##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062991_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1376412
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14217472675333	32.0	32.0	32.0	32.0	32.0
2	30.769453477592464	32.0	32.0	32.0	32.0	32.0
3	30.835587019003032	32.0	32.0	32.0	32.0	32.0
4	30.89490283432577	32.0	32.0	32.0	32.0	32.0
5	30.816405262377835	32.0	32.0	32.0	32.0	32.0
6	34.44193526356934	36.0	36.0	36.0	32.0	36.0
7	34.361414314899896	36.0	36.0	36.0	32.0	36.0
8	34.34402998520792	36.0	36.0	36.0	32.0	36.0
9	34.46132335376326	36.0	36.0	36.0	32.0	36.0
10	34.20867298454242	36.0	36.0	36.0	32.0	36.0
11	34.49177499179025	36.0	36.0	36.0	32.0	36.0
12	34.30395768127566	36.0	36.0	36.0	32.0	36.0
13	34.38142867106651	36.0	36.0	36.0	32.0	36.0
14	34.29549873148447	36.0	36.0	36.0	32.0	36.0
15	34.24551151835352	36.0	36.0	36.0	32.0	36.0
16	34.26222889658038	36.0	36.0	36.0	32.0	36.0
17	34.19271264708532	36.0	36.0	36.0	32.0	36.0
18	34.215909916507556	36.0	36.0	36.0	32.0	36.0
19	34.19884307896182	36.0	36.0	36.0	32.0	36.0
20	34.16377218449127	36.0	36.0	36.0	32.0	36.0
21	34.14908254214581	36.0	36.0	36.0	32.0	36.0
22	34.13335396669021	36.0	36.0	36.0	32.0	36.0
23	34.08625324394149	36.0	36.0	36.0	32.0	36.0
24	34.087674330069774	36.0	36.0	36.0	32.0	36.0
25	34.05919739147871	36.0	36.0	36.0	32.0	36.0
26	34.01601264737593	36.0	36.0	36.0	32.0	36.0
27	34.032322444151895	36.0	36.0	36.0	32.0	36.0
28	34.018739301895074	36.0	36.0	36.0	32.0	36.0
29	33.98174020569422	36.0	36.0	36.0	32.0	36.0
30	33.96094991906493	36.0	36.0	36.0	32.0	36.0
31	33.972140609061825	36.0	36.0	36.0	32.0	36.0
32	33.922067665786116	36.0	36.0	36.0	32.0	36.0
33	33.875505299285386	36.0	36.0	36.0	32.0	36.0
34	33.876497734689906	36.0	36.0	36.0	32.0	36.0
35	33.84437072620698	36.0	36.0	36.0	32.0	36.0
36	33.78636629148831	36.0	36.0	36.0	32.0	36.0
37	33.791446892354905	36.0	36.0	36.0	32.0	36.0
38	33.1989767598655	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	5.0
10	15.0
11	14.0
12	7.0
13	3.0
14	242.0
15	479.0
16	536.0
17	647.0
18	820.0
19	1043.0
20	1467.0
21	2086.0
22	3005.0
23	4548.0
24	6881.0
25	10079.0
26	14592.0
27	20524.0
28	28591.0
29	37671.0
30	50231.0
31	66882.0
32	90816.0
33	133309.0
34	290234.0
35	611681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73721849333055	17.248259803404522	11.34668557017126	25.66783613309367
2	16.4867741175718	20.34891497460084	37.70168852806993	25.462622379757438
3	18.927995593865415	24.94886515150084	28.47420878131578	27.64893047331796
4	12.496131150018382	15.983399909036617	36.40002964301958	35.12043929792543
5	14.366441951483896	36.855614864477054	33.52550005122024	15.252443132818804
6	32.93483172842897	36.00202265901195	17.577371792915752	13.485773819643331
7	29.467485026285733	31.04230419380244	21.331839594540007	18.15837118537182
8	27.845336781918178	32.44861632798367	20.09299554638516	19.613051343712993
9	28.000470790295616	14.434590299830937	18.694443294108073	38.87049561576537
10	15.715979564198987	26.821116994379572	31.875286978860917	25.58761646256052
11	36.5648419591902	21.746095750825532	22.638796557649204	19.050265732335067
12	25.048787469721255	24.17489962786386	28.796377158019094	21.9799357443958
13	29.475288906457088	19.96288394912456	25.275536540563166	25.28629060385519
14	23.912000976539794	19.878906159175987	25.785228091794743	30.423864772489477
15	25.074485865852775	26.775379696243007	23.228980452002034	24.921153985902187
