FastQCFastQC Report
Thu 2 Feb 2017
SRR4062991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376412
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT67350.48931569907847355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT44170.3209068215040264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT26650.19361935234508273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.14516002476002823No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14790.10745329160164252No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13790.10018802509713662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA604.7231697E-621.33255830
GTATTAC652.0990346E-417.2326471
GGTCGTA957.698036E-716.84149429
ACCGTCG2500.016.0017438
CTAGCGG2750.015.7085229
CGTCGTA2600.015.38461510
TAGCGGC2850.015.15734430
TCTAGCG2750.015.12672328
TACCGTC2800.014.8587627
TAACGAA1401.27965905E-814.85660313
ATACCGT2600.014.770846
AACCGCG2650.014.49214657
ACGAACG1452.0314474E-814.34430715
CCGTCGT2800.014.2862339
TAGACTG2053.6379788E-1214.0503115
TCTAGAT2850.014.0355982
CGCGGTC2650.013.88679210
CGAAAAC2650.013.88628923
GAACCGC3150.013.715786
GTATTAT1402.0534935E-713.7157791
Produced by FastQC (version 0.11.5)