##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062990_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	923682
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.152050164450536	32.0	32.0	32.0	32.0	32.0
2	30.76256980216135	32.0	32.0	32.0	32.0	32.0
3	30.8212350137818	32.0	32.0	32.0	32.0	32.0
4	30.87300174735461	32.0	32.0	32.0	32.0	32.0
5	30.809280683178844	32.0	32.0	32.0	32.0	32.0
6	34.44600306165975	36.0	36.0	36.0	32.0	36.0
7	34.337645423424945	36.0	36.0	36.0	32.0	36.0
8	34.34184816852553	36.0	36.0	36.0	32.0	36.0
9	34.45678166295327	36.0	36.0	36.0	32.0	36.0
10	34.18701457861039	36.0	36.0	36.0	32.0	36.0
11	34.465890858542224	36.0	36.0	36.0	32.0	36.0
12	34.29594709001583	36.0	36.0	36.0	32.0	36.0
13	34.36670845594046	36.0	36.0	36.0	32.0	36.0
14	34.271704980718475	36.0	36.0	36.0	32.0	36.0
15	34.21356917207437	36.0	36.0	36.0	32.0	36.0
16	34.23096909975511	36.0	36.0	36.0	32.0	36.0
17	34.153247546233445	36.0	36.0	36.0	32.0	36.0
18	34.1796072674362	36.0	36.0	36.0	32.0	36.0
19	34.16698928852137	36.0	36.0	36.0	32.0	36.0
20	34.133094506550954	36.0	36.0	36.0	32.0	36.0
21	34.12334872824197	36.0	36.0	36.0	32.0	36.0
22	34.10075978529407	36.0	36.0	36.0	32.0	36.0
23	34.04291845028917	36.0	36.0	36.0	32.0	36.0
24	34.03194281148707	36.0	36.0	36.0	32.0	36.0
25	34.01230726592052	36.0	36.0	36.0	32.0	36.0
26	33.97762649916313	36.0	36.0	36.0	32.0	36.0
27	33.97158545906492	36.0	36.0	36.0	32.0	36.0
28	33.95487841053523	36.0	36.0	36.0	32.0	36.0
29	33.91367591876858	36.0	36.0	36.0	32.0	36.0
30	33.88476012307266	36.0	36.0	36.0	32.0	36.0
31	33.90345703391427	36.0	36.0	36.0	32.0	36.0
32	33.85730803458333	36.0	36.0	36.0	32.0	36.0
33	33.81923973835151	36.0	36.0	36.0	32.0	36.0
34	33.81742417845103	36.0	36.0	36.0	32.0	36.0
35	33.76488012108063	36.0	36.0	36.0	32.0	36.0
36	33.7204600717563	36.0	36.0	36.0	27.0	36.0
37	33.72152104295634	36.0	36.0	36.0	27.0	36.0
38	33.118444443000946	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	10.0
11	10.0
12	4.0
13	5.0
14	236.0
15	426.0
16	520.0
17	544.0
18	701.0
19	839.0
20	1075.0
21	1450.0
22	2213.0
23	3219.0
24	4650.0
25	6719.0
26	9688.0
27	13727.0
28	19284.0
29	25763.0
30	34144.0
31	45628.0
32	61615.0
33	90518.0
34	198328.0
35	402362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.52030185865063	17.454969969139917	11.575078234157244	25.449649938052204
2	16.436409328726022	20.166816512668305	37.65189510057736	25.744879058028314
3	18.958410254133693	24.405756364711245	28.530515857504962	28.105317523650104
4	12.616196108066442	15.695162907322437	36.132252986507936	35.55638799810318
5	14.546797000263078	36.65724422176054	33.48872608617044	15.30723269180594
6	33.5024397003721	35.721392388481284	17.228712572224765	13.547455338921853
7	29.857894816614376	31.002877613724205	20.910984516316223	18.2282430533452
8	28.19891282813006	32.419417526180574	19.871037728393244	19.510631917296124
9	27.740174648851013	14.559880998005806	18.85042687851447	38.84951747462871
10	15.920340377619954	26.674714186731336	31.449961025463363	25.954984410185343
11	36.689925459988196	21.695636404973666	22.560452111967	19.053986023071133
12	25.174333817999933	24.021310895038102	28.47208764273395	22.33226764422801
13	29.365154986552096	19.674759951665504	25.38210670836022	25.577978353422175
14	23.93247344379157	20.047054645536466	25.437767362092316	30.582704548579642
15	25.07769473494996	26.76164013314766	23.249296686685017	24.91136844521736
16	25.494673496544458	26.039504584831562	24.022914954595606	24.44290696402837
17	23.77278525284944	25.895438509865578	25.54926075836425	24.78251547892073
