FastQCFastQC Report
Thu 2 Feb 2017
SRR4062990_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062990_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923682
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33130.3586732230356335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21650.23438802531607197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12390.1341370731485511No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10740.11627378253554796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.11237633731089271No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10100.10934499102504974No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9820.10631364473920678No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9680.10479797159628532No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9410.10187488767779387No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9300.100684001636927No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9280.10046747690222392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT1750.021.025329
CTATATA659.324647E-619.7180631
TATACAC1400.019.427653
CATCGTT1950.018.86885828
CGTTTAT2150.018.60175731
GGCATCG1950.018.04945226
GCATCGT2200.017.45182827
GTAATAC1001.3143344E-615.9992423
ACCGTGT703.701323E-415.9966448
CGGCATC2300.015.30279425
CGCATCG2550.015.06055713
ACAATAC1704.7293724E-1115.058113
AAAGGCG859.445871E-515.0556655
CGGTCCA2350.014.97963610
ATTTCGG756.237259E-414.93505214
TCTAGCG2250.014.93100928
AAGACGG2150.014.8805995
AACGAAT650.004165063614.766932531
GGCCCGT650.004166562614.7661326
ATAATAC1002.3257537E-514.3993183
Produced by FastQC (version 0.11.5)