##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062990_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	923682
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.157109264876873	32.0	32.0	32.0	32.0	32.0
2	31.276255248018256	32.0	32.0	32.0	32.0	32.0
3	31.361110208924718	32.0	32.0	32.0	32.0	32.0
4	31.457240695390837	32.0	32.0	32.0	32.0	32.0
5	31.38191498805866	32.0	32.0	32.0	32.0	32.0
6	34.88432382573223	36.0	36.0	36.0	36.0	36.0
7	34.906570659599296	36.0	36.0	36.0	36.0	36.0
8	34.849881236183016	36.0	36.0	36.0	32.0	36.0
9	34.954363081666635	36.0	36.0	36.0	32.0	36.0
10	34.81094684101238	36.0	36.0	36.0	32.0	36.0
11	34.98304611327275	36.0	36.0	36.0	36.0	36.0
12	34.891001448550476	36.0	36.0	36.0	32.0	36.0
13	34.928933334199435	36.0	36.0	36.0	36.0	36.0
14	34.86596577610043	36.0	36.0	36.0	32.0	36.0
15	34.84695382176983	36.0	36.0	36.0	32.0	36.0
16	34.85574256075143	36.0	36.0	36.0	32.0	36.0
17	34.816017850299126	36.0	36.0	36.0	32.0	36.0
18	34.81271584809491	36.0	36.0	36.0	32.0	36.0
19	34.81153145779608	36.0	36.0	36.0	32.0	36.0
20	34.79629677746237	36.0	36.0	36.0	32.0	36.0
21	34.7759716006158	36.0	36.0	36.0	32.0	36.0
22	34.76619442622028	36.0	36.0	36.0	32.0	36.0
23	34.71068073211343	36.0	36.0	36.0	32.0	36.0
24	34.67582241507358	36.0	36.0	36.0	32.0	36.0
25	34.67127864351584	36.0	36.0	36.0	32.0	36.0
26	34.60191819262474	36.0	36.0	36.0	32.0	36.0
27	34.580095747237685	36.0	36.0	36.0	32.0	36.0
28	34.548226554160415	36.0	36.0	36.0	32.0	36.0
29	34.514790804627566	36.0	36.0	36.0	32.0	36.0
30	34.48834663877828	36.0	36.0	36.0	32.0	36.0
31	34.47965750117465	36.0	36.0	36.0	32.0	36.0
32	34.443482713747805	36.0	36.0	36.0	32.0	36.0
33	34.40160791267991	36.0	36.0	36.0	32.0	36.0
34	34.388220188333214	36.0	36.0	36.0	32.0	36.0
35	34.347299178721684	36.0	36.0	36.0	32.0	36.0
36	34.31292262921655	36.0	36.0	36.0	32.0	36.0
37	34.30180300146587	36.0	36.0	36.0	32.0	36.0
38	33.871173195970044	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	9.0
21	30.0
22	69.0
23	249.0
24	668.0
25	1664.0
26	3416.0
27	6759.0
28	11879.0
29	18375.0
30	27299.0
31	38953.0
32	55147.0
33	85944.0
34	207009.0
35	466208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.897048123895644	18.60665038284309	12.291580050583791	26.204721442677474
2	15.476863249473304	21.22483711926832	36.82825907617557	26.470040555082807
3	17.850515653655698	25.12942766016876	28.91882704220717	28.101229643968377
4	12.029638066120517	16.618363345628396	36.23571876316071	35.11627982509037
5	14.071957742987026	37.114544956538026	33.18082758008447	15.632669720390483
6	33.42647515754898	36.15988636769621	16.845642597390224	13.567995877364588
7	29.638110209282665	31.15097344423766	20.907046712115356	18.303869634364318
8	27.976389606143382	32.7261841736308	19.661975551636367	19.63545066858945
9	27.585982895989037	14.307367673440929	18.669117830157322	39.43753160041271
10	15.782909480658677	26.80860047419533	31.480398843742897	25.9280912014031
11	36.8848421200786	21.5326234646428	22.396050430630815	19.186483984647783
12	24.877365405081203	24.280188247215754	28.55494170593897	22.287504641764073
13	29.524510653040014	19.6568075523991	25.39602459726312	25.422657197297767
14	23.806894580602414	19.89624134713029	25.52599271177743	30.770871360489867
15	25.02647014881745	26.928639943183907	23.10156525730717	24.943324650691473
16	25.326384325324437	25.982563244236918	24.02138833644949	24.66966409398916
17	23.69408519382212	25.822848123055337	25.651901845007263	24.83116483811528
