##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062989_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1609449
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.127136057122655	32.0	32.0	32.0	32.0	32.0
2	31.092135258712766	32.0	32.0	32.0	32.0	32.0
3	31.25276538740898	32.0	32.0	32.0	32.0	32.0
4	31.384639090769575	32.0	32.0	32.0	32.0	32.0
5	31.26294837549994	32.0	32.0	32.0	32.0	32.0
6	34.78527247523842	36.0	36.0	36.0	32.0	36.0
7	34.81782958018552	36.0	36.0	36.0	32.0	36.0
8	34.73631099836031	36.0	36.0	36.0	32.0	36.0
9	34.88866189608991	36.0	36.0	36.0	32.0	36.0
10	34.68468525563718	36.0	36.0	36.0	32.0	36.0
11	34.913374701528284	36.0	36.0	36.0	36.0	36.0
12	34.78389001453292	36.0	36.0	36.0	32.0	36.0
13	34.862203772844	36.0	36.0	36.0	32.0	36.0
14	34.797176549241385	36.0	36.0	36.0	32.0	36.0
15	34.75399655410019	36.0	36.0	36.0	32.0	36.0
16	34.771140309509654	36.0	36.0	36.0	32.0	36.0
17	34.722303720093024	36.0	36.0	36.0	32.0	36.0
18	34.73709014700062	36.0	36.0	36.0	32.0	36.0
19	34.72950867035861	36.0	36.0	36.0	32.0	36.0
20	34.7250512442457	36.0	36.0	36.0	32.0	36.0
21	34.714755795306345	36.0	36.0	36.0	32.0	36.0
22	34.69653713786519	36.0	36.0	36.0	32.0	36.0
23	34.62713761044929	36.0	36.0	36.0	32.0	36.0
24	34.602286869605685	36.0	36.0	36.0	32.0	36.0
25	34.575608795308206	36.0	36.0	36.0	32.0	36.0
26	34.50747616109613	36.0	36.0	36.0	32.0	36.0
27	34.496903598685016	36.0	36.0	36.0	32.0	36.0
28	34.45970950306596	36.0	36.0	36.0	32.0	36.0
29	34.424025240936494	36.0	36.0	36.0	32.0	36.0
30	34.40021212228533	36.0	36.0	36.0	32.0	36.0
31	34.41319047698933	36.0	36.0	36.0	32.0	36.0
32	34.384701845165644	36.0	36.0	36.0	32.0	36.0
33	34.34491369406548	36.0	36.0	36.0	32.0	36.0
34	34.34438183502553	36.0	36.0	36.0	32.0	36.0
35	34.3153190936774	36.0	36.0	36.0	32.0	36.0
36	34.278860032222205	36.0	36.0	36.0	32.0	36.0
37	34.281455330364615	36.0	36.0	36.0	32.0	36.0
38	33.79206858993357	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	9.0
21	36.0
22	133.0
23	384.0
24	1055.0
25	2719.0
26	5840.0
27	11420.0
28	19857.0
29	31934.0
30	49539.0
31	73036.0
32	106508.0
33	173170.0
34	400292.0
35	733516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18309201017571	18.317154677559866	11.915328318385008	26.58442499387941
2	16.302535836798803	20.096442944138023	35.93894556460006	27.66207565446311
3	18.33515693880328	23.753595174497608	28.1405623912283	29.77068549547081
4	12.244593633208964	15.607181357548718	35.5356038882969	36.61262112094542
5	14.816508517205898	36.33115204716152	32.61826415019311	16.23407528543948
6	35.54668156205206	35.29342687270845	15.769329465338094	13.390562099901397
7	31.321025992795036	29.798576653084353	20.075168722653633	18.805228631466978
8	28.816435431567395	32.54912852670031	18.550504193796325	20.083931847935972
9	27.301919677392295	13.987827648449564	18.045961656160582	40.664291017997556
10	16.13297072205995	25.897565421945686	30.28910109560051	27.68036276039385
11	38.80175695752321	20.811521008492125	21.23348658142022	19.153235452564445
12	25.30230899328092	23.34710204629419	27.402099852992894	23.948489107431996
13	29.35297571523103	18.549313583253856	25.297975389031624	26.7997353124835
14	23.82622872796839	19.452495854171207	23.919863257549633	32.80141216031077
15	25.517677167776053	26.89367603446894	21.345876756579425	26.242770041175582
16	26.864196211493763	25.325468934360686	22.530285247044482	25.280049607101073
17	24.825142020654273	25.720665892488668	24.055002674828465	25.39918941202859
18	26.07656409118897	24.022507081616133	25.253052442171203	24.647876385023697
19	26.13646036624957	24.571328448431732	24.504846068437086	24.787365116881617
20	26.252090000987916	23.709853496445056	24.005482621692266	26.03257388087476
21	27.584132935101312	23.83414924505125	23.50173693200211	25.079980887845327
22	26.384619829519295	23.64312258418875	24.38542631670839	25.586831269583566
23	24.562178538453605	23.33703647093472	25.19537778838184	26.905407202229835
