FastQCFastQC Report
Thu 2 Feb 2017
SRR4062989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609449
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC32710.20323725697428124No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT28700.1783218977426436No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25650.15937131279089925No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24410.15166681267937038No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24350.15129401428687705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24260.15073481669813707No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24030.1493057561935793No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22780.14153912301663488No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21930.1362578124563127No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21630.13439382049384604No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC20510.12743491716730385No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20080.12476319535443497No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA19950.12395546550403275No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG19590.12171867514907275No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC19480.12103521142950165No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18970.11786642509330834No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18910.11749362670081501No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG18890.1173693605699839No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18750.1164994976541661No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT18420.1144491064954528No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG17290.10742807010349505No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG17170.10668247331850839No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT16720.1038864853748084No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16290.10121476356193951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA3150.020.31698613
ATAACGA3450.018.55029112
ACGAACG3450.018.55029115
CTAGCGG6850.018.21855229
TCTAGCG6900.018.08653528
GATAACG3550.018.02886811
GGTATAT802.7957267E-618.0012591
TACCGTC4800.018.0012577
GTATCAA20400.017.8051661
ACCGTCG4700.017.7033658
CGATAAC3550.017.57814610
TAGCGGC7250.017.43407830
CGAACGA3700.017.29689416
CGGTCCA7800.017.23143810
CGTCGTA4850.017.15530410
ATACCGT4950.017.1325116
CGCCGGT7400.017.0822757
CAAGACG6150.016.9107024
CCGATAA3700.016.8660439
CCGTCGT4900.016.6542249
Produced by FastQC (version 0.11.5)