18	24.6700704354962	24.637375200556036	26.882520174692154	23.81003418925561
19	25.24180399747965	25.163746830619193	26.16181759523299	23.43263157666816
20	25.53920072059432	24.096821200369824	25.9824268525315	24.38155122650436
21	26.580605208941183	24.358084663428176	25.153272612514492	23.90803751511615
22	25.38211202556724	24.289961263724962	25.724870680602198	24.6030560301056
23	23.93358746147209	24.018140461912715	26.278119827083156	25.770152249532035
24	24.34344287319662	25.24180399747965	25.896250008119676	24.51850312120405
25	24.62719853802499	24.458200982589247	25.988056495633778	24.926543983751984
26	24.336838868788178	25.160932009068055	26.50814890839055	23.994080213753218
27	25.184695998614238	24.79126970379352	25.50959206651654	24.514442231075698
28	24.10158474453329	24.62081105835125	26.562496616801017	24.715107580314438
29	24.172604857515896	24.92860096873166	26.383430661201583	24.515363512550856
30	24.28638860560236	24.98695438473414	26.54831424667797	24.17834276298553
31	24.571659943573653	24.89601399615885	25.455513910631584	25.076812149635913
32	24.206057929027523	24.93758674522184	25.61379349169953	25.242561834051113
33	24.092598968043113	24.53463421393943	26.167122451233215	25.20564436678424
34	24.693130319742078	24.7935977966443	26.233920331889117	24.279351551724513
35	25.41166765185421	24.572309517777764	26.001914078654774	24.014108751713252
36	24.301437074664225	25.292687310134877	25.706249553417738	24.699626061783167
37	25.128453298862595	25.115028765311003	25.578066910473517	24.17845102535288
38	24.266819388944885	25.022383268682585	25.934386438608136	24.776410903764397
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	429.0
1	341.0
2	253.0
3	253.0
4	676.5
5	1100.0
6	1100.0
7	1297.5
8	1495.0
9	1365.0
10	1235.0
11	1235.0
12	1619.0
13	2003.0
14	2613.5
15	3224.0
16	3224.0
17	4744.5
18	6265.0
19	6265.0
20	7252.0
21	8239.0
22	8283.5
23	8328.0
24	8328.0
25	9313.5
26	10299.0
27	10299.0
28	13109.5
29	15920.0
30	19049.0
31	22178.0
32	22178.0
33	27353.0
34	32528.0
35	32528.0
36	35996.0
37	39464.0
38	44721.0
39	49978.0
40	49978.0
41	53760.5
42	57543.0
43	64877.0
44	72211.0
45	72211.0
46	75667.5
47	79124.0
48	79124.0
49	82744.5
50	86365.0
51	85429.0
52	84493.0
53	84493.0
54	80708.5
55	76924.0
56	76924.0
57	74528.5
58	72133.0
59	64592.5
60	57052.0
61	57052.0
62	53412.5
63	49773.0
64	40966.0
65	32159.0
66	32159.0
67	26768.5
68	21378.0
69	21378.0
70	17105.5
71	12833.0
72	10021.0
73	7209.0
74	7209.0
75	5393.5
76	3578.0
77	3578.0
78	3589.0
79	3600.0
80	2700.5
81	1801.0
82	1801.0
83	1774.0
84	1747.0
85	1747.0
86	1074.0
87	401.0
88	360.0
89	319.0
90	319.0
91	189.0
92	59.0
93	40.5
94	22.0
95	22.0
96	16.5
97	11.0
98	11.0
99	10.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00714531624520127
2	0.0
3	0.0
4	5.41311836757672E-4
5	1.0826236735153439E-4
6	1.0826236735153439E-4
7	3.247871020546032E-4
8	0.0022735097143822226
9	0.004222232326709842
10	0.0012991484082184128
11	0.004005707592006773
12	3.247871020546032E-4
13	2.1652473470306878E-4
14	0.0
15	0.0
16	1.0826236735153439E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0826236735153439E-4
22	0.0
23	1.0826236735153439E-4
24	0.0
25	0.0
26	0.0
27	2.1652473470306878E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0826236735153439E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	923682.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.35545996833312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.53421729484691	45.38542858840786
2	12.052356656531739	13.58432206162495
