##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062989_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1609449
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27242491063712	32.0	32.0	32.0	32.0	32.0
2	30.938010461965554	32.0	32.0	32.0	32.0	32.0
3	30.976393163126016	32.0	32.0	32.0	32.0	32.0
4	31.032022760584525	32.0	32.0	32.0	32.0	32.0
5	30.963279979670062	32.0	32.0	32.0	32.0	32.0
6	34.56310948653856	36.0	36.0	36.0	32.0	36.0
7	34.51698749074994	36.0	36.0	36.0	32.0	36.0
8	34.51120041703714	36.0	36.0	36.0	32.0	36.0
9	34.63589899400354	36.0	36.0	36.0	32.0	36.0
10	34.37406714968912	36.0	36.0	36.0	32.0	36.0
11	34.5936913813361	36.0	36.0	36.0	32.0	36.0
12	34.44392149114386	36.0	36.0	36.0	32.0	36.0
13	34.528710757532544	36.0	36.0	36.0	32.0	36.0
14	34.418207100690985	36.0	36.0	36.0	32.0	36.0
15	34.36812412198212	36.0	36.0	36.0	32.0	36.0
16	34.38828070973358	36.0	36.0	36.0	32.0	36.0
17	34.304590577272094	36.0	36.0	36.0	32.0	36.0
18	34.33015522703733	36.0	36.0	36.0	32.0	36.0
19	34.32263650479139	36.0	36.0	36.0	32.0	36.0
20	34.30725981376235	36.0	36.0	36.0	32.0	36.0
21	34.28775251654448	36.0	36.0	36.0	32.0	36.0
22	34.27145812013925	36.0	36.0	36.0	32.0	36.0
23	34.21330467756356	36.0	36.0	36.0	32.0	36.0
24	34.20125024154229	36.0	36.0	36.0	32.0	36.0
25	34.17958692695451	36.0	36.0	36.0	32.0	36.0
26	34.12325149787287	36.0	36.0	36.0	32.0	36.0
27	34.12255250088695	36.0	36.0	36.0	32.0	36.0
28	34.10770021293001	36.0	36.0	36.0	32.0	36.0
29	34.082132456511516	36.0	36.0	36.0	32.0	36.0
30	34.05532763076059	36.0	36.0	36.0	32.0	36.0
31	34.06940760471441	36.0	36.0	36.0	32.0	36.0
32	34.04125324878266	36.0	36.0	36.0	32.0	36.0
33	33.99853614497881	36.0	36.0	36.0	32.0	36.0
34	34.01565442583145	36.0	36.0	36.0	32.0	36.0
35	33.97112055119485	36.0	36.0	36.0	32.0	36.0
36	33.934818065064505	36.0	36.0	36.0	32.0	36.0
37	33.93638692496624	36.0	36.0	36.0	32.0	36.0
38	33.29543589141377	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	5.0
9	4.0
10	18.0
11	10.0
12	6.0
13	6.0
14	159.0
15	390.0
16	516.0
17	565.0
18	758.0
19	921.0
20	1296.0
21	1806.0
22	2789.0
23	4087.0
24	6198.0
25	9214.0
26	13700.0
27	20115.0
28	28694.0
29	39622.0
30	54934.0
31	75140.0
32	106664.0
33	164446.0
34	367729.0
35	709656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.84697995261085	16.993407944183073	11.147958279135649	26.01165382407043
2	17.342803412958673	18.992213605209578	36.71808899180952	26.946893990022225
3	19.3808758290399	22.917760608883317	27.81920172304832	29.88216183902846
4	12.736486822352788	14.629686241760865	35.36242257945858	37.271404356427766
5	15.187940213041987	35.845457573031254	33.133533981837246	15.833068232089511
6	35.45698729311942	34.74959970623377	16.221450526672893	13.571962473973914
7	31.36802183108743	29.618167222550838	20.25104259348547	18.762768352876265
8	28.818842745365338	32.16241354949868	19.010442717281155	20.008300987854835
9	27.236995067249808	14.187233254652062	18.342635700336825	40.2331359777613
10	16.268391490207772	25.53695695397157	30.466870464260857	27.727781091559795
11	38.56993380232158	20.82635877973583	21.489640380211032	19.11406703773156
12	25.522568250293432	23.124169336904835	27.56847708503815	23.78478532776358
13	29.292891631401353	18.48188263952325	25.328946141943664	26.896279587131733
14	23.88177022780613	19.537664348691706	24.08129710490292	32.49926831859925
15	25.511916917479905	26.682634194664217	21.661998422719794	26.14345046513608
16	26.829281933089288	25.197930253237633	22.795052939856916	25.17773487381616
17	24.7073606653956	25.515490184868018	24.4161506586094	25.360998491126978
18	25.968270695753105	23.942004419137	25.45042637961331	24.639298505496583
19	26.10477652190663	24.424139238068015	24.708046205563804	24.763038034461545
20	26.166129083370254	23.767975279660337	24.161174327979452	25.904721308989952
21	27.522529324005323	23.648411436439748	23.744350969679008	25.084708269875915
22	26.408854815548494	23.58645381737471	24.47122700686431	25.53346436021248
23	24.60268439694277	23.39489218915056	25.2671347790965	26.735288634810168
