FastQCFastQC Report
Thu 2 Feb 2017
SRR4062991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376412
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53580.3892729793114271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32430.23561259274112695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18730.13607844162939586No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.120312813314618No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13890.10091455174758719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG2850.018.52412828
CTAGCGG2900.018.2060729
AAACCGG450.008849747517.77826110
CGACTCA450.008849747517.77826110
TAGCGGC3200.016.99984630
ACCGTCG2450.016.976978
TACCGTC2750.016.2883937
ATCGTTT2400.015.99927229
ATAATAC1551.891749E-1015.4809183
CGTCGTA2800.015.42899210
ATACCGT2800.015.4245076
CCGTCGT2600.015.3850359
CGTTTAT2600.015.38447631
GTCTTAC1401.2656528E-814.8688941
GTATAGA1851.0913936E-1114.7143311
GTATAAT1851.0913936E-1114.7143311
GCCTTAT1103.5144985E-614.5569591
TACGCTA3150.014.22260959
TAATATA3950.014.1734814
CAAGACG3850.014.1261484
Produced by FastQC (version 0.11.5)