3	3.3401142489143436	5.647010245330541
4	1.3785548085091401	3.107563613003599
5	0.6892407800700164	1.9421240594889249
6	0.4186666922374403	1.4156492408676904
7	0.28927837654930527	1.141169117853014
8	0.18909457085847328	0.8525209214595058
9	0.15410978612966178	0.781643509466268
>10	0.7712121169711501	8.081534339925428
>50	0.08386811172522671	3.331819017105335
>100	0.09061103234262222	10.276085547817823
>500	0.006747630022028042	2.5893927595918456
>1k	0.0019278942920080118	1.8637369780572535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4438	0.4804683863061097	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3014	0.32630277519752465	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1776	0.1922739644163251	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1478	0.16001177894556784	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1155	0.12504303429102223	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1117	0.12092906433166394	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1075	0.11638204490289948	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1066	0.11540768359673567	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1028	0.11129371363737736	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1007	0.10902020392299515	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	987	0.10685495657596444	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	966	0.10458144686158223	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	949	0.10274098661660616	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	927	0.1003592145348724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.082623673515344E-4
5	0.0	0.0	0.0	0.0	1.082623673515344E-4
6	0.0	0.0	0.0	0.0	2.165247347030688E-4
7	0.0	0.0	0.0	0.0	2.165247347030688E-4
8	0.0	0.0	0.0	0.0	2.165247347030688E-4
9	0.0	0.0	0.0	0.0	3.247871020546032E-4
10	0.0	0.0	0.0	0.0	3.247871020546032E-4
11	0.0	0.0	0.0	0.0	3.247871020546032E-4
12	0.0	0.0	0.0	0.0	6.495742041092064E-4
13	0.0	0.0	0.0	0.0	6.495742041092064E-4
14	0.0	0.0	0.0	0.0	6.495742041092064E-4
15	0.0	0.0	0.0	1.082623673515344E-4	0.001082623673515344
16	0.0	0.0	0.0	1.082623673515344E-4	0.001082623673515344
17	0.0	0.0	0.0	2.165247347030688E-4	0.001082623673515344
18	0.0	0.0	0.0	3.247871020546032E-4	0.001082623673515344
19	0.0	0.0	0.0	4.330494694061376E-4	0.001082623673515344
20	0.0	0.0	0.0	5.41311836757672E-4	0.0011908860408668784
21	0.0	0.0	0.0	7.578365714607408E-4	0.0011908860408668784
22	0.0	0.0	0.0	0.0011908860408668784	0.0011908860408668784
23	0.0	0.0	0.0	0.0020569849796791538	0.0011908860408668784
24	0.0	0.0	0.0	0.004005707592006773	0.0011908860408668784
25	0.0	0.0	0.0	0.005521380734928255	0.0011908860408668784
26	0.0	0.0	0.0	0.006712266775795133	0.0011908860408668784
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAA	80	2.7935766E-6	18.000944	1
TGTGCGA	45	0.008849082	17.777748	10
ACCGTCC	45	0.008849082	17.777748	8
GGACCGT	45	0.008849082	17.777748	6
TCTAGCG	280	0.0	17.714256	28
CTAGCGG	280	0.0	17.714256	29
AATCGCT	100	6.8099325E-8	17.599972	15
ACGAACG	130	2.582965E-10	17.23074	15
TAGCGGC	295	0.0	16.81353	30
CCGATAA	145	6.91216E-11	16.551697	9
CGATAAC	145	6.91216E-11	16.551697	10
GTATTAG	235	0.0	16.341282	1
AGGGGCG	235	0.0	16.340397	32
CGGTCCA	305	0.0	16.262268	10
GTATAAC	60	0.0024405636	16.00084	1
ATCGCTC	100	1.3137087E-6	15.999974	16
TAATACT	120	3.1075615E-8	15.999973	4
TTGGACT	90	8.5772845E-6	15.999973	4
GATAACG	150	1.1459633E-10	15.999972	11
ATAATAC	70	3.695463E-4	15.999972	3
>>END_MODULE