16	25.28891559559301	26.144418197922352	24.060374912716792	24.506291293767852
17	23.766729790180925	25.74879844899276	25.594816798896623	24.88965496192969
18	24.500070113336577	25.06337446406891	26.60906474621515	23.827490676379362
19	25.181771878925797	25.28320229482683	26.218454668378488	23.316571157868886
20	25.639104900754827	24.259720897249878	25.68785742466064	24.413316777334654
21	26.82970423496126	24.421050490108108	24.98232617346579	23.76691910146484
22	25.762814816644713	24.439064546903076	25.265836286262537	24.532284350189673
23	24.141040349868888	24.5386318405972	25.922645494726027	25.397682314807884
24	24.385946172967607	25.34356713991846	25.756218287297916	24.514268399816018
25	24.599383952686868	24.712449489279482	25.826028761954174	24.862137796079477
26	24.278625185934455	25.492149514917266	26.159369897475358	24.069855401672918
27	25.048554270705043	25.194671200578366	25.268710559872705	24.48806396884389
28	24.058404427994947	25.055764080521154	26.158040126887805	24.727791364596094
29	24.109100130279007	25.43971889379827	26.08145017318461	24.36973080273811
30	24.1942936855642	25.38256428140689	26.237203658899862	24.185938374129048
31	24.57230709317043	25.50696122609417	25.097396133798526	24.82333554693687
32	24.13748506892272	25.4830984564681	25.174377544822296	25.205038929786884
33	23.90878977078282	25.087245230485077	25.70622679909002	25.297738199642083
34	24.599546740315915	25.292733240181857	25.796492360052987	24.311227659449244
35	25.391965016890627	25.046159480368868	25.59280689605561	23.969068606684896
36	24.248937485876137	25.791935428787756	25.290706334829466	24.66842075050664
37	24.985522662754697	25.819867774864147	25.172547335625	24.02206222675615
38	24.078251866383305	25.6406735327775	25.706718979892102	24.574355620947088
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	827.0
1	693.5
2	560.0
3	560.0
4	997.5
5	1435.0
6	1435.0
7	1823.0
8	2211.0
9	2053.5
10	1896.0
11	1896.0
12	2444.0
13	2992.0
14	3728.5
15	4465.0
16	4465.0
17	6221.5
18	7978.0
19	7978.0
20	9560.5
21	11143.0
22	11276.0
23	11409.0
24	11409.0
25	12982.0
26	14555.0
27	14555.0
28	18733.0
29	22911.0
30	28013.5
31	33116.0
32	33116.0
33	40634.5
34	48153.0
35	48153.0
36	53740.5
37	59328.0
38	67565.5
39	75803.0
40	75803.0
41	82073.5
42	88344.0
43	99479.5
44	110615.0
45	110615.0
46	115698.5
47	120782.0
48	120782.0
49	126588.5
50	132395.0
51	130504.0
52	128613.0
53	128613.0
54	122117.5
55	115622.0
56	115622.0
57	111346.5
58	107071.0
59	94477.5
60	81884.0
61	81884.0
62	76488.5
63	71093.0
64	58192.5
65	45292.0
66	45292.0
67	37378.0
68	29464.0
69	29464.0
70	23645.5
71	17827.0
72	14101.0
73	10375.0
74	10375.0
75	7812.5
76	5250.0
77	5250.0
78	4986.5
79	4723.0
80	3692.5
81	2662.0
82	2662.0
83	2706.5
84	2751.0
85	2751.0
86	1800.0
87	849.0
88	771.5
89	694.0
90	694.0
91	507.5
92	321.0
93	264.5
94	208.0
95	208.0
96	195.5
97	183.0
98	183.0
99	397.5
100	612.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12430870989209628
2	0.05637846807496592
3	0.010825247091713818
4	0.0024701906115320122
5	2.1795799513517754E-4
6	4.3591599027035507E-4
7	0.0
8	1.4530533009011836E-4
9	2.1795799513517754E-4
10	2.906106601802367E-4
11	0.001961621956216598
12	0.0024701906115320122
13	0.013658701028471125
14	0.00900893046558734
15	0.022667631494058465
16	0.010534636431533581
17	0.019906830222346215
18	0.005013033888109084
19	0.007192613839460859
20	0.00530364454828932