24	25.25981402262451	24.504172186911983	24.55500401434725	25.681009776116248
25	25.345717195783475	23.979502743925362	24.663013899530885	26.01176616076028
26	25.051284298025	25.112496092698894	25.116473305753846	24.719746303522257
27	26.02744407692991	24.0639926825737	24.218593197453067	25.68997004304332
28	25.0297634581783	24.00817469845081	25.240593131662603	25.721468711708283
29	24.874664757743194	24.362138690249825	25.211149803266547	25.55204674874043
30	24.878246400206784	24.572728436347006	25.41801344353603	25.13101171991018
31	25.671574646193	24.480039171045238	23.91285436622939	25.93553181653237
32	25.406377769768973	24.182087066747858	23.83977282612748	26.571762337355686
33	24.726855157269167	23.740097470283867	24.94061123052039	26.59243614192658
34	25.87644593305598	24.078968218212776	24.826519103560344	25.2180667451709
35	26.520646489152266	23.772794161512916	25.023179449957244	24.683379899377574
36	24.81540769390426	24.77172198739515	24.391600413118887	26.0212699055817
37	26.362102659860376	24.35911377342252	24.208550949328746	25.07023261738836
38	25.019091594292593	23.900501259206376	24.966958648445612	26.113448498055426
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	68.0
2	96.0
3	96.0
4	267.0
5	438.0
6	438.0
7	549.0
8	660.0
9	628.5
10	597.0
11	597.0
12	938.0
13	1279.0
14	1830.5
15	2382.0
16	2382.0
17	3287.0
18	4192.0
19	4192.0
20	5555.5
21	6919.0
22	7855.0
23	8791.0
24	8791.0
25	10738.0
26	12685.0
27	12685.0
28	16352.0
29	20019.0
30	24734.5
31	29450.0
32	29450.0
33	39493.0
34	49536.0
35	49536.0
36	56030.5
37	62525.0
38	72067.0
39	81609.0
40	81609.0
41	87561.0
42	93513.0
43	107859.5
44	122206.0
45	122206.0
46	124838.0
47	127470.0
48	127470.0
49	138696.5
50	149923.0
51	154562.5
52	159202.0
53	159202.0
54	151251.0
55	143300.0
56	143300.0
57	140171.0
58	137042.0
59	123264.5
60	109487.0
61	109487.0
62	105048.5
63	100610.0
64	83848.5
65	67087.0
66	67087.0
67	56539.5
68	45992.0
69	45992.0
70	36988.0
71	27984.0
72	21770.5
73	15557.0
74	15557.0
75	11672.0
76	7787.0
77	7787.0
78	7713.0
79	7639.0
80	6078.0
81	4517.0
82	4517.0
83	4589.0
84	4661.0
85	4661.0
86	3072.5
87	1484.0
88	1284.0
89	1084.0
90	1084.0
91	759.5
92	435.0
93	362.0
94	289.0
95	289.0
96	258.0
97	227.0
98	227.0
99	481.0
100	735.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11854988881287944
2	0.05430429917319529
3	0.012923677606435494
4	0.00354158472868665
5	6.213306541555527E-5
6	3.727983924933316E-4
7	6.213306541555527E-5
8	1.2426613083111053E-4
9	1.2426613083111053E-4
10	5.591975887399974E-4
11	0.0016775927662199919
12	0.002609588747453321
13	0.013607141326006602
14	0.008822895289008847
15	0.02174657289544434
16	0.010562621120644395
17	0.018702052690082134
18	0.0052813105603221975
19	0.007393834784451076
20	0.005343443625737752
21	0.006337572672386637
22	0.006834637195711079
23	0.009257826746917733
24	0.013731407456837711
25	0.01603033087721326
26	0.017521524447186583
27	0.008698629158177738
28	0.005778375083646639
29	0.010251955793566619
30	0.0031687863361933187
31	0.00602690734530886
32	0.006772504130295523
33	0.008574363027346626
34	0.01205381469061772
35	0.016465262335122143
36	0.014104205849331045
37	0.00956849207399551
38	0.006337572672386637
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1609449.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.63225334363099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.46671882253777	41.24843546497085
2	10.819040245416813	10.955847732817677
3	3.1891016549444666	4.8441420879522346
4	1.3581497183001607	2.7506472246221927
5	0.7512398981625219	1.9018484422804178
6	0.47479677880466337	1.4424018474706588
7	0.3262507538112423	1.1563167574364985
8	0.24027137926910858	0.9732385077101724
9	0.177950616875121	0.810903664464889
>10	0.9866415511472774	9.06859760860158
>50	0.09333714269736318	3.2998891896677556
>100	0.09310277361143171	10.069721155144347
>500	0.016255914230124364	5.652333017596405