24	25.352900278294	24.529077963949153	24.326772702956102	25.79124905480074
25	25.406584257306093	24.08383014020361	24.516712282805564	25.992873319684733
26	25.225216828865033	25.037202172917567	24.92654318341246	24.811037814804944
27	26.18382203090749	23.981638425099785	24.077571937707834	25.756967606284885
28	24.938037800514337	24.09992488112391	25.27672514009453	25.68531217826722
29	25.029062741348124	24.270728677951272	25.121578875751887	25.578629704948714
30	24.999425269144908	24.530009959930386	25.334881689323492	25.13568308160122
31	25.823620381882247	24.320186598022055	23.911848092111025	25.94434492798467
32	25.569371878201796	24.184177317827405	23.804109356680453	26.442341447290346
33	24.887523618331493	23.757261025357128	24.783761399087513	26.571453957223866
34	25.908556282305312	24.04015287219415	24.894047590200124	25.157243255300415
35	26.602831155258727	23.71848999253782	24.893612658742214	24.78506619346124
36	25.004209515181902	24.65353049397651	24.32149139239578	26.020768598445805
37	26.549910279797796	24.348285871926276	24.13473439340693	24.967069454868998
38	25.28039168781785	23.699349775854408	24.870001770796765	26.150256765530976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	40.0
2	58.0
3	58.0
4	183.5
5	309.0
6	309.0
7	420.0
8	531.0
9	527.0
10	523.0
11	523.0
12	881.5
13	1240.0
14	1805.5
15	2371.0
16	2371.0
17	3319.5
18	4268.0
19	4268.0
20	5467.0
21	6666.0
22	7681.0
23	8696.0
24	8696.0
25	10810.0
26	12924.0
27	12924.0
28	16719.0
29	20514.0
30	25173.5
31	29833.0
32	29833.0
33	40130.5
34	50428.0
35	50428.0
36	56262.5
37	62097.0
38	71836.5
39	81576.0
40	81576.0
41	87171.0
42	92766.0
43	107374.0
44	121982.0
45	121982.0
46	124333.5
47	126685.0
48	126685.0
49	138234.5
50	149784.0
51	154044.0
52	158304.0
53	158304.0
54	150580.5
55	142857.0
56	142857.0
57	140298.0
58	137739.0
59	123928.0
60	110117.0
61	110117.0
62	105575.5
63	101034.0
64	84544.0
65	68054.0
66	68054.0
67	57690.0
68	47326.0
69	47326.0
70	37880.5
71	28435.0
72	22168.5
73	15902.0
74	15902.0
75	11959.5
76	8017.0
77	8017.0
78	7850.0
79	7683.0
80	6111.0
81	4539.0
82	4539.0
83	4256.0
84	3973.0
85	3973.0
86	2570.0
87	1167.0
88	955.0
89	743.0
90	743.0
91	444.5
92	146.0
93	108.0
94	70.0
95	70.0
96	51.5
97	33.0
98	33.0
99	35.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007890899307775518
2	0.0
3	0.0
4	2.4853226166222106E-4
5	6.213306541555527E-5
6	1.2426613083111053E-4
7	1.863991962466658E-4
8	0.0029823871399466528
9	0.003976516186595537
10	7.455967849866632E-4
11	0.0047221129715822
12	1.863991962466658E-4
13	2.4853226166222106E-4
14	0.0
15	0.0
16	1.2426613083111053E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2426613083111053E-4
22	0.0
23	1.863991962466658E-4
24	0.0
25	2.4853226166222106E-4
26	0.0
27	6.213306541555527E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	6.213306541555527E-5
38	2.4853226166222106E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1609449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.75498964595837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98181300253165	38.99512464803409
2	11.719632898937258	11.427811612842381
3	3.388691061659625	4.956467928737001
4	1.4830565235305218	2.8922562179640643
5	0.8165669351796889	1.9905856234958843
6	0.5085143570956868	1.4875567329012833
7	0.34940588559075036	1.1924696233949765
8	0.2673039769419351	1.042592210250203
9	0.19870095422437084	0.8718896669266124
>10	1.0630400185331572	9.437528180708414
>50	0.09887383367576243	3.3506321360218925
>100	0.09954618232258335	10.406051504042669
>500	0.016526874748646888	5.5471165811689955
>1k	0.008327495028387966	6.40191733351169
