FastQCFastQC Report
Thu 2 Feb 2017
SRR4062989_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062989_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609449
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28970.17999949050886357No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT28030.1741589823598014No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25200.15657532484719927No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24860.15446280062307038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24750.15377933690349926No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24430.15179107881020149No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23480.14588843759572376No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23470.1458263045303082No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT23110.1435895141753482No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20960.13023090511100383No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20430.1269378526439794No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20230.12569519133566828No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG20010.12432826389652607No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19510.12122161062574832No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18960.11780429202789278No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG18550.115256836345855No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18490.11488403795336168No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT18490.11488403795336168No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18370.11413844116837502No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17690.10991339272011726No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16390.10183609421609506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA350.002067154522.85665317
TCTAGCG5950.021.51214628
CTAGCGG6300.020.31765729
TAAGCTA400.004488833719.9964685
TAGCGGC6450.019.84515430
TCCGTAC507.204709E-419.1972033
AGCGGCG7450.017.82567631
CGCTTAC450.00885444117.77684618
GTATCAA21850.017.6669831
CCTATTC6100.017.571213
CTATTCC6300.017.0128654
GTTTTCG6350.016.88152928
GTCCTAT6500.016.5104241
TTTTCGG6450.016.12418729
GATATAC908.496061E-616.0175761
ACGCGAA600.00244231716.00015411
TTCGGAA6600.016.00015431
AACCGCG2650.015.6968037
GGTATCA9800.015.5272431
CGTTATT3000.015.4716242
Produced by FastQC (version 0.11.5)