FastQCFastQC Report
Thu 2 Feb 2017
SRR4062988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681124
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23330.3425220664666052No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19330.2837956084354684No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17360.2548728278551336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16740.2457702268603074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10440.15327605546126696No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG8670.12728959778248894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.11025892495345928No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC6850.10056905937832171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCCG308.41062E-426.6681355
AAGACGG1700.022.5894785
CGCAAGA1700.021.6482492
GCGCAAG1850.019.8929861
CAAGACG1900.019.3694884
GACGGAC2000.019.2010577
GTCCCGG701.7846745E-518.286721
AGACGGA2200.018.182826
ATAGACG550.001363368817.4555073
CAACGGA652.0991158E-417.23045314
AAACGCT652.0991158E-417.23045329
AACGCTT652.0991158E-417.23045330
CGCGTAA652.0991158E-417.23045310
ACTGTTC753.229041E-517.0676068
ATCATAG753.229041E-517.0676061
ATCGAAC753.229041E-517.0676068
TAGCGGC1800.016.88857830
CGTTTAT1252.8467184E-916.63969631
AATAACG1354.4201443E-1016.5935062
TTAGGAC703.6920886E-416.0008813
Produced by FastQC (version 0.11.5)