18	24.703778128085645	24.881338022069396	26.753728757773658	23.6611550920713
19	25.362869609467197	25.289245460746418	25.993980143134003	23.353904786652375
20	25.50943404397682	24.323127509817315	25.760185703474253	24.40725274273161
21	26.817860456656206	24.36687104209816	24.87746676959422	23.937801731651415
22	25.515416479901955	24.394178570887224	25.44861425994617	24.641790689264656
23	23.990346564401168	24.406973080600125	25.96434205383447	25.638338301164236
24	24.44200223047522	25.495847634721784	25.581060450209513	24.481089684593478
25	24.717419762804763	24.694356407877056	25.6453678929947	24.942855936323486
26	24.191395020221215	25.53384549583936	26.21806428559827	24.056695198341156
27	25.117556683272035	25.21879131266126	25.22214775491908	24.441504249147624
28	24.142489495286547	24.867452765329954	26.169160261705677	24.82089747767782
29	24.03881342315596	25.36904584028982	26.103889354459408	24.48825138209481
30	24.136769977469893	25.42231768856919	26.284221455460006	24.156690878500907
31	24.604252987929186	25.284284991982748	25.239678530325353	24.87178348976271
32	24.090972083878288	25.500636622694948	25.17171489697106	25.2366763964557
33	24.01863128080311	24.864552927215875	25.735485184190694	25.38133060779032
34	24.662830835062127	25.169017683515744	25.90832117041227	24.25983031100986
35	25.42978321623047	24.91253828987588	25.81449563476466	23.84318285912899
36	24.228066053313437	25.777293659189414	25.358586421845104	24.636053865652038
37	25.052403864893307	25.541470513081478	25.240256562393082	24.165869059632133
38	24.095501135191142	25.58656955738914	25.696461689870326	24.621467617549396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1074.0
1	728.5
2	383.0
3	383.0
4	764.0
5	1145.0
6	1145.0
7	1269.5
8	1394.0
9	1335.0
10	1276.0
11	1276.0
12	1584.5
13	1893.0
14	2418.5
15	2944.0
16	2944.0
17	4200.5
18	5457.0
19	5457.0
20	6485.5
21	7514.0
22	7723.0
23	7932.0
24	7932.0
25	9211.0
26	10490.0
27	10490.0
28	13131.5
29	15773.0
30	19046.5
31	22320.0
32	22320.0
33	27509.5
34	32699.0
35	32699.0
36	36240.5
37	39782.0
38	44986.5
39	50191.0
40	50191.0
41	54220.0
42	58249.0
43	65602.0
44	72955.0
45	72955.0
46	75648.0
47	78341.0
48	78341.0
49	81620.0
50	84899.0
51	84777.5
52	84656.0
53	84656.0
54	80935.0
55	77214.0
56	77214.0
57	74518.0
58	71822.0
59	64388.5
60	56955.0
61	56955.0
62	53133.5
63	49312.0
64	40833.5
65	32355.0
66	32355.0
67	26786.5
68	21218.0
69	21218.0
70	17070.5
71	12923.0
72	10076.5
73	7230.0
74	7230.0
75	5496.0
76	3762.0
77	3762.0
78	3587.0
79	3412.0
80	2705.5
81	1999.0
82	1999.0
83	2017.5
84	2036.0
85	2036.0
86	1340.0
87	644.0
88	542.0
89	440.0
90	440.0
91	344.5
92	249.0
93	213.5
94	178.0
95	178.0
96	154.0
97	130.0
98	130.0
99	283.0
100	436.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12244473747458542
2	0.05543033208398561
3	0.012233647510723387
4	0.0030313462858429633
5	1.0826236735153439E-4
6	3.247871020546032E-4
7	0.0
8	1.0826236735153439E-4
9	0.0
10	2.1652473470306878E-4
11	0.001840460244976085
12	0.0024900344490852914
13	0.012774959347481058
14	0.00811967755136508
15	0.021219424000900742
16	0.010068400163692698
17	0.01818807771505778
18	0.0054131183675767205
19	0.007794890449310477
20	0.006387479673740529
21	0.0059544302043343916
22	0.006495742041092064
23	0.00790315281666201
24	0.012125385143371853
25	0.015048469061863284
26	0.015914568000675557
27	0.009202301224880425
28	0.0053048560002251855
29	0.00909403885752889
30	0.0031396086531944975
31	0.0053048560002251855
32	0.006279217306388995
33	0.008011415184013547
34	0.011908860408668783
35	0.014615419592457145
36	0.0135327959189418
37	0.009310563592231959
38	0.0053048560002251855
