FastQCFastQC Report
Thu 2 Feb 2017
SRR4062992_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062992_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785152
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32140.40934748940332566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21190.2698840479295729No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20180.25702029670687965No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17050.21715540430388No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11850.1509261900880339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11830.1506714623410499No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.14608636289533747No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10710.13640670850994457No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9530.1213777714378872No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9410.11984940495598305No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9200.11717476361265079No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8510.10838665634170198No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8450.1076224731007499No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8390.10685828985979785No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8020.1021458265405934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1750.020.136251
GTATTAT801.2497549E-720.0218411
TAAACGC2000.019.19892928
TCTAGCG2200.018.90803728
GTAAACG1950.018.87074327
CTAGCGG2050.018.73066329
ACGCTTC1900.018.52528231
CGCTTCG1900.018.52528232
CGACCCG953.7955033E-818.5217445
AACGCTT1950.018.05027630
GTTATAT802.7649203E-618.0196571
GTCCTAA450.00879238617.7971921
GTACTAC450.00879238617.7971921
CTAATCC550.001366381617.450244
AAGACGG2300.017.3870125
CAAGACG2400.017.3290564
ACCGTCG1850.017.2963338
TATTAGC1404.0017767E-1117.1473642
TACCGTC1800.016.8879477
CGGCATC1651.8189894E-1216.4849825
Produced by FastQC (version 0.11.5)