	435	0.0	15.080015	13
>>END_MODULE