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	923682.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.439508642962956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.86842666502884	47.843506276767314
2	11.243306146562464	13.141065734550311
3	3.1682813439206514	5.554584149441676
4	1.1892865528236194	2.780052871307821
5	0.6738057606854818	1.9688438787628726
6	0.3800948480762738	1.3327533695579459
7	0.26009622829446916	1.0639927046991688
8	0.18217539609185945	0.8516992507556337
9	0.1428513846292609	0.7513348254030825
>10	0.7216220988813005	7.842950739075934
>50	0.0802904521006799	3.316596854238564
>100	0.08177178903759068	9.594224045819274
>500	0.0068762640254806705	2.889812114366029
>1k	0.0011150698419698385	1.0685831852543355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3313	0.3586732230356335	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2165	0.23438802531607197	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1239	0.1341370731485511	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1074	0.11627378253554796	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1038	0.11237633731089271	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1010	0.10934499102504974	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	982	0.10631364473920678	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	968	0.10479797159628532	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	941	0.10187488767779387	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	930	0.100684001636927	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	928	0.10046747690222392	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.247871020546032E-4
2	0.0	0.0	0.0	0.0	3.247871020546032E-4
3	0.0	0.0	0.0	0.0	3.247871020546032E-4
4	0.0	0.0	0.0	0.0	3.247871020546032E-4
5	0.0	0.0	0.0	0.0	3.247871020546032E-4
6	0.0	0.0	0.0	0.0	3.247871020546032E-4
7	0.0	0.0	0.0	0.0	3.247871020546032E-4
8	0.0	0.0	0.0	0.0	3.247871020546032E-4
9	0.0	0.0	0.0	0.0	3.247871020546032E-4
10	0.0	0.0	0.0	0.0	3.247871020546032E-4
11	0.0	0.0	0.0	0.0	3.247871020546032E-4
12	0.0	0.0	0.0	0.0	7.578365714607408E-4
13	0.0	0.0	0.0	0.0	8.660989388122752E-4
14	0.0	0.0	0.0	0.0	0.001082623673515344
15	0.0	0.0	0.0	1.082623673515344E-4	0.0012991484082184128
16	0.0	0.0	0.0	1.082623673515344E-4	0.0012991484082184128
17	0.0	0.0	0.0	2.165247347030688E-4	0.0012991484082184128
18	0.0	0.0	0.0	2.165247347030688E-4	0.0012991484082184128
19	0.0	0.0	0.0	3.247871020546032E-4	0.0012991484082184128
20	0.0	0.0	0.0	4.330494694061376E-4	0.0012991484082184128
21	0.0	0.0	0.0	7.578365714607408E-4	0.0014074107755699472
22	0.0	0.0	0.0	0.0011908860408668784	0.0014074107755699472
23	0.0	0.0	0.0	0.0020569849796791538	0.0014074107755699472
24	0.0	0.0	0.0	0.004222232326709842	0.0014074107755699472
25	0.0	0.0	0.0	0.005846167836982857	0.0014074107755699472
26	0.0	0.0	0.0	0.007037053877849736	0.0014074107755699472
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	175	0.0	21.0253	29
CTATATA	65	9.324647E-6	19.718063	1
TATACAC	140	0.0	19.42765	3
CATCGTT	195	0.0	18.868858	28
CGTTTAT	215	0.0	18.601757	31
GGCATCG	195	0.0	18.049452	26
GCATCGT	220	0.0	17.451828	27
GTAATAC	100	1.3143344E-6	15.999242	3
ACCGTGT	70	3.701323E-4	15.996644	8
CGGCATC	230	0.0	15.302794	25
CGCATCG	255	0.0	15.060557	13
ACAATAC	170	4.7293724E-11	15.05811	3
AAAGGCG	85	9.445871E-5	15.055665	5
CGGTCCA	235	0.0	14.979636	10
ATTTCGG	75	6.237259E-4	14.935052	14
TCTAGCG	225	0.0	14.931009	28
AAGACGG	215	0.0	14.880599	5
AACGAAT	65	0.0041650636	14.7669325	31
GGCCCGT	65	0.0041665626	14.766132	6
ATAATAC	100	2.3257537E-5	14.399318	3
>>END_MODULE