21	0.0060301711987399125
22	0.006902003179280622
23	0.009081583130632398
24	0.013731353693516185
25	0.015910933644867963
26	0.018526429586490093
27	0.008500361810271926
28	0.005666907873514616
29	0.009808109781082989
30	0.003051411931892486
31	0.005376297213334379
32	0.006393434523965208
33	0.008645667140362042
34	0.011551773742164411
35	0.014893796334237132
36	0.01416726968378654
37	0.00893627780054228
38	0.006320781858920149
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1376412.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.57059726182955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11022968334613	46.335334020740994
2	13.174246398022513	15.432469600133702
3	3.8892015405990716	6.833785713135458
4	1.5068482671435575	3.530280119582042
5	0.6851840409930344	2.0065819257617967
6	0.40507693536072953	1.4235358824641668
7	0.24104390947674734	0.9882660021065627
8	0.16011099235283446	0.750223716023179
9	0.12306110941751713	0.6486986410458601
>10	0.5660503215693694	6.131511777248589
>50	0.06118464435858923	2.5496943806910215
>100	0.06791730780902151	8.245740497756614
>500	0.007227864227258192	2.7582811846707522
>1k	0.002492366974916618	1.9745176662533261
>5k	1.2461834874583088E-4	0.3910788723859722
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5358	0.3892729793114271	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3243	0.23561259274112695	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1873	0.13607844162939586	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1656	0.120312813314618	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1389	0.10091455174758719	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.4530533009011836E-4
7	0.0	0.0	0.0	0.0	1.4530533009011836E-4
8	0.0	0.0	0.0	0.0	1.4530533009011836E-4
9	0.0	0.0	0.0	0.0	1.4530533009011836E-4
10	0.0	0.0	0.0	0.0	1.4530533009011836E-4
11	0.0	0.0	0.0	0.0	1.4530533009011836E-4
12	0.0	0.0	0.0	0.0	1.4530533009011836E-4
13	0.0	0.0	0.0	0.0	2.906106601802367E-4
14	0.0	0.0	0.0	7.265266504505918E-5	2.906106601802367E-4
15	0.0	0.0	0.0	7.265266504505918E-5	3.632633252252959E-4
16	0.0	0.0	0.0	7.265266504505918E-5	3.632633252252959E-4
17	0.0	0.0	0.0	7.265266504505918E-5	3.632633252252959E-4
18	0.0	0.0	0.0	1.4530533009011836E-4	3.632633252252959E-4
19	0.0	0.0	0.0	1.4530533009011836E-4	3.632633252252959E-4
20	0.0	0.0	0.0	2.906106601802367E-4	3.632633252252959E-4
21	0.0	0.0	0.0	2.906106601802367E-4	3.632633252252959E-4
22	0.0	0.0	0.0	4.3591599027035507E-4	3.632633252252959E-4
23	0.0	0.0	0.0	0.0010171373106308286	3.632633252252959E-4
24	0.0	0.0	0.0	0.0017436639610814203	3.632633252252959E-4
25	0.0	0.0	0.0	0.002397537946486953	3.632633252252959E-4
26	0.0	0.0	0.0	0.0034146752571177818	4.3591599027035507E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	285	0.0	18.524128	28
CTAGCGG	290	0.0	18.20607	29
AAACCGG	45	0.0088497475	17.778261	10
CGACTCA	45	0.0088497475	17.778261	10
TAGCGGC	320	0.0	16.999846	30
ACCGTCG	245	0.0	16.97697	8
TACCGTC	275	0.0	16.288393	7
ATCGTTT	240	0.0	15.999272	29
ATAATAC	155	1.891749E-10	15.480918	3
CGTCGTA	280	0.0	15.428992	10
ATACCGT	280	0.0	15.424507	6
CCGTCGT	260	0.0	15.385035	9
CGTTTAT	260	0.0	15.384476	31
GTCTTAC	140	1.2656528E-8	14.868894	1
GTATAGA	185	1.0913936E-11	14.714331	1
GTATAAT	185	1.0913936E-11	14.714331	1
GCCTTAT	110	3.5144985E-6	14.556959	1
TACGCTA	315	0.0	14.2226095	9
TAATATA	395	0.0	14.173481	4
CAAGACG	385	0.0	14.126148	4
>>END_MODULE