>1k	0.007142750192024342	5.825677299264355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2897	0.17999949050886357	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2803	0.1741589823598014	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2520	0.15657532484719927	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2486	0.15446280062307038	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2475	0.15377933690349926	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2443	0.15179107881020149	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2348	0.14588843759572376	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2347	0.1458263045303082	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2311	0.1435895141753482	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2096	0.13023090511100383	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2043	0.1269378526439794	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2023	0.12569519133566828	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2001	0.12432826389652607	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1951	0.12122161062574832	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1896	0.11780429202789278	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1855	0.115256836345855	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1849	0.11488403795336168	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1849	0.11488403795336168	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1837	0.11413844116837502	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1769	0.10991339272011726	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1639	0.10183609421609506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.213306541555527E-5
2	0.0	0.0	0.0	0.0	6.213306541555527E-5
3	0.0	0.0	0.0	6.213306541555527E-5	1.2426613083111053E-4
4	0.0	0.0	0.0	6.213306541555527E-5	1.2426613083111053E-4
5	0.0	6.213306541555527E-5	0.0	6.213306541555527E-5	1.2426613083111053E-4
6	0.0	6.213306541555527E-5	0.0	6.213306541555527E-5	2.4853226166222106E-4
7	0.0	6.213306541555527E-5	0.0	6.213306541555527E-5	2.4853226166222106E-4
8	0.0	6.213306541555527E-5	0.0	6.213306541555527E-5	2.4853226166222106E-4
9	0.0	6.213306541555527E-5	0.0	6.213306541555527E-5	2.4853226166222106E-4
10	0.0	6.213306541555527E-5	0.0	6.213306541555527E-5	2.4853226166222106E-4
11	0.0	6.213306541555527E-5	0.0	1.2426613083111053E-4	2.4853226166222106E-4
12	0.0	6.213306541555527E-5	0.0	1.2426613083111053E-4	3.1066532707777633E-4
13	0.0	6.213306541555527E-5	0.0	1.2426613083111053E-4	3.727983924933316E-4
14	0.0	6.213306541555527E-5	0.0	1.2426613083111053E-4	3.727983924933316E-4
15	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	4.970645233244421E-4
16	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	5.591975887399974E-4
17	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	5.591975887399974E-4
18	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	7.455967849866632E-4
19	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	7.455967849866632E-4
20	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	7.455967849866632E-4
21	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	7.455967849866632E-4
22	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	7.455967849866632E-4
23	0.0	6.213306541555527E-5	0.0	3.727983924933316E-4	7.455967849866632E-4
24	0.0	6.213306541555527E-5	0.0	5.591975887399974E-4	7.455967849866632E-4
25	0.0	6.213306541555527E-5	0.0	7.455967849866632E-4	7.455967849866632E-4
26	0.0	6.213306541555527E-5	0.0	8.077298504022183E-4	8.077298504022183E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	35	0.0020671545	22.856653	17
TCTAGCG	595	0.0	21.512146	28
CTAGCGG	630	0.0	20.317657	29
TAAGCTA	40	0.0044888337	19.996468	5
TAGCGGC	645	0.0	19.845154	30
TCCGTAC	50	7.204709E-4	19.197203	3
AGCGGCG	745	0.0	17.825676	31
CGCTTAC	45	0.008854441	17.776846	18
GTATCAA	2185	0.0	17.666983	1
CCTATTC	610	0.0	17.57121	3
CTATTCC	630	0.0	17.012865	4
GTTTTCG	635	0.0	16.881529	28
GTCCTAT	650	0.0	16.510424	1
TTTTCGG	645	0.0	16.124187	29
GATATAC	90	8.496061E-6	16.017576	1
ACGCGAA	60	0.002442317	16.000154	11
TTCGGAA	660	0.0	16.000154	31
AACCGCG	265	0.0	15.696803	7
GGTATCA	980	0.0	15.527243	1
CGTTATT	300	0.0	15.471624	2
>>END_MODULE