FastQCFastQC Report
Thu 2 Feb 2017
SRR4062992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785152
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38320.48805836322138896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27500.3502506521030323No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21400.2725586892729051No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19400.24708591457450277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16120.20531056406912293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.17448850668405608No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11530.14685054613628953No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10770.13717089175089664No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG10380.1322037006847082No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9420.11997676882947507No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9350.11908522171503097No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9000.11462748614281056No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8690.1106792060645582No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8540.10876874796217802No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8240.10494783175741768No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8050.10252791816106945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC200.003753309831.99910724
TAGCGGC2350.021.10579530
CTAGCGG2350.021.10579529
TCTAGCG2400.020.66609228
ATGACCG400.00447965620.001995
CGGTCCA2800.020.00071510
ATCGTTT1950.019.6917629
CGCCGGT3050.017.8378417
GCCGGTC3150.017.7795478
CTTGCGC3250.017.7237113
GCGGCGC2900.017.6546832
CATCGTT2200.017.45405828
GGCATCG2200.017.45405826
CCGGTCC3400.017.4134989
CGTTTAT2050.017.17025431
TCGTTTA2150.017.11580330
CGGCATC2250.017.0661925
AGCGGCG3000.017.0661931
TCTTGCG3200.016.9995252
GCATCGC2100.016.76143814
Produced by FastQC (version 0.11.5)