FastQCFastQC Report
Thu 2 Feb 2017
SRR4062988_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062988_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681124
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20480.30067946511942023No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA18430.27058215537846264No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14880.2184624238758288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT13340.19585273753384114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT8240.12097650354414174No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG7730.1134888801451718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA555.8283073E-520.3638699
AGGCCCG1400.019.42879310
CATCGTT751.5076184E-619.1988128
CGCGTAA601.13721726E-418.6668810
ATCGTTT701.786473E-518.2845829
TAATACC701.7876026E-518.2832374
ACGAACG450.00884234817.77928715
AATTTCG450.00884983217.77667818
TACCGTC1006.812479E-817.5976187
GCGTAAC652.0986363E-417.23096811
GTCGCGT652.1009927E-417.2284378
GGTCGCG652.1009927E-417.2284377
GTAGCCG1102.0153675E-716.00018312
GCCGAGA805.6184428E-515.99900821
ACTAGTA600.002442740815.9978358
ACCGTCG908.581543E-615.9978358
TAGAACT1407.421477E-1015.9978334
CTAGCGG1255.129732E-815.35904929
CTAATAC2000.015.1979443
TAATACT951.432522E-515.1558444
Produced by FastQC (version 0.11.5)