##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062988_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	681124
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22414274052889	32.0	32.0	32.0	32.0	32.0
2	30.878813549368395	32.0	32.0	32.0	32.0	32.0
3	30.940843370663785	32.0	32.0	32.0	32.0	32.0
4	30.993863085135747	32.0	32.0	32.0	32.0	32.0
5	30.914178622394747	32.0	32.0	32.0	32.0	32.0
6	34.54254438252066	36.0	36.0	36.0	32.0	36.0
7	34.48561495410527	36.0	36.0	36.0	32.0	36.0
8	34.456066736746905	36.0	36.0	36.0	32.0	36.0
9	34.604220376906405	36.0	36.0	36.0	32.0	36.0
10	34.3357215426266	36.0	36.0	36.0	32.0	36.0
11	34.592014082604635	36.0	36.0	36.0	32.0	36.0
12	34.431469159800564	36.0	36.0	36.0	32.0	36.0
13	34.50271756684539	36.0	36.0	36.0	32.0	36.0
14	34.4040952895508	36.0	36.0	36.0	32.0	36.0
15	34.361003282809	36.0	36.0	36.0	32.0	36.0
16	34.37596972063824	36.0	36.0	36.0	32.0	36.0
17	34.29202905785144	36.0	36.0	36.0	32.0	36.0
18	34.323158485092286	36.0	36.0	36.0	32.0	36.0
19	34.31024600513269	36.0	36.0	36.0	32.0	36.0
20	34.29338563903195	36.0	36.0	36.0	32.0	36.0
21	34.25296568613057	36.0	36.0	36.0	32.0	36.0
22	34.22958668318838	36.0	36.0	36.0	32.0	36.0
23	34.175382162425635	36.0	36.0	36.0	32.0	36.0
24	34.16588168967765	36.0	36.0	36.0	32.0	36.0
25	34.13496955033151	36.0	36.0	36.0	32.0	36.0
26	34.08483330494888	36.0	36.0	36.0	32.0	36.0
27	34.0787507120583	36.0	36.0	36.0	32.0	36.0
28	34.048522735948225	36.0	36.0	36.0	32.0	36.0
29	34.01124464855151	36.0	36.0	36.0	32.0	36.0
30	33.98576764289616	36.0	36.0	36.0	32.0	36.0
31	33.989141477910046	36.0	36.0	36.0	32.0	36.0
32	33.95232145688597	36.0	36.0	36.0	32.0	36.0
33	33.89351424997504	36.0	36.0	36.0	32.0	36.0
34	33.9020134366136	36.0	36.0	36.0	32.0	36.0
35	33.841997932828676	36.0	36.0	36.0	32.0	36.0
36	33.799406569141595	36.0	36.0	36.0	32.0	36.0
37	33.779996006600854	36.0	36.0	36.0	32.0	36.0
38	33.14661941144344	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	5.0
12	4.0
13	2.0
14	83.0
15	173.0
16	241.0
17	241.0
18	356.0
19	471.0
20	644.0
21	890.0
22	1254.0
23	1811.0
24	2631.0
25	4154.0
26	6053.0
27	8883.0
28	12757.0
29	17627.0
30	24518.0
31	33579.0
32	45892.0
33	69635.0
34	151377.0
35	297833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.40978753303719	16.69337080732742	11.1644377283939	25.732403931241493
2	16.386562645517724	19.32989501424146	37.977610284990725	26.305932055250096
3	19.75570219101569	23.744079030443988	27.848599376848238	28.651619401692084
4	12.496347835789887	15.010798660408192	36.06326796387624	36.42958553992568
5	14.229777088065115	36.897849280817944	33.924809248283346	14.947564382833594
6	34.52166495625607	35.17441049560799	16.88593690126453	13.417987646871415
7	30.360257456792006	30.458477457849085	20.616363540265795	18.56490154509311
8	28.547350617584833	32.99604622379871	19.21611578559463	19.24048737302183
9	27.331146751545976	14.31031647688233	18.308120107351968	40.05041666421973
10	15.360390649545897	27.22992356729044	32.31183253514055	25.097853248023117
11	37.675082365792214	21.343017553750656	21.416574170322473	19.56532591013466
12	24.3570374611844	23.897930568708205	29.11416007810837	22.63087189199903
13	30.047986561413403	19.6298515177145	24.873132224415	25.4490296964571
14	23.39020629909698	19.45818956023787	25.687834960722416	31.463769179942737
