FastQCFastQC Report
Thu 2 Feb 2017
SRR4062990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923682
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44380.4804683863061097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT30140.32630277519752465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17760.1922739644163251No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.16001177894556784No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11550.12504303429102223No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11170.12092906433166394No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10750.11638204490289948No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10660.11540768359673567No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10280.11129371363737736No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10070.10902020392299515No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9870.10685495657596444No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9660.10458144686158223No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9490.10274098661660616No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9270.1003592145348724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA802.7935766E-618.0009441
TGTGCGA450.00884908217.77774810
ACCGTCC450.00884908217.7777488
GGACCGT450.00884908217.7777486
TCTAGCG2800.017.71425628
CTAGCGG2800.017.71425629
AATCGCT1006.8099325E-817.59997215
ACGAACG1302.582965E-1017.2307415
TAGCGGC2950.016.8135330
CCGATAA1456.91216E-1116.5516979
CGATAAC1456.91216E-1116.55169710
GTATTAG2350.016.3412821
AGGGGCG2350.016.34039732
CGGTCCA3050.016.26226810
GTATAAC600.002440563616.000841
ATCGCTC1001.3137087E-615.99997416
TAATACT1203.1075615E-815.9999734
TTGGACT908.5772845E-615.9999734
GATAACG1501.1459633E-1015.99997211
ATAATAC703.695463E-415.9999723
Produced by FastQC (version 0.11.5)