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3271	0.20323725697428124	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2870	0.1783218977426436	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2565	0.15937131279089925	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2441	0.15166681267937038	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2435	0.15129401428687705	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2426	0.15073481669813707	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2403	0.1493057561935793	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2278	0.14153912301663488	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2193	0.1362578124563127	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2163	0.13439382049384604	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2051	0.12743491716730385	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2008	0.12476319535443497	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1995	0.12395546550403275	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1959	0.12171867514907275	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1948	0.12103521142950165	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1897	0.11786642509330834	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1891	0.11749362670081501	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1889	0.1173693605699839	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1875	0.1164994976541661	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1842	0.1144491064954528	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1729	0.10742807010349505	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1717	0.10668247331850839	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1672	0.1038864853748084	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1629	0.10121476356193951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2426613083111053E-4
2	0.0	0.0	0.0	0.0	1.2426613083111053E-4
3	0.0	0.0	0.0	0.0	1.2426613083111053E-4
4	0.0	0.0	0.0	6.213306541555527E-5	1.2426613083111053E-4
5	0.0	0.0	0.0	6.213306541555527E-5	1.2426613083111053E-4
6	0.0	0.0	0.0	6.213306541555527E-5	1.863991962466658E-4
7	0.0	0.0	0.0	6.213306541555527E-5	1.863991962466658E-4
8	0.0	0.0	0.0	6.213306541555527E-5	1.863991962466658E-4
9	0.0	0.0	0.0	6.213306541555527E-5	1.863991962466658E-4
10	0.0	0.0	0.0	6.213306541555527E-5	2.4853226166222106E-4
11	0.0	0.0	0.0	1.2426613083111053E-4	2.4853226166222106E-4
12	0.0	6.213306541555527E-5	0.0	1.2426613083111053E-4	3.1066532707777633E-4
13	0.0	6.213306541555527E-5	0.0	1.2426613083111053E-4	4.970645233244421E-4
14	0.0	6.213306541555527E-5	0.0	1.2426613083111053E-4	4.970645233244421E-4
15	0.0	6.213306541555527E-5	0.0	1.2426613083111053E-4	8.077298504022183E-4
16	0.0	6.213306541555527E-5	0.0	1.2426613083111053E-4	8.077298504022183E-4
17	0.0	6.213306541555527E-5	0.0	1.2426613083111053E-4	8.077298504022183E-4
18	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	8.698629158177736E-4
19	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	8.698629158177736E-4
20	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	9.319959812333289E-4
21	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	9.941290466488843E-4
22	0.0	6.213306541555527E-5	0.0	1.863991962466658E-4	9.941290466488843E-4
23	0.0	6.213306541555527E-5	0.0	4.970645233244421E-4	9.941290466488843E-4
24	0.0	6.213306541555527E-5	0.0	6.834637195711079E-4	9.941290466488843E-4
25	0.0	6.213306541555527E-5	0.0	8.698629158177736E-4	0.0010562621120644394
26	0.0	6.213306541555527E-5	0.0	9.319959812333289E-4	0.0010562621120644394
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGAA	315	0.0	20.316986	13
ATAACGA	345	0.0	18.550291	12
ACGAACG	345	0.0	18.550291	15
CTAGCGG	685	0.0	18.218552	29
TCTAGCG	690	0.0	18.086535	28
GATAACG	355	0.0	18.028868	11
GGTATAT	80	2.7957267E-6	18.001259	1
TACCGTC	480	0.0	18.001257	7
GTATCAA	2040	0.0	17.805166	1
ACCGTCG	470	0.0	17.703365	8
CGATAAC	355	0.0	17.578146	10
TAGCGGC	725	0.0	17.434078	30
CGAACGA	370	0.0	17.296894	16
CGGTCCA	780	0.0	17.231438	10
CGTCGTA	485	0.0	17.155304	10
ATACCGT	495	0.0	17.132511	6
CGCCGGT	740	0.0	17.082275	7
CAAGACG	615	0.0	16.910702	4
CCGATAA	370	0.0	16.866043	9
CCGTCGT	490	0.0	16.654224	9
>>END_MODULE