15	25.25442097750522	26.991238813553846	23.11199809679892	24.642342112142014
16	25.671623820179846	25.990843362171752	24.057024885615444	24.28050793203296
17	23.838991102541772	25.951972228300058	25.679424242379746	24.529612426778428
18	24.834382634843074	25.0817809152415	26.576888742717536	23.506947707197895
19	24.860366365766705	25.871865951711136	25.706390551310587	23.561377131211568
20	25.159377817191952	24.326883829648533	26.30446081698934	24.209277536170177
21	26.219425353006038	24.712698526896293	24.766583612180085	24.301292507917584
22	24.94622456036284	24.893954098899822	25.488310357333212	24.67151098340413
23	23.921045792324534	24.974708282615303	25.574808421664684	25.529437503395474
24	23.978361678320855	25.67876725001248	25.60475996088186	24.7381111107848
25	24.6799950073788	24.95708548395386	25.41611295071255	24.946806557954787
26	24.050883844687682	25.579366874106096	25.955136695633783	24.41461258557244
27	24.973570610518898	25.443573253457846	25.294394032831175	24.28846210319208
28	24.272109802401488	25.119883478837963	25.77399358076461	24.834013137995942
29	23.863571291180353	25.792332235533195	26.173796983814913	24.170299489471546
30	24.391468594508645	25.425080053501137	26.146993056891212	24.03645829509901
31	24.164754282492545	25.703764717282812	25.343017852344857	24.788463147879785
32	23.99577135977212	25.745664909627497	25.318689708840502	24.939874021759877
33	23.83908565790285	25.31531822546685	25.795600374711114	25.04999574191919
34	24.33763068248561	25.645777046869494	25.862504405027604	24.154087865617292
35	24.885572855252782	25.363252036340622	25.864866571414723	23.88630853699187
36	24.033179593580815	26.024454458819918	25.57630381441982	24.366062133179447
37	24.973203456974296	25.804703857821217	25.262899782397387	23.959192902807104
38	23.913825480888526	25.901688927781198	25.49028532498976	24.69420026634052
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	68.5
2	93.0
3	93.0
4	268.5
5	444.0
6	444.0
7	575.0
8	706.0
9	720.5
10	735.0
11	735.0
12	946.0
13	1157.0
14	1468.0
15	1779.0
16	1779.0
17	2478.5
18	3178.0
19	3178.0
20	3904.5
21	4631.0
22	4928.5
23	5226.0
24	5226.0
25	6213.5
26	7201.0
27	7201.0
28	9247.0
29	11293.0
30	13843.5
31	16394.0
32	16394.0
33	19647.0
34	22900.0
35	22900.0
36	26018.5
37	29137.0
38	33462.5
39	37788.0
40	37788.0
41	41304.0
42	44820.0
43	50299.0
44	55778.0
45	55778.0
46	60416.5
47	65055.0
48	65055.0
49	65707.5
50	66360.0
51	64749.0
52	63138.0
53	63138.0
54	60338.0
55	57538.0
56	57538.0
57	54625.0
58	51712.0
59	46490.5
60	41269.0
61	41269.0
62	37581.5
63	33894.0
64	28170.5
65	22447.0
66	22447.0
67	18437.5
68	14428.0
69	14428.0
70	11580.5
71	8733.0
72	6801.0
73	4869.0
74	4869.0
75	3694.5
76	2520.0
77	2520.0
78	2361.5
79	2203.0
80	1700.5
81	1198.0
82	1198.0
83	1147.0
84	1096.0
85	1096.0
86	742.0
87	388.0
88	341.5
89	295.0
90	295.0
91	218.0
92	141.0
93	126.5
94	112.0
95	112.0
96	97.0
97	82.0
98	82.0
99	212.0
100	342.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1218574004146088
2	0.0535878929534123
3	0.010277130155448934
4	0.00308313904663468
5	4.4044843523352573E-4
6	1.468161450778419E-4
7	0.0
8	4.4044843523352573E-4
9	0.0
10	2.936322901556838E-4
11	0.0023490583212454706
12	0.0027895067564789964
13	0.01468161450778419
14	0.010864394735760303
15	0.024371480082921757
16	0.012919820766850087
17	0.020994708746131394
18	0.005285381222802309
19	0.008221704124359148
20	0.005579013512957993
21	0.006606726528502887
22	0.007487623398969938
23	0.010423946300526777
24	0.016149775958562613
25	0.017471121264263187
26	0.017911569699496716
27	0.009396233284981882
28	0.006753542673580728
29	0.009543049430059725
30	0.002495874466323313
31	0.005432197367880151
32	0.007928071834203463
33	0.009689865575137567
34	0.01233255618653872
35	0.018058385844574556
36	0.015122062943017718
37	0.009689865575137567
38	0.006019461948191519
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	681124.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.22386023828187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.54817217954381	53.188865849259116
2	12.20917695921487	15.92659303224564
3	3.250168554957871	6.359656187382921
4	1.183756007900969	3.0883654566223715
5	0.5503659622397195	1.7948496300515493
6	0.29507191133439975	1.1547437463070553
7	0.17526333832599036	0.8001946038708374
8	0.1323478979863043	0.6905792640871279
9	0.08460213322596347	0.496626994205167
>10	0.45330143857293426	5.6585407883954355
>50	0.06578158501974535	3.060425896078304
>100	0.04927940393802341	6.03604795330237
>500	0.0018084184929524235	0.7547455841907845
>1k	9.042092464762117E-4	0.989765014001316
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2048	0.30067946511942023	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1843	0.27058215537846264	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1488	0.2184624238758288	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1334	0.19585273753384114	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	824	0.12097650354414174	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	773	0.1134888801451718	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.468161450778419E-4	0.0
10	0.0	0.0	0.0	1.468161450778419E-4	0.0
11	0.0	0.0	0.0	1.468161450778419E-4	0.0
12	0.0	0.0	0.0	1.468161450778419E-4	0.0
13	0.0	0.0	0.0	1.468161450778419E-4	0.0
14	0.0	0.0	0.0	1.468161450778419E-4	0.0
15	0.0	0.0	0.0	1.468161450778419E-4	0.0
16	0.0	0.0	0.0	1.468161450778419E-4	0.0
17	0.0	0.0	0.0	1.468161450778419E-4	0.0
18	0.0	0.0	0.0	1.468161450778419E-4	0.0
19	0.0	0.0	0.0	1.468161450778419E-4	0.0
20	0.0	0.0	0.0	2.936322901556838E-4	1.468161450778419E-4
21	0.0	0.0	0.0	5.872645803113676E-4	1.468161450778419E-4
22	0.0	0.0	0.0	7.340807253892095E-4	1.468161450778419E-4
23	0.0	0.0	0.0	0.001468161450778419	1.468161450778419E-4
24	0.0	0.0	0.0	0.0026426906114011546	1.468161450778419E-4
25	0.0	0.0	0.0	0.002936322901556838	1.468161450778419E-4
26	0.0	0.0	0.0	0.003670403626946048	1.468161450778419E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	55	5.8283073E-5	20.363869	9
AGGCCCG	140	0.0	19.428793	10
CATCGTT	75	1.5076184E-6	19.19881	28
CGCGTAA	60	1.13721726E-4	18.66688	10
ATCGTTT	70	1.786473E-5	18.28458	29
TAATACC	70	1.7876026E-5	18.283237	4
ACGAACG	45	0.008842348	17.779287	15
AATTTCG	45	0.008849832	17.776678	18
TACCGTC	100	6.812479E-8	17.597618	7
GCGTAAC	65	2.0986363E-4	17.230968	11
GTCGCGT	65	2.1009927E-4	17.228437	8
GGTCGCG	65	2.1009927E-4	17.228437	7
GTAGCCG	110	2.0153675E-7	16.000183	12
GCCGAGA	80	5.6184428E-5	15.999008	21
ACTAGTA	60	0.0024427408	15.997835	8
ACCGTCG	90	8.581543E-6	15.997835	8
TAGAACT	140	7.421477E-10	15.997833	4
CTAGCGG	125	5.129732E-8	15.359049	29
CTAATAC	200	0.0	15.197944	3
TAATACT	95	1.432522E-5	15.155844	4
>>END_MODULE